Patents by Inventor David B. Rozema

David B. Rozema has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Reversible modification of membrane interaction
    Publication number: 20030220264
    Abstract: An process for the reversible modification of membrane interaction of a compound is described. Modification of membrane interaction can be used to facilitate delivery of molecules to cells in vitro and in vivo. The described modifiers, which are used to reversibly inactivate the membrane active compounds, can also be utilized as cross-linkers or to reverse the charge of a molecule.
    Type: Application
    Filed: May 23, 2003
    Publication date: November 27, 2003
    Inventors: David B. Rozema, Darren Wakefield, Jon A. Wolff, Kirk Ekena, James E. Hagstrom
  • Intravascular delivery of non-viral nucleic acid
    Publication number: 20030216347
    Abstract: Disclosed is a process for transfecting genetic material into a mammalian cell to alter endogenous properties of the cell. The process comprises designing a polynucleotide for transfection. Then the polynucleotide is inserted into a mammalian vessel such as a tail vein or artery. Prior to insertion, subsequent to insertion, or concurrent with insertion the permeability of the vessel is increased thereby the genetic material is delivered to the parenchymal cell altering endogenous properties of the cell.
    Type: Application
    Filed: June 20, 2003
    Publication date: November 20, 2003
    Inventors: Sean D. Monahan, Jon A. Wolff, Paul M. Slattum, James E. Hagstrom, Vladimir G. Budker, David B. Rozema
  • Compositions and methods for drug delivery using pH sensitive molecules
    Publication number: 20030199090
    Abstract: A system relating to the delivery of desired compounds (e.g., drugs and nucleic acids) into cells using pH-sensitive delivery systems. The system provides compositions and methods for the delivery and release of a compound to a cell.
    Type: Application
    Filed: February 26, 2002
    Publication date: October 23, 2003
    Inventors: Sean D. Monahan, Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, David B. Rozema
  • Compositions and methods for drug delivery using pH sensitive molecules
    Patent number: 6630351
    Abstract: A system relating to the delivery of desired compounds (e.g., drugs and nucleic acids) into cells using pH-sensitive delivery systems. The system provides compositions and methods for the delivery and release of a compound to a cell.
    Type: Grant
    Filed: June 7, 2000
    Date of Patent: October 7, 2003
    Assignee: Mirus Corporation
    Inventors: Sean D. Monahan, Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, David B. Rozema
  • Intravascular delivery of non-viral nucleic acid
    Patent number: 6627616
    Abstract: Disclosed is a process for transfecting genetic material into a mammalian cell to alter endogenous properties of the cell. The process comprises designing a polynucleotide for transfection. Then the polynucleotide is inserted into a mammalian vessel such as a tail vein or artery. Prior to insertion, subsequent to insertion, or concurrent with insertion the permeability of the vessel is increased thereby the genetic material is delivered to the parenchymal cell altering endogenous properties of the cell.
    Type: Grant
    Filed: November 23, 1999
    Date of Patent: September 30, 2003
    Assignee: Mirus Corporation
    Inventors: Sean D. Monahan, Jon A. Wolff, Paul M. Slattum, James E. Hagstrom, Vladimir G. Budker, David B. Rozema
  • Polynucleotide complex delivery
    Publication number: 20030166280
    Abstract: Disclosed is a complex for providing nucleic acid expression in a cell. A polynucleotide and a polymer are mixed together to form the complex wherein the zeta potential of the complex is not positive. Then the complex is delivered to the cell wherein the nucleic acid is expressed.
    Type: Application
    Filed: February 27, 2002
    Publication date: September 4, 2003
    Inventors: Sean D. Monahan, Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, David B. Rozema, Paul M. Slattum
  • Expression of zeta negative and zeta positive nucleic acids using a dystrophin gene
    Publication number: 20030130224
    Abstract: Disclosed is expression of zeta negative and zeta positive nucleic acids or nucleic acid complexes using a dystrophin gene in a process for providing nucleic acid expression in a striated (skeletal or cardiac) muscle cell for the purpose of providing a change to the endogenous properties of the cell for cells affected by muscular dystrophy.
    Type: Application
    Filed: January 10, 2003
    Publication date: July 10, 2003
    Inventors: Sean D. Monahan, Jon A. Wolff, Paul M. Slattum, James E. Hagstrom, Vladimir G. Budker, David B. Rozema
  • Compositions and methods for drug delivery using pH sensitive molecules
    Publication number: 20030026841
    Abstract: An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
    Type: Application
    Filed: March 11, 2002
    Publication date: February 6, 2003
    Inventors: Vladimir S. Trubetskoy, James E. Hagstrom, Vladimir G. Budker, Jon A. Wolff, David B. Rozema, Sean D. Monahan
  • pH-titratable polyampholytes for delivering polyions to a cell
    Publication number: 20030008009
    Abstract: A polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
    Type: Application
    Filed: March 11, 2002
    Publication date: January 9, 2003
    Inventors: Vladimir S. Trubetskoy, James E. Hagstrom, Vladimir G. Budker, Jon A. Wolff, David B. Rozema, Sean D. Monahan
  • Intravascular delivery of non-viral nucleic acid
    Publication number: 20020137707
    Abstract: Disclosed is a system for providing nucleic acid expression in a cell. A polynucleotide is inserted into a mammalian vasculature. The vessel permeability is increased and the polynucleotide is delivered to the cell where it enhances the endogenous properties of the cell.
    Type: Application
    Filed: July 27, 2001
    Publication date: September 26, 2002
    Inventors: Sean D. Monahan, Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, David B. Rozema, Paul M. Slattum
  • Charge reversal of polyion complexes
    Publication number: 20020052335
    Abstract: An ionic polymer is utilized in “recharging” (another layer having a different charge) a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The resulting recharged complex can be formed with an appropriate amount of positive or negative charge such that the resulting complex has the desired net charge.
    Type: Application
    Filed: June 14, 2001
    Publication date: May 2, 2002
    Inventors: David B. Rozema, Sean D. Monahan, Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker
  • Intravascular delivery of non-viral nucleic acid
    Patent number: 6379966
    Abstract: Disclosed is a complex for providing nucleic acid expression in a cell. A polynucleotide and a polymer are mixed together to form the complex wherein the zeta potential of the complex is not positive. Then the complex is delivered to the cell wherein the nucleic acid is expressed.
    Type: Grant
    Filed: November 29, 1999
    Date of Patent: April 30, 2002
    Assignee: Mirus Corporation
    Inventors: Sean D. Monahan, Jon A. Wolff, Paul M. Slattum, James E. Hagstrom, Vladimir G. Budker, David B. Rozema
  • Compositions and methods for drug delivery using amphiphile binding molecules
    Publication number: 20010044412
    Abstract: The present invention relates to the delivery of desired compounds (e.g., nucleic acids) into cells using noncovalent delivery systems which include complexing nucleic acids, amphipathic binding agents, and amphiphiles.
    Type: Application
    Filed: November 29, 2000
    Publication date: November 22, 2001
    Inventors: Jon A. Wolff, James E. Hagstrom, Sean D. Monahan, Vladimir Budker, David B. Rozema, Paul M. Slatum
  • Compound containing a labile disulfide bond
    Publication number: 20010044417
    Abstract: A labile disulfide-containing compound under physiological conditions containing a labile disulfide bond and a transduction signal.
    Type: Application
    Filed: February 8, 2001
    Publication date: November 22, 2001
    Inventors: Jon A. Wolff, Sean D. Monahan, Vladimir G. Budker, Paul M. Slattum, David B. Rozema
  • Compound containing a labile disulfide bond
    Publication number: 20010036926
    Abstract: A labile disulfide-containing compound under physiological conditions, comprising: the disulfide-containing compound having a labile disulfide bond that is either a disulfide bond that is cleaved more rapidly than oxidized glutathione or a disulfide bond constructed from thiols in which one of the constituent thiols has a lower pKa than glutathione or a disulfide bond that is activated by intramolecular attack from a free thiol.
    Type: Application
    Filed: February 28, 2001
    Publication date: November 1, 2001
    Inventors: Jon A. Wolff, Sean D. Monahan, Vladimir G. Budker, Paul M. Slattum, David B. Rozema
  • INTRAVASCULAR DELIVERY OF NON-VIRAL NUCLEIC ACID
    Publication number: 20010019723
    Abstract: Disclosed is a complex for providing nucleic acid expression in a cell. A polynucleotide and a polymer are mixed together to form the complex wherein the zeta potential of the complex is not positive. Then the complex is delivered to the cell wherein the nucleic acid is expressed.
    Type: Application
    Filed: November 29, 1999
    Publication date: September 6, 2001
    Inventors: SEAN D. MONAHAN, JON A. WOLFF, PAUL M. SLATTUM, JAMES E. HAGSTROM, VLADIMIR G. BUDKER, DAVID B. ROZEMA
  • INTRAVASCULAR DELIVERY OF NON-VIRAL NUCLEIC ACID
    Publication number: 20010004636
    Abstract: Disclosed is a process for transfecting genetic material into a mammalian cell to alter endogenous properties of the cell. The process comprises designing a polynucleotide for transfection. Then the polynucleotide is inserted into a mammalian vessel such as a tail vein or artery. Prior to insertion, subsequent to insertion, or concurrent with insertion the permeability of the vessel is increased thereby the genetic material is delivered to the parenchymal cell altering endogenous properties of the cell.
    Type: Application
    Filed: November 23, 1999
    Publication date: June 21, 2001
    Inventors: SEAN D. MONAHAN, JON A. WOLF, PAUL M. SLATTUM, JAMES E. HAGSTROM, VLADIMIR G. BUDKER, DAVID B, ROZEMA
  • Method for refolding misfolded enzymes with detergent and cyclodextrin
    Patent number: 5563057
    Abstract: A method for refolding an enzyme from a misfolded configuration to a second native and active configuration is presented. The method comprises adding a linear alkyl detergent to a misfolded enzyme to form an enzyme-detergent complex. Then the enzyme-detergent complex is contacted with a cyclodextrin to allow the enzyme to assume a second active configuration. The misfolded enzyme may have been previously denatured by heat or by chemical means other than a detergent.
    Type: Grant
    Filed: October 31, 1994
    Date of Patent: October 8, 1996
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Samuel H. Gellman, David B. Rozema