Abstract: A method of preventing or treating or controlling a respiratory disease in a bovine by delivering to a lung or trachea of the bovine a composition including a polyplex having one or more mRNA encoded with a molecule selected from lycosylphosphatidylinositol (GPI) complex, antibody immunoglobulin G (IgG), antibody immunoglobulin A (IgA), antibody immunoglobulin M (IgM), antimicrobial peptide Bactenecin 5 (Bac5), antimicrobial peptide Bactenecin 7 (Bac7), bovine myeloid antimicrobial peptide (BMAP-28), lipoxin inducing enzymes, resolvin inducing enzymes, cytokines, CRISPR associated protein 13 (Cas13) enzymes, nanoluciferase (NLuc) proteins, and gene activator, catalytically dead CRISPR-associated protein 9 fused to an activator selected from the group consisting of VP64-p65-Rta transactivation domain (dCas9-VPR), VP64, and VP64-p65-HSF1 on the N terminus and SS18 on the C-terminus, and a polymer prepared by polymerizing a diacrylate monomer; one or more linker monomers; and one or more branching monomers; and

Abstract: The present disclosure relates to anti-SARS-COV-1 and anti-SARS-COV-2 activatable RNase guide sequences and methods of use for screening, treating, and/or preventing SARS infections and/or COVID-related diseases.

Abstract: A synthetic mRNA construct encoding for an antibody against a cell surface antigen of Tritichomonas foetus (Tf), said synthetic mRNA construct comprising a peptide sequence selected from the group consisting of SEQ ID NO: 7, SEQ ID NO: 13, SEQ ID NO: 17, SEQ ID NO: 23, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO: 31, SEQ ID NO: 35, SEQ ID NO: 37, SEQ ID NO: 39, SEQ ID NO: 41, and SEQ ID NO: 44 for administration to a mammal to induce expression of the antibody. Also disclosed is a method for administering the synthetic mRNA construct to the preputial tissue of a male bovine to treat Tritichomonas foetus.

Abstract: Synthetic H2 RLN mRNA constructs that induce expression of relaxin, methods of preparing such synthetic H2 RLN mRNA constructs, and methods of administering such constructs to mammals to facilitate delivery of offspring or methods of administering such constructs to non-pregnant mammals to facilitate procedures involving the reproductive tract.

Abstract: The present disclosure relates to nanoparticle compositions for modifying antigen-specific T cells and uses thereof.

Abstract: Provided herein are branched BAE/BTE-containing polymers useful as vehicles for the delivery of therapeutic agents, such as nucleic acids. The polymers form stable compositions and are suitable for the delivery of therapeutic agents via nebulization. Compositions of the disclosed polymers are capable of delivering therapeutic agents such as mRNA to lung epithelial cells.

Abstract: Prophylactic compositions for inhibiting or reducing HIV infection and methods of their use are provided. One embodiment provides an antibody or an antigen binding fragment thereof that specifically binds to an HIV protein, for example gp120, and Cinhibits or reduces the ability of the HIV vims to infect human cells. One embodiment provides a PGT121 antibody or antigen binding fragment thereof modified to contain a GPI membrane anchor, wherein the antibody specifically binds to an HIV protein. In some embodiments the GPI membrane anchor is in the heavy chain of the antibody or antigen fragment thereof. In some aspects the antibody or antigen binding fragment specifically to gp120 or gp41.

Abstract: Non-hormonal contraception compositions and methods for contraception are provided. One embodiment provides an antibody or an antigen binding fragment thereof that specifically binds to one or more sperm antigens and inhibits the ability of anti-body-bound sperm to fertilize an egg. Typically, the antibody is a monoclonal antibody, for example a human or humanized monoclonal antibody. In one embodiment, the antibody or antigen binding fragment thereof specifically binds to CD52g expressed on vertebrate, for example human, sperm cells and inhibits, blocks, or reduces the ability of the antibody-bound sperm to fertilize an egg. In one embodiment the antibody contains a membrane anchor. The membrane anchor can contain transmembrane domains, glycosylphosphatidylinositol anchors, or myristoylation motifs.

Abstract: Synthetic oligonucleotides are used to express opsins in cells, such as neurons and cardiomyocytes, for rapid induction of light-responsive electrophysiological behavior. Such induction can change the electrical properties of the cell. MEA analysis and use of voltage indicator proteins are described. Methods of testing drugs for their effect on electrical properties of the cell are described, along with methods for screening drugs that have an effect on a cell's electrical properties.