Patents by Inventor David Brafman

David Brafman has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • TRANSIENT REPORTERS AND METHODS FOR BASE EDITING ENRICHMENT
    Publication number: 20260126430
    Abstract: Provided herein are compositions and methods for real-time identification and isolation of base-edited cell populations. Also provided herein are methods for producing enriched isogenic lines of genetically modified cells, including base-edited human pluripotent stem cells. In particular, provided herein are methods utilizing transient expression of reporter proteins, the detectable signal of which is altered following base editing. Using the transient reporter with a base editor permits enrichment of isogenic populations of base-edited cells.
    Type: Application
    Filed: July 23, 2025
    Publication date: May 7, 2026
    Inventors: David Brafman, Xiao Wang, Nicholas Brookhouser, Stefan Tekel, Kylie Standage-Beier
  • Transient reporters and methods for base editing enrichment
    Patent number: 12405265
    Abstract: Provided herein are compositions and methods for real-time identification and isolation of base-edited cell populations. Also provided herein are methods for producing enriched isogenic lines of genetically modified cells, including base-edited human pluripotent stem cells. In particular, provided herein are methods utilizing transient expression of reporter proteins, the detectable signal of which is altered following base editing. Using the transient reporter with a base editor permits enrichment of isogenic populations of base-edited cells.
    Type: Grant
    Filed: June 14, 2021
    Date of Patent: September 2, 2025
    Assignee: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
    Inventors: David Brafman, Xiao Wang, Nicholas Brookhouser, Stefan Tekel, Kylie Standage-Beier
  • CAS9 Fusion Proteins and Related Methods
    Publication number: 20230193322
    Abstract: Disclosed are recombinant Cas9 proteins, methods of production, and methods of use for targeted DNA deletions, DNA insertions, or both in a eukaryotic genome. An assay system for evaluating the ability of the recombinant Cas9 proteins for targeted DNA deletions, DNA insertions, or both in a eukaryotic genome is also disclosed.
    Type: Application
    Filed: April 14, 2020
    Publication date: June 22, 2023
    Inventors: Kylie Standage-Beier, Parithi Balachandran, Nicholas Brookhouser, David Brafman, Xiao Wang
  • TRANSIENT REPORTERS AND METHODS FOR BASE EDITING ENRICHMENT
    Publication number: 20210389303
    Abstract: Provided herein are compositions and methods for real-time identification and isolation of base-edited cell populations. Also provided herein are methods for producing enriched isogenic lines of genetically modified cells, including base-edited human pluripotent stem cells. In particular, provided herein are methods utilizing transient expression of reporter proteins, the detectable signal of which is altered following base editing. Using the transient reporter with a base editor permits enrichment of isogenic populations of base-edited cells.
    Type: Application
    Filed: June 14, 2021
    Publication date: December 16, 2021
    Inventors: David Brafman, Xiao Wang, Nicholas Brookhouser, Stefan Tekel, Kylie Standage-Beier