Abstract: Provided are 3D cell culture scaffolds, 3D nanofiber scaffolds, and edible 3D nanofiber scaffolds for cultured meat. Described is a 3D cell culture scaffold including a plurality of laminated nanofiber layers. Each layer is formed by an array of nanofibers. The diameter of each of the nanofibers in the arrays can have a tunable, predetermined diameter and can be formed from materials including a natural polymer, a synthetic polymer, a biocompatible material, or a combination thereof. Each of the nanofibers in the arrays can have controlled alignment, angle, and spacing from one another. The layers can be spaced by spacer fibers or spacer sheets. The scaffold can have a porosity of about 50% to 99%. Edible 3D scaffolds for cultured meat are also provided where the nanofibers and spacers are edible.

Abstract: Nanofiber spinning apparatuses and methods for making core-sheath materials using touch spinning are provided. The apparatus includes at least one rotating plate with an aperture through which a core yarn passes and at least one post contacting the rotating plate. A speed control device can be configured to control rotation of the rotating plate, and a dispensing device can be configured to dispense a nanofiber-forming material onto the post. To make a core-sheath yarn a core yarn is passed through an aperture in a rotating plate having at least one post. The post is contacted with a nanofiber-forming material the rotating plate is rotated to draw a fiber of nanofiber-forming material from the post to wrap the fiber around the core yarn.

Abstract: Provided are methods for forming single filament nanofibers, methods for forming 3D nanofiber scaffolds, apparatus for forming nanofibers and nanofiber scaffolds, and nanofiber cell culture scaffolds formed using the methods and devices. Single filament nanofibers (having a diameter of about 50 nm-100 ?m) can be formed by gravitational drawing by dispensing a droplet of a polymer solution from a nozzle such that the droplet free falls from the nozzle onto a base, causing the polymer solution to be drawn into a fluid tail. Nanofiber scaffolds can be built by forming and collecting single filament nanofibers in an ordered manner on a collection frame to form 2D arrays that can then be stacked. The spacing and alignment of individual fibers is precisely controlled. Device for forming the 3D nanofiber scaffolds are provided. The 3D nanofiber scaffolds can be cell culture scaffolds having a porosity of 50% or greater.

Abstract: A simple controllable set-up for drawing single filament nanofibers from polymer solutions or melts using a rotating rod or a set of rods (round brush) is described. The set-up can be assembled in a few minutes and applied to fabricate customized nanofiber scaffolds and meshes for various applications. The resulting fiber diameter is controlled precisely in the range 40 nm to 5 ?m by adjusting the rotational speed and polymer concentration. Owing to the simple design and capability to manipulate single nanofibers, the spinning set-up can be used to wind a single filament into unidirectional, orthogonal or randomly oriented 2D and 3D meshes with controlled density, thickness and combinations of different fibers and materials in the scaffolds. The method is scalable and can be implemented easily for laboratory and industrial manufacturing.

Abstract: Nanofiber spinning apparatuses and methods for making core-sheath materials using touch spinning are provided. The apparatus includes at least one rotating plate with an aperture through which a core yarn passes and at least one post contacting the rotating plate. A speed control device can be configured to control rotation of the rotating plate, and a dispensing device can be configured to dispense a nanofiber-forming material onto the post. To make a core-sheath yarn a core yarn is passed through an aperture in a rotating plate having at least one post. The post is contacted with a nanofiber-forming material the rotating plate is rotated to draw a fiber of nanofiber-forming material from the post to wrap the fiber around the core yarn.

Abstract: Briefly described, embodiments of the present disclosure relate to structures including single-walled carbon nanotube/quantum dot networks, devices including the structures, and methods of making devices including the single-walled carbon nanotube/quantum dot networks.

Abstract: Briefly described, embodiments of the present disclosure relate to structures including single-walled carbon nanotube/quantum dot networks, devices including the structures, and methods of making devices including the single-walled carbon nanotube/quantum dot networks.

Abstract: Briefly described, embodiments of the present disclosure relate to structures including single-walled carbon nanotube/quantum dot networks, devices including the structures, and methods of making devices including the single-walled carbon nanotube/quantum dot networks.