Patents by Inventor David B. Kolesky
David B. Kolesky has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11648106Abstract: This disclosure features artificial tympanic membrane graft devices and two-component bilayer graft devices that include a scaffold having a plurality of ribs made of a first material and a plurality of spaces between the ribs filled or made with the first material, a different, second material, a combination of the first and a second materials, or a combination of a second material and one or more other different materials. The bilayer graft devices have two components or layers. One component, e.g., the underlay graft device, can include a projection, and the second component, e.g., the overlay graft device, can include an opening that corresponds to the projection (or vice versa) so that the opening and the projection can secure the two layers together in a “lock and key” manner. This disclosure also features methods of making, using, and implanting the three-dimensional artificial tympanic membrane and bilayer graft devices.Type: GrantFiled: September 22, 2020Date of Patent: May 16, 2023Assignees: Massachusetts Eye and Ear Infirmary, President and Fellows of Harvard CollegeInventors: Aaron K. Remenschneider, Elliott Kozin, Nicole Leah Black, Michael J. McKenna, Daniel J. Lee, Jennifer A. Lewis, John Rosowski, David B. Kolesky, Mark A. Skylar-Scott, Alexander D. Valentine
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Patent number: 11559607Abstract: Described are devices and methods for use in connection with organ replacement or organ assist therapy in a patient.Type: GrantFiled: September 6, 2017Date of Patent: January 24, 2023Assignee: PRESIDENT AND FELLOWS OF HARVARD COLLEGEInventors: David B. Kolesky, Kimberly A. Homan, Jennifer A. Lewis, Yen-Chih Lin
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Patent number: 11214768Abstract: Methods of tissue engineering, and more particularly methods and compositions for generating various vascularized 3D tissues, such as 3D vascularized embryoid bodies and organoids are described. Certain embodiments relate to a method of generating functional human tissue, the method comprising embedding an embryoid body or organoid in a tissue construct comprising a first vascular network and a second vascular network, each vascular network comprising one or more interconnected vascular channels; exposing the embryoid body or organoid to one or more biological agents, a biological agent gradient, a pressure, and/or an oxygen tension gradient, thereby inducing angiogenesis of capillary vessels to and/or from the embryoid body or organoid; and vascularizing the embryoid body or organoid, the capillary vessels connecting the first vascular network to the second vascular network, thereby creating a single vascular network and a perfusable tissue structure.Type: GrantFiled: March 3, 2016Date of Patent: January 4, 2022Assignee: PRESIDENT AND FELLOWS OF HARVARD COLLEGEInventors: Jennifer A. Lewis, Mark A. Skylar-Scott, David B. Kolesky, Kimberly A. Homan, Alex H. M. Ng, George M. Church
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Publication number: 20210000590Abstract: This disclosure features artificial tympanic membrane graft devices and two-component bilayer graft devices that include a scaffold having a plurality of ribs made of a first material and a plurality of spaces between the ribs filled or made with the first material, a different, second material, a combination of the first and a second materials, or a combination of a second material and one or more other different materials. The bilayer graft devices have two components or layers. One component, e.g., the underlay graft device, can include a projection, and the second component, e.g., the overlay graft device, can include an opening that corresponds to the projection (or vice versa) so that the opening and the projection can secure the two layers together in a “lock and key” manner. This disclosure also features methods of making, using, and implanting the three-dimensional artificial tympanic membrane and bilayer graft devices.Type: ApplicationFiled: September 22, 2020Publication date: January 7, 2021Inventors: Aaron K. Remenschneider, Elliott Kozin, Nicole Leah Black, Michael J. McKenna, Daniel J. Lee, Jennifer A. Lewis, John Rosowski, David B. Kolesky, Mark A. Skylar-Scott, Alexander D. Valentine
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Publication number: 20200360567Abstract: A 3D printed tubular construct, such as a nephron, with or without embedded vasculature as well as methods of printing tubular tissue constructs are described.Type: ApplicationFiled: May 29, 2020Publication date: November 19, 2020Applicant: President and Fellows of Harvard CollegeInventors: Jennifer A. Lewis, Kimberly A. Homan, David B. Kolesky, Ryan L. Truby, Mark A. Skylar-Scott
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Publication number: 20200248147Abstract: Described are methods of enhancing development of renal organoids, methods of using the same, and kits.Type: ApplicationFiled: June 8, 2018Publication date: August 6, 2020Applicants: President and Fellows of Harvard College, The Brigham And Women's Hospital, Inc.Inventors: Kimberly A. Homan, Navin R. Gupta, Katharina T. Kroll, David B. Kolesky, Mark Skylar-Scott, Michael T. Valerius, Joseph Bonventre, Ryuji Morizane, Jennifer Lewis
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Patent number: 10702630Abstract: A 3D printed tubular construct, such as a nephron, with or without embedded vasculature as well as methods of printing tubular tissue constructs are described.Type: GrantFiled: May 4, 2016Date of Patent: July 7, 2020Assignee: PRESIDENT AND FELLOWS OF HARVARD COLLEGEInventors: Jennifer A. Lewis, Kimberly A. Homan, David B. Kolesky, Ryan L. Truby, Mark A. Skylar-Scott
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Publication number: 20200164109Abstract: Described are methods for producing multi-layered tubular tissue structures, tissue structures produced by the methods, and their use.Type: ApplicationFiled: July 20, 2018Publication date: May 28, 2020Applicant: President and Fellows of Harvard CollegeInventors: Katharina Theresa Kroll, Kimberly A. Homan, Mark A. Skylar-Scott, Sebastien G.M. Uzel, David B. Kolesky, Patrick Lustenberger, Jennifer A. Lewis
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Patent number: 10612986Abstract: A printed stretchable strain sensor comprises a seamless elastomeric body and a strain-sensitive conductive structure embedded in the seamless elastomeric body. The strain-sensitive conductive structure comprises one or more conductive filaments arranged in a continuous pattern. A method of printing a stretchable strain sensor comprises depositing one or more conductive filaments in a predetermined continuous pattern into or onto a support matrix. After the depositing, the support matrix is cured to embed a strain-sensitive conductive structure in a seamless elastomeric body.Type: GrantFiled: October 11, 2018Date of Patent: April 7, 2020Assignee: President and Fellows of Harvard CollegeInventors: Jennifer A. Lewis, Joseph T. Muth, Daniel M. Vogt, Ryan L. Truby, Yigit Menguc, David B. Kolesky, Robert J. Wood
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Publication number: 20190224370Abstract: Described are devices and methods for use in connection with organ replacement or organ assist therapy in a patient.Type: ApplicationFiled: September 6, 2017Publication date: July 25, 2019Applicant: President and Fellows of Harvard CollegeInventors: David B. KOLESKY, Kimberly A. HOMAN, Jennifer A. LEWIS, Yen-Chih LIN
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Publication number: 20190094089Abstract: A printed stretchable strain sensor comprises a seamless elastomeric body and a strain-sensitive conductive structure embedded in the seamless elastomeric body. The strain-sensitive conductive structure comprises one or more conductive filaments arranged in a continuous pattern. A method of printing a stretchable strain sensor comprises depositing one or more conductive filaments in a predetermined continuous pattern into or onto a support matrix. After the depositing, the support matrix is cured to embed a strain-sensitive conductive structure in a seamless elastomeric body.Type: ApplicationFiled: October 11, 2018Publication date: March 28, 2019Applicant: President and Fellows of Harvard CollegeInventors: Jennifer A. Lewis, Joseph T. Muth, Daniel M. Vogt, Ryan L. Truby, Yigit Menguc, David B. Kolesky, Robert J. Wood
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Publication number: 20190022283Abstract: A printed tissue construct comprises one or more tissue patterns, where each tissue pattern comprises a plurality of viable cells of one or more predetermined cell types. A network of vascular channels interpenetrates the one or more tissue patterns. An extracellular matrix composition at least partially surrounds the one or more tissue patterns and the network of vascular channels. A method of printing a tissue construct with embedded vasculature comprises depositing one or more cell-laden filaments, each comprising a plurality of viable cells, on a substrate to form one or more tissue patterns. Each of the one or more tissue patterns comprises one or more predetermined cell types. One or more sacrificial filaments, each comprising a fugitive ink, are deposited on the substrate to form a vascular pattern interpenetrating the one or more tissue patterns. The vascular pattern and the one or more tissue patterns are at least partially surrounded with an extracellular matrix composition.Type: ApplicationFiled: September 26, 2018Publication date: January 24, 2019Applicant: President and Fellows of Harvard CollegeInventors: Jennifer A. LEWIS, David B. KOLESKY, Mark A. SKYLAR-SCOTT, Kimberly A. HOMAN, Ryan L. TRUBY, Amelia Sydney GLADMAN
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Patent number: 10151649Abstract: A printed stretchable strain sensor comprises a seamless elastomeric body and a strain-sensitive conductive structure embedded in the seamless elastomeric body. The strain-sensitive conductive structure comprises one or more conductive filaments arranged in a continuous pattern. A method of printing a stretchable strain sensor comprises depositing one or more conductive filaments in a predetermined continuous pattern into or onto a support matrix. After the depositing, the support matrix is cured to embed a strain-sensitive conductive structure in a seamless elastomeric body.Type: GrantFiled: November 17, 2014Date of Patent: December 11, 2018Assignee: PRESIDENT AND FELLOWS OF HARVARD COLLEGEInventors: Jennifer A. Lewis, Joseph T. Muth, Daniel M. Vogt, Ryan L. Truby, Yigit Menguc, David B. Kolesky, Robert J. Wood
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Patent number: 10117968Abstract: A printed tissue construct comprises one or more tissue patterns, where each tissue pattern comprises a plurality of viable cells of one or more predetermined cell types. A network of vascular channels interpenetrates the one or more tissue patterns. An extracellular matrix composition at least partially surrounds the one or more tissue patterns and the network of vascular channels. A method of printing a tissue construct with embedded vasculature comprises depositing one or more cell-laden filaments, each comprising a plurality of viable cells, on a substrate to form one or more tissue patterns. Each of the one or more tissue patterns comprises one or more predetermined cell types. One or more sacrificial filaments, each comprising a fugitive ink, are deposited on the substrate to form a vascular pattern interpenetrating the one or more tissue patterns. The vascular pattern and the one or more tissue patterns are at least partially surrounded with an extracellular matrix composition.Type: GrantFiled: November 4, 2014Date of Patent: November 6, 2018Assignee: President And Fellows Of Harvard CollegeInventors: Jennifer A. Lewis, David B. Kolesky, Mark A. Skylar-Scott, Kimberly A. Homan, Ryan L. Truby, Amelia Sydney Gladman
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Publication number: 20180110901Abstract: A 3D printed tubular construct, such as a nephron, with or without embedded vasculature as well as methods of printing tubular tissue constructs are described.Type: ApplicationFiled: May 4, 2016Publication date: April 26, 2018Applicant: President and Fellows of Harvard CollegeInventors: Jennifer A. Lewis, Kimberly A. Homan, David B. Kolesky, Ryan L. Truby, Mark A. Skylar-Scott
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Publication number: 20180030409Abstract: Methods of tissue engineering, and more particularly methods and compositions for generating various vascularized 3D tissues, such as 3D vascularized embryoid bodies and organoids are described. Certain embodiments relate to a method of generating functional human tissue, the method comprising embedding an embryoid body or organoid in a tissue construct comprising a first vascular network and a second vascular network, each vascular network comprising one or more interconnected vascular channels; exposing the embryoid body or organoid to one or more biological agents, a biological agent gradient, a pressure, and/or an oxygen tension gradient, thereby inducing angiogenesis of capillary vessels to and/or from the embryoid body or organoid; and vascularizing the embryoid body or organoid, the capillary vessels connecting the first vascular network to the second vascular network, thereby creating a single vascular network and a perfusable tissue structure.Type: ApplicationFiled: March 3, 2016Publication date: February 1, 2018Inventors: Jennifer A. Lewis, Mark A. Skylar-Scott, David B. Kolesky, Kimberly A. Homan, Alex H.M. Ng, George M. Church
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Publication number: 20170203566Abstract: A multinozzle deposition system for direct write applications comprises a body including a first network of microchannels embedded therein, where the first network of microchannels extends from a parent microchannel through a series of furcations to a plurality of branching microchannels. The series consists of k generations with furcation number m where the kth generation includes mk branching microchannels. A first end of the body includes a single inlet to the parent microchannel and a second end of the body includes mk outlets from the branching microchannels, where k is an integer greater than or equal to 1 and m is an integer greater than or equal to 2. The body comprises a material having a sufficient rigidity to sustain a pressure in the microchannels of about 690 kPa or greater without distortion.Type: ApplicationFiled: April 5, 2017Publication date: July 20, 2017Applicant: The Board of Trustees of the University of IllinoisInventors: Jennifer A. Lewis, Christopher J. Hansen, Steven Kranz, John J. Vericella, Willie Wu, David B. Kolesky
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Patent number: 9643358Abstract: A multinozzle deposition system for direct write applications comprises a body including a first network of microchannels embedded therein, where the first network of microchannels extends from a parent microchannel through a series of furcations to a plurality of branching microchannels. The series consists of k generations with furcation number m where the kth generation includes mk branching microchannels. A first end of the body includes a single inlet to the parent microchannel and a second end of the body includes mk outlets from the branching microchannels, where k is an integer greater than or equal to 1 and m is an integer greater than or equal to 2. The body comprises a material having a sufficient rigidity to sustain a pressure in the microchannels of about 690 kPa or greater without distortion.Type: GrantFiled: June 29, 2012Date of Patent: May 9, 2017Assignee: The Board of Trustees of The University of IllinoisInventors: Jennifer A. Lewis, Christopher J. Hansen, Steven Kranz, John J. Vericella, Willie Wu, David B. Kolesky
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Publication number: 20160287756Abstract: A printed tissue construct comprises one or more tissue patterns, where each tissue pattern comprises a plurality of viable cells of one or more predetermined cell types. A network of vascular channels interpenetrates the one or more tissue patterns. An extracellular matrix composition at least partially surrounds the one or more tissue patterns and the network of vascular channels. A method of printing a tissue construct with embedded vasculature comprises depositing one or more cell-laden filaments, each comprising a plurality of viable cells, on a substrate to form one or more tissue patterns. Each of the one or more tissue patterns comprises one or more predetermined cell types. One or more sacrificial filaments, each comprising a fugitive ink, are deposited on the substrate to form a vascular pattern interpenetrating the one or more tissue patterns. The vascular pattern and the one or more tissue patterns are at least partially surrounded with an extracellular matrix composition.Type: ApplicationFiled: November 4, 2014Publication date: October 6, 2016Applicant: President and Fellows of Harvard CollegeInventors: Jennifer A. Lewis, David B. Kolesky, Mark A. Skylar-Scott, Kimberly A. Homan, Ryan L. Truby, Amelia Sydney Gladman
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Publication number: 20160290880Abstract: A printed stretchable strain sensor comprises a seamless elastomeric body and a strain-sensitive conductive structure embedded in the seamless elastomeric body. The strain-sensitive conductive structure comprises one or more conductive filaments arranged in a continuous pattern. A method of printing a stretchable strain sensor comprises depositing one or more conductive filaments in a predetermined continuous pattern into or onto a support matrix. After the depositing, the support matrix is cured to embed a strain-sensitive conductive structure in a seamless elastomeric body.Type: ApplicationFiled: November 17, 2014Publication date: October 6, 2016Applicant: President and Fellows of Harvard CollegeInventors: Jennifer A. Lewis, Joseph T. Muth, Daniel M. Vogt, Ryan L. Truby, Yigit Menguc, David B. Kolesky, Robert J. Wood