Abstract: A flexible bag having expansible and collapsible cells can be used in a liquid dispenser. A rigid manifold, and in one instance a rigid frame is provided in the bag to keep passages open in use and to isolate one of the cells from the remaining cells. The manifold is capable of altering the volume of one of the cells so that the same bag can be used in different applications.

Abstract: This invention relates to in vitro cell culture employing a fibrin network in a flexible gas permeable container. Specifically, the invention is directed to a cell culture container comprising a flexible, gas permeable material with fibrin matrix which is conducive to the culture of anchorage dependent cells, and the container is suitable for use in closed system in vitro cell culture. The gas permeability of the container is sufficient to permit cellular respiration.

Abstract: An aseptic/sterile fluid connection between two containers is formed by radio frequency sealing together portions of transfer regions of two containers. Each transfer region has a layer of a relatively high melt temperature material such as a rubber or elastomer positioned between two outer container walls made of a flexible thermoplastic, for example polyvinyl chloride. The transfer regions are placed in registry, and the radio frequency welding fuses together portions of adjacent container outer walls and forms a fluid opening therethrough.

Abstract: The present invention provides a large volume flexible container capable of containing a fluid to be maintained under sterile conditions. The container comprises an inner liner constructed from a polyolefin that defines an interior of the container and an outer liner constructed from a three-layer laminate. The laminate includes an inner layer constructed from a polyolefin that will, upon the application of sufficient energy, bond to the polyolefin of the inner liner, a middle layer constructed from a barrier material, and an outer layer; the inner liner and outer liner being sealed together along edges of the container. At least one tube member, for accessing the container, extends from a face of the container, and includes an end that is secured to the container, a length of flexible tubing for defining a channel through which fluid can flow, and a connector at a second end. A structure for housing the container is also provided.

Abstract: A method and apparatus for joining sealingly incompatible plastic materials is disclosed. A coextruded connector (10) with separate, telescopically related layers (12,14) is affixed to one of the materials. The outer layer (14) of connector (10) is composed of a polymer specifically selected to melt or deform at a temperature lower than that of inner layer (12). The connector is frictionally fit within a second material to be joined and the connection is heated to a temperature sufficient to melt or deform outer layer (14) without melting inner layer (12). Outer layer 14 is thereby securely fused to the second material or blood container.A secondary embodiment is disclosed for joining materials of similar composition. Two coextruded connectors (40,46), having telescopically related layers composed of different polymer materials, are formed, as above, but the layer sequence of each connector is opposite to the other.