Patents by Inventor David Alland
David Alland has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11834721Abstract: This invention relates to nucleic acids, reagents and methods for detecting Rifampicin-resistant Mycobacterium tuberculosis in a sample from a subject. In one aspect, the invention provides methods of detecting Rifampicin resistant M. tuberculosis in a sample comprising (a) amplifying a nucleic acid containing the rifampicin resistance determining region (RRDR) of the rpoB gene in a sample to provide an amplified nucleic acid; (b) probing the amplified nucleic acid with at least three molecular beacon probes for an RRDR mutant target; (c) conducting melting temperature (Tm) analysis to determine a Tm value for each probe; and (d) comparing the Tm value for each probe with a Tm value for a wild-type RRDR region, wherein a Tm value for at least one of the molecular beacon probes that is greater than the Tm value for the wild-type RRDR indicates the presence of rifampicin resistant M. tuberculosis in the sample.Type: GrantFiled: May 14, 2020Date of Patent: December 5, 2023Assignee: RUTGERS, THE STATE UNIVERSITY OF NEW JERSEYInventors: David Alland, Soumitesh Chakravorty
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Publication number: 20230295749Abstract: This disclosure provides an improved assay capable of identifying and distinguishing viral variants, such as SARS-CoV-2 variants and subvariants, including Alpha, Beta, Gamma, Delta, and Omicron, with high specificity and sensitivity. The disclosed assay can be used for real-time monitoring of viral variants (e.g., SARS-CoV-2 variants) spread without the need for whole-genome sequencing on all samples.Type: ApplicationFiled: January 13, 2023Publication date: September 21, 2023Applicant: Rutgers, The State University of New JerseyInventors: David Alland, Padmapriya P. Banada, Salvatore A.E. Marras
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Publication number: 20220144781Abstract: The invention provides compounds of formula I: and salts thereof wherein R1-R5 have any of the meanings described in the specification. The compounds are useful for treating bacterial infections (e.g. tuberculosis).Type: ApplicationFiled: November 2, 2021Publication date: May 12, 2022Applicant: RUTGERS, THE STATE UNIVERSITY OF NEW JERSEYInventors: Joel S. FREUNDLICH, David ALLAND, Matthew B. NEIDITCH, Pradeep KUMAR, Glenn CAPODAGLI, Divya AWASTHI, Sean EKINS
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Publication number: 20220088271Abstract: The present invention is a method for producing allograft tissue by applying an antimicrobial solution to allograft tissue. The antimicrobial solution exhibits antimicrobial activity to make allograft resistant to microbial organisms, such as a bacterium.Type: ApplicationFiled: December 2, 2021Publication date: March 24, 2022Applicant: Rutgers, The State University of New JerseyInventors: Sheldon S. Lin, Joseph Benevenia, Michael J. Vives, Pradeep Kumar, David Alland, James Patrick O'Connor
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Publication number: 20220064715Abstract: The present invention relates to novel primers and sloppy molecular beacon and molecular beacon probes for amplifying segments from different genes in Mycobacterium tuberculosis for identifying the presence of M.tb DNA and/or resistance to anti-tuberculosis drugs.Type: ApplicationFiled: November 16, 2021Publication date: March 3, 2022Applicant: Rutgers, The State University of New JerseyInventors: David Alland, Soumitesh Chakravorty
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Patent number: 11186549Abstract: The invention provides compounds of formula (I): and salts thereof wherein R1-R5 have any of the meanings described in the specification. The compounds are useful for treating bacterial infections (e.g. tuberculosis).Type: GrantFiled: August 29, 2018Date of Patent: November 30, 2021Assignee: RUTGERS, THE STATE UNIVERSITY OF NEW JERSEYInventors: Joel S. Freundlich, David Alland, Matthew B. Neiditch, Pradeep Kumar, Glenn Capodagli, Divya Awasthi, Sean Ekins
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Patent number: 11180816Abstract: The present invention relates to novel primers and sloppy molecular beacon and molecular beacon probes for amplifying segments from different genes in Mycobacterium tuberculosis for identifying the presence of M.tb DNA and/or resistance to anti-tuberculosis drugs.Type: GrantFiled: October 9, 2015Date of Patent: November 23, 2021Assignee: RUTGERS, THE STATE UNIVERSITY OF NEW JERSEYInventors: David Alland, Soumitesh Chakravorty
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Publication number: 20200347439Abstract: This invention relates to nucleic acids, reagents and methods for detecting Rifampicin-resistant Mycobacterium tuberculosis in a sample from a subject. In one aspect, the invention provides methods of detecting Rifampicin resistant M. tuberculosis in a sample comprising (a) amplifying a nucleic acid containing the rifampicin resistance determining region (RRDR) of the rpoB gene in a sample to provide an amplified nucleic acid; (b) probing the amplified nucleic acid with at least three molecular beacon probes for an RRDR mutant target; (c) conducting melting temperature (Tm) analysis to determine a Tm value for each probe; and (d) comparing the Tm value for each probe with a Tm value for a wild-type RRDR region, wherein a Tm value for at least one of the molecular beacon probes that is greater than the Tm value for the wild-type RRDR indicates the presence of rifampicin resistant M. tuberculosis in the sample.Type: ApplicationFiled: May 14, 2020Publication date: November 5, 2020Inventors: David Alland, Soumitesh Chakravorty
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Publication number: 20200199076Abstract: The invention provides compounds of formula (I): and salts thereof wherein R1-R5 have any of the meanings described in the specification. The compounds are useful for treating bacterial infections (e.g. tuberculosis).Type: ApplicationFiled: August 29, 2018Publication date: June 25, 2020Applicant: RUTGERS, THE STATE UNIVERSITY OF NEW JERSEYInventors: Joel S. FREUNDLICH, David ALLAND, Matthew B. NEIDITCH, Pradeep KUMAR, Glenn CAPODAGLI, Divya AWASTHI, Sean EKINS
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Patent number: 10655189Abstract: This invention relates to nucleic acids, reagents and methods for detecting Rifampicin-resistant Mycobacterium tuberculosis in a sample from a subject. In one aspect, the invention provides methods of detecting Rifampicin resistant M. tuberculosis in a sample comprising (a) amplifying a nucleic acid containing the rifampicin resistance determining region (RRDR) of the rpoB gene in a sample to provide an amplified nucleic acid; (b) probing the amplified nucleic acid with at least three molecular beacon probes for an RRDR mutant target; (c) conducting melting temperature (Tm) analysis to determine a Tm value for each probe; and (d) comparing the Tm value for each probe with a Tm value for a wild-type RRDR region, wherein a Tm value for at least one of the molecular beacon probes that is greater than the Tm value for the wild-type RRDR indicates the presence of rifampicin resistant M. tuberculosis in the sample.Type: GrantFiled: July 14, 2017Date of Patent: May 19, 2020Assignee: RUTGERS, THE STATE UNIVERSITY OF NEW JERSEYInventors: David Alland, Soumitesh Chakravorty
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Publication number: 20170321258Abstract: This invention relates to nucleic acids, reagents and methods for detecting Rifampicin-resistant Mycobacterium tuberculosis in a sample from a subject. In one aspect, the invention provides methods of detecting Rifampicin resistant M. tuberculosis in a sample comprising (a) amplifying a nucleic acid containing the rifampicin resistance determining region (RRDR) of the rpoB gene in a sample to provide an amplified nucleic acid; (b) probing the amplified nucleic acid with at least three molecular beacon probes for an RRDR mutant target; (c) conducting melting temperature (Tm) analysis to determine a Tm value for each probe; and (d) comparing the Tm value for each probe with a Tm value for a wild-type RRDR region, wherein a Tm value for at least one of the molecular beacon probes that is greater than the Tm value for the wild-type RRDR indicates the presence of rifampicin resistant M. tuberculosis in the sample.Type: ApplicationFiled: July 14, 2017Publication date: November 9, 2017Applicant: Rutgers, The State University of New JerseyInventors: David Alland, Soumitesh Chakravorty
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Publication number: 20170247747Abstract: The present invention relates to novel primers and sloppy molecular beacon and molecular beacon probes for amplifying segments from different genes in Mycobacterium tuberculosis for identifying the presence of M.tb DNA and/or resistance to anti-tuberculosis drugs.Type: ApplicationFiled: October 9, 2015Publication date: August 31, 2017Inventors: David Alland, Soumitesh Chakravorty
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Patent number: 9708671Abstract: This invention relates to nucleic acids, reagents and methods for detecting Rifampicin-resistant Mycobacterium tuberculosis in a sample from a subject. In one aspect, the invention provides methods of detecting Rifampicin resistant M. tuberculosis in a sample comprising (a) amplifying a nucleic acid containing the rifampicin resistance determining region (RRDR) of the rpoB gene in a sample to provide an amplified nucleic acid; (b) probing the amplified nucleic acid with at least three molecular beacon probes for an RRDR mutant target; (c) conducting melting temperature (Tm) analysis to determine a Tm value for each probe; and (d) comparing the Tm value for each probe with a Tm value for a wild-type RRDR region, wherein a Tm value for at least one of the molecular beacon probes that is greater than the Tm value for the wildtype RRDR indicates the presence of rifampicin resistant M. tuberculosis in the sample.Type: GrantFiled: April 10, 2013Date of Patent: July 18, 2017Assignee: RUTGERS, THE STATE UNIVERSITY OF NEW JERSEYInventors: David Alland, Soumitesh Chakravorty
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Patent number: 9260761Abstract: The invention provides assays that can detect multiple genetic variants of a gene (e.g., a mycobacterium gene) in a sample using a pool (using 2, 3, 4, or more) of oligonucletide hybridization probes.Type: GrantFiled: November 9, 2009Date of Patent: February 16, 2016Assignee: PHRI Properties, Inc.Inventors: Sanjay Tyagi, Fred Kramer, David Alland
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Publication number: 20150079589Abstract: This invention relates to nucleic acids, reagents and methods for detecting Rifampicin-resistant Mycobacterium tuberculosis in a sample from a subject. In one aspect, the invention provides methods of detecting Rifampicin resistant M. tuberculosis in a sample comprising (a) amplifying a nucleic acid containing the rifampicin resistance determining region (RRDR) of the rpoB gene in a sample to provide an amplified nucleic acid; (b) probing the amplified nucleic acid with at least three molecular beacon probes for an RRDR mutant target; (c) conducting melting temperature (Tm) analysis to determine a Tm value for each probe; and (d) comparing the Tm value for each probe with a Tm value for a wild-type RRDR region, wherein a Tm value for at least one of the molecular beacon probes that is greater than the Tm value for the wildtype RRDR indicates the presence of rifampicin resistant M. tuberculosis in the sample.Type: ApplicationFiled: April 10, 2013Publication date: March 19, 2015Applicant: Rutgers, The State University of New JerseyInventors: David Alland, Soumitesh Chakravorty
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Publication number: 20100279294Abstract: A method for detecting and genotyping Escherichia coli O157:H7 strains, including detecting nucleotides at single nucleotide polymorphism (SNP) loci, the identity of which nucleotides define SNP genotypes. A method for genotyping E. coli O157:H7 strains, including detecting thirty-two nucleotides at thirty-two single nucleotide polymorphism (SNP) loci, the identity of which nucleotides define thirty-six SNP genotypes. Multiplexed primer trios capable of detecting the nucleotides at E. coli SNP loci, and a kit including one or more primer trios.Type: ApplicationFiled: March 5, 2010Publication date: November 4, 2010Inventors: THOMAS WHITTAM, Shannon Manning, David Alland, Alifiya Motiwala
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Publication number: 20100120048Abstract: The invention provides assays that can detect multiple genetic variants of a gene (e.g., a mycobacterium gene) in a sample using a pool (using 2, 3, 4, or more) of oligonucletide hybridization probes.Type: ApplicationFiled: November 9, 2009Publication date: May 13, 2010Applicant: The Public Health Research Institute of the City of New York, Inc.Inventors: Sanjay Tyagi, Fred Kramer, David Alland
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Patent number: 7662550Abstract: The invention provides assays that can detect multiple genetic variants of a gene (e.g., a mycobacterial gene) in a sample using a pool (e.g., 2, 3, 4, or more) of oligonucleotide hybridization probes.Type: GrantFiled: October 13, 2000Date of Patent: February 16, 2010Assignee: PHRI Properties, Inc.Inventors: Sanjay Tyagi, Fred R. Kramer, David Alland
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Publication number: 20060121487Abstract: Method and kits for detecting a single nucleotide polymorphism in an organism via hairpin shaped primers that discriminate between different alleles by situating the 3? nucleotide at the location of a single nucleotide polymorphism are provided.Type: ApplicationFiled: December 24, 2003Publication date: June 8, 2006Inventors: David Alland, Manzour Hazbon
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Patent number: 6821769Abstract: This invention relates to the identification, cloning, sequencing and characterization of the iniB, iniA and iniC genes of mycobacteria which are induced by a broad class of antibiotics that act by inhibiting cell wall biosynthesis, including the first line antituberculosis agents, isoniazid and ethambutol. The present invention provides purified and isolated iniB, iniA, iniC and iniB promoter nucleic acids which may comprise the iniBAC operon, as well as mutated forms of these nucleic acids. The present invention also provides one or more single-stranded nucleic acid probes which specifically hybridize to the iniB, iniA, iniC and iniB promoter nucleic acids, and mixtures thereof, which may be formulated in kits, and used in the diagnosis of drug-resistant mycobacterial strain. The present invention also provides methods for the screening and identification of drugs effective against Mycobacterium tuberculosis using induction of the iniB promoter.Type: GrantFiled: July 31, 2001Date of Patent: November 23, 2004Assignee: Albert Einstein College of Medicine of Yeshiva UniversityInventors: David Alland, Barry R. Bloom, William R. Jacobs, Jr.