Abstract: A highly effective technique for fertilizing an oocyte with a sperm cell to produce a zygote can be carried out in a bioreactor and the zygote can optionally be cultured in the bioreactor into a blastocyst stage embryo for implantation into the uterus of a female recipient. The success rate and probability of fertilization is enhanced by operating the bioreactor reactor under conditions of inter-galactic motion which increases the probability of the sperm coming in close proximity to and fertilizing the oocyte. This method involves the steps of collecting an oocyte from a female donor, collecting sperm from a male donor, fertilizing the oocyte with the sperm in a rotating bioreactor to produce a zygote, culturing the zygote into a blastocyst stage embryo, and implanting the blastocyst stage embryo into a uterus of a recipient female.

Abstract: A highly effective technique for fertilizing an oocyte with a sperm cell to produce a zygote can be carried out in a bioreactor and the zygote can optionally be cultured in the bioreactor into a blastocyst stage embryo for implantation into the uterus of a female recipient. The success rate and probability of fertilization is enhanced by operating the bioreactor reactor under conditions of inter-galactic motion which increases the probability of the sperm coming in close proximity to and fertilizing the oocyte. This method involves the steps of collecting an oocyte from a female donor, collecting sperm from a male donor, fertilizing the oocyte with the sperm in a rotating bioreactor to produce a zygote, culturing the zygote into a blastocyst stage embryo, and implanting the blastocyst stage embryo into a uterus of a recipient female.

Abstract: A thermodynamic system includes a cyclical heat exchange system and a heat transfer system coupled to the cyclical heat exchange system to cool a portion of the cyclical heat exchange system. The heat transfer system includes an evaporator including a wall configured to be coupled to a portion of the cyclical heat exchange system and a primary wick coupled to the wall and a condenser coupled to the evaporator to form a closed loop that houses a working fluid.

Abstract: A bioreactor, kit, a method of using the device to promoting growing and/or culture of a cell, and a method for regenerating and/or improving the function of mammalian cells. The bioreactor includes a controlling circuit coupled to magnetic field emitter that emits relatively steep and short-lived magnetic field pulses during these an active ephemeral period. The bioreactor also provides a relatively long-term inactive phase in which no magnetic field pulses are imposed. The kit includes the unassembled components of the bioreactor. The method of using the bioreactor and of regenerating and/or improving the function of mammalian cells includes the step of applying a time variant magnetic field through the cell to promote growing and/or culturing, and then introducing the cells to a mammal to regenerate cells.

Abstract: An osteo or tissue healing kit and method of promoting the healing of compromised bone or tissue in a living mammal. The osteo or tissue healing device of the kit includes a magnetic field emitter and a controlling circuit, and at least one stem cell. The method of using the osteo or tissue healing kit includes placing at least one stem cell within the compromised bone or tissue and applying a time variant magnetic field.

Abstract: An osteo or tissue healing device, kit and method of using the device to promote healing of compromised bone or tissue in a living mammal is disclosed. The osteo or tissue healing device includes a magnetic field emitter and a controlling circuit. The magnetic field emitter of the device is electrically coupled to a controlling circuit in which the magnetic field emitter is configured to provide a time variant magnetic field when driven by an electric pulse train from the controlling circuit in such a manner that the time variant magnetic field results in a magnetic (B) field exhibiting a magnetic slew rate of at least about 10 kiloGauss/sec. The controlling circuit of the device is electrically coupled to the magnetic field emitter and is configured to be powered by a power source, in which the controlling circuit is configured to output the electric pulse train driving the magnetic field emitter.

Abstract: A method of extracting human progenitor cells from perivascular tissue of human umbilical cord. The extracted cells are then co-cultured with hematopoetic stem cells and are useful to grow and repair human tissues including bone. Also included are related methods and compositions related thereto.

Abstract: A cellular composition comprising a mesenchymal stem cell product and a biogel with methods for preparing and for using the composition. Tissue constructs made with the composition are also disclosed.

Abstract: A thermodynamic system includes a cyclical heat exchange system and a heat transfer system coupled to the cyclical heat exchange system to cool a portion of the cyclical heat exchange system. The heat transfer system includes an evaporator including a wall configured to be coupled to a portion of the cyclical heat exchange system and a primary wick coupled to the wall and a condenser coupled to the evaporator to form a closed loop that houses a working fluid.

Abstract: A heat transfer system includes an evaporator, a condenser having a vapor inlet and a liquid outlet, a vapor line providing fluid communication between a vapor outlet of the evaporator and the vapor inlet, and a liquid return line providing fluid communication between the liquid outlet and a liquid inlet entering the evaporator. The evaporator includes a heated wall, a liquid barrier wall containing working fluid, a primary wick positioned between the heated wall and the inner side of the liquid barrier wall, a vapor removal channel located at an interface between the primary wick and the heated wall, and a liquid flow channel located between the liquid barrier wall and the primary wick. The working fluid flows only along the inner side of the liquid barrier wall. The vapor removal channels extend to the vapor outlet and the liquid flow channel receives liquid from the liquid inlet.

Abstract: The present invention provides systems for growing two or three dimensional mammalian cells within a culture medium facilitated by an electromagnetic field, and preferably, a time varying electromagnetic field. The cells and culture medium are contained within a fixed or rotating culture vessel, and the electromagnetic field is emitted from at least one electrode. In one embodiment, the electrode is spaced from the vessel. The invention further provides methods to promote neural tissue regeneration by means of culturing the neural cells in the claimed system. In one embodiment, neuronal cells are grown within longitudinally extending tissue strands extending axially along and within electrodes comprising electrically conductive channels or guides through which a time varying electrical current is conducted, the conductive channels being positioned within a culture medium.

Abstract: A capillary wick for use in capillary evaporators has properties that prevent nucleation inside the body of the wick, resulting in suppression of back-conduction of heat from vapor channels to the liquid reservoir. Use of a central liquid flow channel in the wick is eliminated, and pore size in the wick is chosen to maximize available pressure for fluid pumping, while preventing nucleation in the wick body. The wick is embodied with different geometries, including cylindrical and flat. A flat capillary evaporator has substantially planar heat input surfaces for convenient mating to planar heat sources. The flat capillary evaporator is capable of being used with working fluids having high vapor pressures (i.e., greater that 10 psia). To contain the pressure of the vaporized working fluid, the opposed planar plates of the evaporator arc brazed or sintered to opposing sides of a metal wick. Additionally, a terrestrial loop heat pipe and a loop heat pipe having overall flat geometry are disclosed.

Abstract: The present invention provides systems for growing two or three dimensional mammalian cells within a culture medium facilitated by an electromagnetic field, and preferably, a time varying electromagnetic field. The cells and culture medium are contained within a fixed or rotating culture vessel, and the electromagnetic field is emitted from at least one electrode. In one embodiment, the electrode is spaced from the vessel. The invention further provides methods to promote neural tissue regeneration by means of culturing the neural cells in the claimed system. In one embodiment, neuronal cells are grown within longitudinally extending tissue strands extending axially along and within electrodes comprising electrically conductive channels or guides through which a time varying electrical current is conducted, the conductive channels being positioned within a culture medium.

Abstract: A capillary pump two phase heat transport system that combines the most favorable characteristics of a capillary pump loop (CPL) with the robustness and reliability of a loop heat pipe (LHP). Like a CPL, the hybrid loop has plural parallel evaporators, plural parallel condensers, and a back pressure flow regulator. Unlike CPLs, however, the hybrid system incorporates elements that form a secondary loop, which is essentially a LHP that is co-joined with a CPL to form an inseparable whole. Although secondary to the basic thermal management of the system thermal bus, the LHP secondary loop portion of the system provides for important operational functions that maintain healthy, robust and reliable operation. The LHP secondary loop portion provides a function of fluid management during start-up, steady state operation, and heat sink/heat source temperature and power cycling.

Abstract: The present invention provides systems for growing two or three dimensional mammalian cells within a culture medium facilitated by an electromagnetic field, and preferably, a time varying electromagnetic field. The cells and culture medium are contained within a fixed or rotating culture vessel, and the electromagnetic field is emitted from at least one electrode. In one embodiment, the electrode is spaced from the vessel. The invention further provides methods to promote neural tissue regeneration by means of culturing the neural cells in the claimed system. In one embodiment, neuronal cells are grown within longitudinally extending tissue strands extending axially along and within electrodes comprising electrically conductive channels or guides through which a time varying electrical current is conducted, the conductive channels being positioned within a culture medium.

Abstract: A process for propagating a pathogen in a three-dimensional tissue mass cultured at microgravity conditions in a culture vessel containing culture media and a culture matrix is provided. The three-dimensional tissue mass is inoculated with a pathogen and pathogen replication in the cells of the tissue mass achieved.

Abstract: Normal mammalian tissue and the culturing process has been developed for the three groups of organ, structural and blood tissue. The cells are grown in vitro under micro-gravity culture conditions and form three dimensional cells aggregates with normal cell function. The microgravity culture conditions may be microgravity or simulated micro-gravity created in a horizontal rotating wall culture vessel. The medium used for culturing the cells, especially a mixture of epithelial and mesenchymal cells contains a mixture of Mem-alpha and Leibovits L15 supplemented with glucose, galactose and fructose.

Abstract: Artificial high-fidelity three-dimensional human urogenital tract carcinomas are propagated under in vitro-microgravity conditions from carcinoma cells. Artificial high-fidelity three-dimensional human urogenital tract carcinomas are also propagated from a coculture of normal urogenital tract cells inoculated with carcinoma cells. The microgravity culture conditions may be microgravity or simulated microgravity created in a horizontal rotating wall culture vessel.

Abstract: The process of the present invention relates to a three dimensional co-culture process.

Abstract: Normal mammalian tissue and the culturing process has been developed for the three groups of organ, structural and blood tissue. The cells are grown in vitro under microgravity culture conditions and form three dimensional cells aggregates with normal cell function. The microgravity culture conditions may be microgravity or simulated microgravity created in a horizontal rotating wall culture vessel.