Abstract: A mobile transport system for transporting objects in a technical system, includes a vehicle frame, a first wheel pair, a second wheel pair, and a third wheel pair, each having two wheels rotatable relative to the vehicle frame, and a swing frame pivotable about a swing axis extending in a transverse direction relative to the vehicle frame. The first wheels of the first wheel pair are attached to the vehicle frame, and the second wheels of the second wheel pair are attached to the swing frame. The third wheels of the third wheel pair are attached to a tilt frame pivotable relative to the swing frame about a tilt axis extending approximately in the longitudinal direction.

Abstract: Provided herein are E. coli host strains with improved capacity for producing recombinant proteins.

Abstract: Provided herein are methods for the improved production of periplasmic-targeted recombinant proteins in E. coli host strains. Also provided are E. coli host strains with improved capacity for producing recombinant proteins.

Abstract: A mobile transport system for transporting objects in a technical system, includes a vehicle frame, a first wheel pair, a second wheel pair, and a third wheel pair, each having two wheels rotatable relative to the vehicle frame, and a swing frame pivotable about a swing axis extending in a transverse direction relative to the vehicle frame. The first wheels of the first wheel pair are attached to the vehicle frame, and the second wheels of the second wheel pair are attached to the swing frame. The third wheels of the third wheel pair are attached to a tilt frame pivotable relative to the swing frame about a tilt axis extending approximately in the longitudinal direction.

Abstract: The present invention relates to the use of redox agents for purification of the CRM 197 variant of diphtheria toxin. The invention further relates to multi-step purification of CRM 197 from bacterial fermentates.

Abstract: Provided herein are methods for the improved production of periplasmic-targeted recombinant proteins in E. coli host strains. Also provided are E. coli host strains with improved capacity for producing recombinant proteins.

Abstract: The present invention relates to the use of redox agents for purification of the CRM 197 variant of diphtheria toxin. The invention further relates to multi-step purification of CRM 197 from bacterial fermentates.

Abstract: Rediced genome or native K12 strain E. coli bacteria comprising expression vectors encoding a recombinant CRM197 protein and their use in the production of CRM 197 is provided. The CRM 197 protein may be fused to a signal sequence that directs the expressed CRM197 protein to the periplasm of the E. coli host.

Abstract: E. coli bacteria comprising genetic modifications to enhance fermentability and production of protein and nucleic acids are provided.

Abstract: E. coli bacteria comprising genetic modifications to enhance fermentability and production of protein and nucleic acids are provided.

Abstract: Rediced genome or native K12 strain E. coli bacteria comprising expression vectors encoding a recombinant CRM197 protein and their use in the production of CRM 197 is provided. The CRM 197 protein may be fused to a signal sequence that directs the expressed CRM197 protein to the periplasm of the E. coli host.

Abstract: E. coli bacteria comprising genetic modifications to enhance fermentability and production of protein and nucleic acids are provided.

Abstract: The present invention provides a bacterium having a genome that is genetically engineered to be smaller than the genome of its native parent strain. A bacterium with a smaller genome can produce a commercial product more efficiently. The present invention also provides methods for deleting genes and other DNA sequences from a bacterial genome. The methods provide precise deletions and seldom introduces mutations to the genomic DNA sequences around the deletion sites. Thus, the methods can be used to generate a series of deletions in a bacterium without increasing the possibility of undesired homologous recombination within the genome. In addition, some of the methods provided by the present invention can also be used for replacing a region of a bacterial genome with a desired DNA sequence.

Abstract: A makeup mirror having a light source configured to have output that not only enhances the visibility of objects as they are reflected by the mirror, but also optimizes visual acuity of the reflected image as captured by the human eye. In some cases, the makeup mirror allows for separate adjustment of the mirror and the light source, which, in combination with the light source, enables the reflected objects to be lit in uniform manner and for optimized human visual acuity while allowing for areas of the objects to be examined in detail as desired.

Abstract: The present invention provides a bacterium having a genome that is genetically engineered to be smaller than the genome of its native parent strain. A bacterium with a smaller genome can produce a commercial product more efficiently. The present invention also provides methods for deleting genes and other DNA sequences from a bacterial genome. The methods provide precise deletions and seldom introduces mutations to the genomic DNA sequences around the deletion sites. Thus, the methods can be used to generate a series of deletions in a bacterium without increasing the possibility of undesired homologous recombination within the genome. In addition, some of the methods provided by the present invention can also be used for replacing a region of a bacterial genome with a desired DNA sequence.

Abstract: A bacteria lacking genomic and non-genomic IS elements is provided. The bacteria may be more stable and useful for the production of amino acids, polypeptides, nucleic acids and other products.

Abstract: Provided herein is a nucleic acid comprising a mutant lac operator operably linked to a gene of interest, a host cell comprising the nucleic acid, and a method of using the host cell to express the gene of interest. Also provided is a recA-mediated cloning method.

Abstract: The present invention relates to cloning target nucleic acids using phage packaging mechanisms. Packaging initiation sites may be introduced into the target DNA. Components of a phage packaging system may be combined with the target DNA to package the DNA into phage capsids. The packaged DNA may be used to create a library of target nucleic acids, or it may be sequenced.

Abstract: The present invention relates generally to the field of molecular biology and genomics. More specifically, the present invention concerns the cloning of nucleic acid molecules and the production of nucleic acid libraries, as well as the expression of recombinant proteins and bactofection.