Abstract: Methods of isolating clinical-grade plasmid DNA from manufacturing processes, including large-scale fermentation regimes, are disclosed which encompass alternatives to two core unit operations common to plasmid DNA purification processes. The novel upstream and downstream purification processes disclosed herein provide for reduced production costs and increase process robustness. Either or both of the purification processes disclosed herein may be used in combination with additional purification steps known in the art that are associated with DNA plasmid purification technology.

Abstract: Methods of isolating clinical-grade plasmid DNA from manufacturing processes, including large-scale fermentation regimes, are disclosed which encompass alternatives to two core unit operations common to plasmid DNA purification processes. The novel upstream and downstream purification processes disclosed herein provide for reduced production costs and increase process robustness. Either or both of the purification processes disclosed herein may be used in combination with additional purification steps known in the art that are associated with DNA plasmid purification technology.

Abstract: A multiple-ply web registration apparatus includes a frame through which a pair of webs enter in side-by-side relation and exit in juxtaposed registered relation. A pair of turning bars are mounted within the frame and oriented obliquely with respect to the direction of travel of the webs. The first web is wrapped around the turning bars to laterally shift the web over the top of the second web. At least one of the turning bars is adjustable towards and away from the other turning bar so as to permit adjustment of the lateral shifting of the first web with respect to the second web. The second web extends around an idler roller within the frame, which is adjustable so that the distance traveled by the second web is identical to the distance traveled by the first web.