Abstract: The present invention provides engineered penicillin G acylase (PGA) enzymes having improved properties, polynucleotides encoding such enzymes, compositions including the enzymes, and methods of using the enzymes.

Abstract: Methods of characterizing an analyte using a detector such as a nanopore and an enzyme are provided. One aspect features methods for characterizing a double-stranded polynucleotide using a detector, e.g., without using a hairpin connecting a template and a complement of the double-stranded polynucleotide. Another aspect features methods for characterizing an analyte using a tag-modified nanopore with increased sensitivity and/or higher throughput. Compositions and systems including, e.g., adaptors for attachment to double-stranded polynucleotides and tag-modified nanopores, which can be used in the methods are also provided.

Abstract: A system for easily locating goods stored in a storage area including a computer coupled with a radio frequency transmitter or transceivers and goods placed in containers that are coupled to hangers that each has distinct electronic addresses. The hangers each have a hook that allows the hangers to be stored on rails coupled to racks in a storage area. The identifications of the goods and the electronic addresses for the corresponding hangers are associated with each other and stored on a computer database. When a user wants to pick up goods stored in the containers, the user inputs the identification for the goods and the computer will cause the radio frequency transmitter to emit a data packet that includes a search address and illumination data. The hangers each compare the search address to the electronic address and if there is a match, the hanger illuminates the light according to the illumination data.

Abstract: A system for easily locating goods stored in a storage area including a computer coupled with a radio frequency transmitter or transceivers and goods placed in containers that are coupled to hangers that each has distinct electronic addresses. The hangers each have a hook that allows the hangers to be stored on rails coupled to racks in a storage area. The identifications of the goods and the electronic addresses for the corresponding hangers are associated with each other and stored on a computer database. When a user wants to pick up goods stored in the containers, the user inputs the identification for the goods and the computer will cause the radio frequency transmitter to emit a data packet that includes a search address and illumination data. The hangers each compare the search address to the electronic address and if there is a match, the hanger illuminates the light according to the illumination data.