Patents by Inventor David A. Tirrell

David A. Tirrell has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20210223252
    Abstract: The invention provides methods, reagents and systems for incorporating non-natural amino acids into proteins, preferably in vivo, using the endogenous protein synthesis machinery of an organism. The incorporated non-natural amino acids contain reactive groups for further chemical reagents, which may serve as a “handle” to enrich the proteins or fragments thereof in a number of uses, such as proteomic analysis, imaging of diseased tissues/cells, etc.
    Type: Application
    Filed: October 9, 2020
    Publication date: July 22, 2021
    Applicant: California Institute of Technology
    Inventors: Daniela C. Dieterich, David Tirrell, Erin Schuman, Aaron J. Link
  • Patent number: 10640546
    Abstract: In some aspects, the invention relates to an insulin derivative, comprising a cyclic amino acid at position B28 or B29, wherein the cyclic amino acid is not L-proline. In some aspects, the invention relates to a method of making said insulin derivative. In some aspects, the invention relates to a pharmaceutical composition comprising said insulin derivative. In some aspects the invention relates to a method of treating a disease or condition in a subject comprising administering to the subject a composition comprising said insulin derivative.
    Type: Grant
    Filed: June 10, 2015
    Date of Patent: May 5, 2020
    Assignee: California Institute of Technology
    Inventors: David A. Tirrell, Seth Lieblich, Katharine Y. Fang, Howard C. Zisser
  • Patent number: 10407482
    Abstract: The invention provides compositions and methods of identifying, modifying and producing modified target molecules, including therapeutic molecules by modification with non-natural amino acids. Certain aspects of the invention include methods of adding a chemical moiety to a target molecule, and the compositions resulting therefrom. Certain aspects of the invention also relate to kits for identifying, modifying and producing modified target molecules described herein.
    Type: Grant
    Filed: October 16, 2013
    Date of Patent: September 10, 2019
    Assignee: ALLOZYNE, INC.
    Inventors: Kenneth H. Grabstein, David A. Tirrell
  • Publication number: 20170292958
    Abstract: The invention provides methods, reagents and systems for incorporating non-natural amino acids into proteins, preferably in vivo, using the endogenous protein synthesis machinery of an organism. The incorporated non-natural amino acids contain reactive groups for further chemical reagents, which may serve as a “handle” to enrich the proteins or fragments thereof in a number of uses, such as proteomic analysis, imaging of diseased tissues/cells, etc.
    Type: Application
    Filed: April 24, 2017
    Publication date: October 12, 2017
    Applicant: California Institute of Technology
    Inventors: Daniela C. Dieterich, David Tirrell, Erin Schuman, Aaron J. Link
  • Patent number: 9783784
    Abstract: In some embodiments, methods for improving the survival of pancreatic ?-cell progenitors in culture are provided. Such methods may include contacting a population of pancreatic progenitor cells with an amino acid (aa) sequence comprising IKVAV (SEQ ID NO:1). In other embodiments, methods for (i) verifying the establishment of a population of pancreatic progenitor or stem cells and (ii) methods for generating or establishing a population of pancreatic endocrine progenitor cells in vitro are provided.
    Type: Grant
    Filed: March 14, 2013
    Date of Patent: October 10, 2017
    Assignees: CITY OF HOPE, CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Hsun Ku, David Tirrell
  • Publication number: 20150353619
    Abstract: In some aspects, the invention relates to an insulin derivative, comprising a cyclic amino acid at position B28 or B29, wherein the cyclic amino acid is not L-proline. In some aspects, the invention relates to a method of making said insulin derivative. In some aspects, the invention relates to a pharmaceutical composition comprising said insulin derivative. In some aspects the invention relates to a method of treating a disease or condition in a subject comprising administering to the subject a composition comprising said insulin derivative.
    Type: Application
    Filed: June 10, 2015
    Publication date: December 10, 2015
    Inventors: David A. Tirrell, Seth Lieblich, Katherine Y. Fang, Howard C. Zisser
  • Patent number: 9133457
    Abstract: The invention provides a method of incorporating nonstandard amino acids into a protein by utilizing a modified aminoacyl-tRNA synthetase to charge the nonstandard amino acid to a modified tRNA, which forms strict Watson-Crick base-pairing with a codon that normally forms wobble base-pairing with natural tRNAs.
    Type: Grant
    Filed: August 8, 2014
    Date of Patent: September 15, 2015
    Assignee: California Institute of Technology
    Inventors: Inchan Kwon, David Tirrell
  • Patent number: 8980581
    Abstract: The invention provides certain embodiments relating to methods and compositions for incorporating non-natural amino acids into a polypeptide or protein by utilizing a mutant or modified aminoacyl-tRNA synthetase to charge the non-natural amino acid to a the corresponding tRNA. In certain embodiments, the tRNA is also modified such that the complex forms strict Watson-Crick base-pairing with a codon that normally forms wobble base-pairing with unmodified tRNA/aminoacyl-tRNA synthetase pairs.
    Type: Grant
    Filed: August 16, 2013
    Date of Patent: March 17, 2015
    Assignee: California Institute of Technology
    Inventors: Pin Wang, Inchan Kwon, Soojin Son, Yi Tang, David Tirrell
  • Publication number: 20140342451
    Abstract: The invention provides a method of incorporating nonstandard amino acids into a protein by utilizing a modified aminoacyl-tRNA synthetase to charge the nonstandard amino acid to a modified tRNA, which forms strict Watson-Crick base-pairing with a codon that normally forms wobble base-pairing with natural tRNAs.
    Type: Application
    Filed: August 8, 2014
    Publication date: November 20, 2014
    Inventors: Inchan Kwon, David Tirrell
  • Patent number: 8846106
    Abstract: Compositions and methods for transport or release of therapeutic and diagnostic agents or metabolites or other analytes from cells, compartments within cells, or through cell layers or barriers are described. The compositions include a membrane barrier transport enhancing agent and are usually administered in combination with an enhancer and/or exposure to stimuli to effect disruption or altered permeability, transport or release. In a preferred embodiment, the compositions include compounds which disrupt endosomal membranes in response to the low pH in the endosomes but which are relatively inactive toward cell membranes, coupled directly or indirectly to a therapeutic or diagnostic agent. Other disruptive agents can also be used, responsive to stimuli and/or enhancers other than pH, such as light, electrical stimuli, electromagnetic stimuli, ultrasound, temperature, or combinations thereof.
    Type: Grant
    Filed: July 14, 2011
    Date of Patent: September 30, 2014
    Assignees: University of Washington, University of Massachusetts
    Inventors: Allan S. Hoffman, Patrick Stayton, Oliver W. Press, Niren Murthy, Chantal Lackey Reed, Lawrence A. Crum, Pierre D. Mourad, Tyrone M. Porter, David Tirrell
  • Patent number: 8835162
    Abstract: The invention provides a method of incorporating nonstandard amino acids into a protein by utilizing a modified aminoacyl-tRNA synthetase to charge the nonstandard amino acid to a modified tRNA, which forms strict Watson-Crick base-pairing with a codon that normally forms wobble base-pairing with natural tRNAs.
    Type: Grant
    Filed: December 28, 2012
    Date of Patent: September 16, 2014
    Assignee: California Institute of Technology
    Inventors: Inchan Kwon, David Tirrell
  • Publication number: 20140255345
    Abstract: The invention provides compositions and methods of identifying, modifying and producing modified target molecules, including therapeutic molecules by modification with non-natural amino acids. Certain aspects of the invention include methods of adding a chemical moiety to a target molecule, and the compositions resulting therefrom. Certain aspects of the invention also relate to kits for identifying, modifying and producing modified target molecules described herein.
    Type: Application
    Filed: October 16, 2013
    Publication date: September 11, 2014
    Applicant: Allozyne, Inc.
    Inventors: Kenneth H. Grabstein, David A. Tirrell
  • Publication number: 20140255991
    Abstract: Methods for producing modified polypeptides containing amino acid analogues are disclosed. The invention further provides purified dihydrofolate reductase polypeptides, produced by the methods of the invention, in which the methionine residues have been replaced with homoallylglycine, homoproparglycine, norvaline, norleucine, cis-crotylglycine, trans-crotylglycine, 2-aminoheptanoic acid, 2-butynylglycine and allylglycine.
    Type: Application
    Filed: January 9, 2014
    Publication date: September 11, 2014
    Applicant: California Institute of Technology
    Inventors: David A. Tirrell, Kristi Lynn Kiick
  • Publication number: 20140219950
    Abstract: Compositions and methods for transport or release of therapeutic and diagnostic agents or metabolites or other analytes from cells, compartments within cells, or through cell layers or barriers are described. The compositions include a membrane barrier transport enhancing agent and are usually administered in combination with an enhancer and/or exposure to stimuli to effect disruption or altered permeability, transport or release. In a preferred embodiment, the compositions include compounds which disrupt endosomal membranes in response to the low pH in the endosomes but which are relatively inactive toward cell membranes, coupled directly or indirectly to a therapeutic or diagnostic agent. Other disruptive agents can also be used, responsive to stimuli and/or enhancers other than pH, such as light, electrical stimuli, electromagnetic stimuli, ultrasound, temperature, or combinations thereof.
    Type: Application
    Filed: February 7, 2014
    Publication date: August 7, 2014
    Applicants: UNIVERSITY OF MASSACHUSETTS, UNIVERSITY OF WASHINGTON
    Inventors: Allan S. Hoffman, Patrick Stayton, Oliver W. Press, Niren Murthy, Chantal Lackey Reed, Lawrence A. Crum, Pierre D. Mourad, Tyrone M. Porter, David Tirrell
  • Publication number: 20140127777
    Abstract: The present invention provides a method for producing modified stable polypeptides introducing at least one non-natural amino acid into the hydrophobic region of the polypeptide. The thermal and chemical stability of such polypeptides is improved compared to those properties of its corresponding wild type proteins. The invention further provides purified leucine zipper and coiled-coil proteins in which the leucine residues have been replaced with 5,5,5-trifluoroleucines, and the modified proteins so produced demonstrate increased thermal and chemical stability compared to their corresponding wild-type natural proteins.
    Type: Application
    Filed: October 29, 2013
    Publication date: May 8, 2014
    Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: David A. Tirrell, Yi Tang
  • Publication number: 20140045261
    Abstract: The invention provides certain embodiments relating to methods and compositions for incorporating non-natural amino acids into a polypeptide or protein by utilizing a mutant or modified aminoacyl-tRNA synthetase to charge the non-natural amino acid to a the corresponding tRNA. In certain embodiments, the tRNA is also modified such that the complex forms strict Watson-Crick base-pairing with a codon that normally forms wobble base-pairing with unmodified tRNA/aminoacyl-tRNA synthetase pairs.
    Type: Application
    Filed: August 16, 2013
    Publication date: February 13, 2014
    Applicant: California Institute of Technology
    Inventors: Pin Wang, Inchan Kwon, Soojin Son, Yi Tang, David Tirrell
  • Patent number: 8518666
    Abstract: The invention provides certain embodiments relating to methods and compositions for incorporating non-natural amino acids into a polypeptide or protein by utilizing a mutant or modified aminoacyl-tRNA synthetase to charge the non-natural amino acid to a the corresponding tRNA. In certain embodiments, the tRNA is also modified such that the complex forms strict Watson-Crick base-pairing with a codon that normally forms wobble base-pairing with unmodified tRNA/aminoacyl-tRNA synthetase pairs.
    Type: Grant
    Filed: March 5, 2007
    Date of Patent: August 27, 2013
    Assignee: California Institute of Technology
    Inventors: Pin Wang, Inchan Kwon, Soojin Son, Yi Tang, David Tirrell
  • Publication number: 20130012690
    Abstract: The present invention provides a method for producing modified stable polypeptides introducing at least one non-natural amino acid into the hydrophobic region of the polypeptide. The thermal and chemical stability of such polypeptides is improved compared to those properties of its corresponding wild type proteins. The invention further provides purified leucine zipper and coiled-coil proteins in which the leucine residues have been replaced with 5,5,5-trifluoroleucines, and the modified proteins so produced demonstrate increased thermal and chemical stability compared to their corresponding wild-type natural proteins.
    Type: Application
    Filed: January 20, 2012
    Publication date: January 10, 2013
    Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: David A. Tirrell, Yi Tang
  • Publication number: 20120231470
    Abstract: Methods and systems to identify compounds capable of altering a fatty acid elongation pathway and for identifying conditions under which fatty acids elongation can occur in a cell are described. The methods and systems comprise labeled fatty acid precursors and cells capable of elongating fatty acids. Methods for providing suitable components of an assay for identifying compounds capable of altering a fatty acid elongation pathway are described.
    Type: Application
    Filed: March 7, 2012
    Publication date: September 13, 2012
    Inventors: Janek SZYCHOWSKI, David A. TIRRELL, Sungjin Park
  • Patent number: 8236344
    Abstract: The present invention provides engineered proteins and biomedical products made from the engineered proteins. The biomedical products include lenses useful for ophthalmic purposes.
    Type: Grant
    Filed: June 4, 2007
    Date of Patent: August 7, 2012
    Assignee: California Institute of Technology
    Inventors: David A. Tirrell, Daniel M. Schwartz, Paul J. Nowatzki, Robert H. Grubbs