Abstract: Areas of interest on three-dimensional maps can be made visually distinct using three-dimensional tint band meshes that overlay other three-dimensional meshes, for example three-dimensional meshes providing terrain, elevation, and infrastructure data. Characteristics of the three-dimensional tint band meshes may be dynamically updated for visual distinctiveness. When overlaying three-dimensional tint band meshes, the overlay operation can use connectivity information of the three-dimensional meshes to increase performance.

Abstract: Compositions and methods for editing a pathogenic ATP-binding cassette, subfamily A, member 4 (ABCA4) polypeptide-encoding gene using an adenosine deaminase base editor to treat a congenital eye disorder, such as Stargardt disease. In various embodiments, the disclosure provides methods for altering a nucleobase (e.g., c.4139T) in an ABCA4 gene codon 1380 encoding a pathogenic leucine so that the codon is altered to encode a proline. In some embodiments, the disclosure provides methods for altering a pathogenic c.5714+5A intronic nucleotide of anABCA4 gene so that the nucleotide becomes c.5714+5G.

Abstract: The invention provides compositions and methods for delivering first and second polynucleotides each encoding a fragment of an A-to-G Base Editor fusion protein comprising one or more deaminases (e.g., adenosine deaminases) and nCas9, wherein the first polynucleotide encodes an N-terminal fragment of nCas9 fused to an intein-N of a split intein pair and the second polynucleotide encodes a C-terminal fragment of nCas9 fused to an intein-C of a split intein pair, and methods for delivering these fragments together with an sgRNA to a cell (e.g., AAV delivery), where the fragments are spliced together by a split intein system, thereby reconstituting a functional base editing system in the cell.

Abstract: This disclosure provides compositions and methods for the editing of a SERPINA1 gene with prime editing.

Abstract: The present disclosure features compositions and methods for editing a 290-KD centrosomal protein (CEP290) gene to treat a congenital eye disorder, such as Leber's Congenital Amaurosis-10. In embodiments, the disclosure provides methods for direct correction of the IVS26 pathogenic mutation in the CEP290 gene (CEP290 c.2991+1655A>G) and/or disruption of a cryptic splice donor site within an intron of the CEP290 gene using a base editor (e.g., a cytidine deaminase base editor, an adenosine deaminase base editor, or a cytidine adenosine deaminase base editor (CABE)).

Abstract: Provided herein are compositions and methods of using base editors comprising a polynucleotide programmable nucleotide binding domain and a nucleobase editing domain in conjunction with a guide polynucleotide. Also provided herein are base editor systems for editing nucleobases of target nucleotide sequences.

Abstract: Provided herein includes high-throughput methods for in vivo screening nanoparticles (e.g., lipid nanoparticles) for preferential delivery to a target tissue or cell type. Also provided are compositions used in the high-throughput nanoparticle screening method.

Abstract: In some embodiments, an electronic device present navigation routes from various perspectives. In some embodiments, an electronic device modifies display of representations of (e.g., physical) objects in the vicinity of a navigation route while presenting navigation directions. In some embodiments, an electronic device modifies display of portions of a navigation route that are occluded by representations of (e.g., physical) objects in a map. In some embodiments, an electronic device presents representations of (e.g., physical) objects in maps. In some embodiments, an electronic device presents representations of (e.g., physical) objects in maps in response to requests to search for (e.g., physical) objects.

Abstract: Provided herein are compositions and methods of using base editors comprising a polynucleotide programmable nucleotide binding domain and a nucleobase editing domain in conjunction with a guide polynucleotide. Also provided herein are base editor systems for editing nucleobases of target nucleotide sequences.

Abstract: The invention features polynucleotides that encode bases editors having a heterologous intron for self-inactivation, compositions comprising such polynucleotides, and methods of inactivating a base editor encoded by such polynucleotides.

Abstract: Areas of interest on three-dimensional maps can be made visually distinct using three-dimensional tint band meshes that overlay other three-dimensional meshes, for example three-dimensional meshes providing terrain, elevation, and infrastructure data. Characteristics of the three-dimensional tint band meshes may be dynamically updated for visual distinctiveness. When overlaying three-dimensional tint band meshes, the overlay operation can use connectivity information of the three-dimensional meshes to increase performance.

Abstract: Provided herein are compositions and methods of using base editors comprising a polynucleotide programmable nucleotide binding domain and a nucleobase editing domain in conjunction with a guide polynucleotide. Also provided herein are base editor systems for editing nucleobases of target nucleotide sequences.

Abstract: Provided herein are compositions and methods of using base editors comprising a polynucleotide programmable nucleotide binding domain and a nucleobase editing domain in conjunction with a guide polynucleotide. Also provided herein are base editor systems for editing nucleobases of target nucleotide sequences.

Abstract: The invention features compositions and methods for altering mutations associated with Rett Syndrome (RTT). Provided herein are compositions and methods of using base editors comprising a polynucleotide programmable nucleotide binding domain and a nucleobase editing domain in conjunction with a guide polynucleotide. Also provided herein are base editor systems for editing nucleobases of target nucleotide sequences.

Abstract: The invention provides compositions and methods for delivering first and second polynucleotides each encoding a fragment of an A-to-G Base Editor fusion protein comprising one or more deaminases (e.g., adenosine deaminases) and nCas9, wherein the first polynucleotide encodes an N-terminal fragment of nCas9 fused to an intein-N of a split intein pair and the second polynucleotide encodes a C-terminal fragment of nCas9 fused to an intein-C of a split intein pair, and methods for delivering these fragments together with an sgRNA to a cell (e.g., AAV delivery), where the fragments are spliced together by a split intein system, thereby reconstituting a functional base editing system in the cell.

Abstract: A system and method for implementing a software emulation environment is provided. In one example, a mobile application can interface with an emulation environment that can be used to test whether the mobile application includes malware that can compromise the security and integrity of an enterprise's computing infrastructure. When the mobile application issues a call for data, a device mimic module can intercept the call and determine if the call includes a call for one or more checkable artifacts that can reveal the existence of the emulation environment. If such a call for data occurs, the device mimic module can provide one or more spoofed checkable artifacts that have been recorded from a real-world mobile device. In this way, the existence of the emulation environment can be concealed so as to allow for a more thorough analysis of a mobile application for potential hidden malware.

Abstract: A system and method for implementing a software emulation environment is provided. In one example, a mobile application can interface with an emulation environment that can be used to test whether the mobile application includes malware that can compromise the security and integrity of an enterprise's computing infrastructure. When the mobile application issues a call for data, a device mimic module can intercept the call and determine if the call includes a call for one or more checkable artifacts that can reveal the existence of the emulation environment. If such a call for data occurs, the device mimic module can provide one or more spoofed checkable artifacts that have been recorded from a real-world mobile device. In this way, the existence of the emulation environment can be concealed so as to allow for a more thorough analysis of a mobile application for potential hidden malware.

Abstract: A system and method for implementing a software emulation environment is provided. In one example, a mobile application can interface with an emulation environment that can be used to test whether the mobile application includes malware that can compromise the security and integrity of an enterprise's computing infrastructure. When the mobile application issues a call for data, a device mimic module can intercept the call and determine if the call includes a call for one or more checkable artifacts that can reveal the existence of the emulation environment. If such a call for data occurs, the device mimic module can provide one or more spoofed checkable artifacts that have been recorded from a real-world mobile device. In this way, the existence of the emulation environment can be concealed so as to allow for a more thorough analysis of a mobile application for potential hidden malware.

Abstract: Systems and methods for managing and implementing secure runtime software hooking on devices are provided. The system and method disclosed includes components and features that enable enterprises and organizations to securely manage mobile devices that have access to the organization's data and network resources. Various embodiments provide for secure systems and methods to modify a behavior of an operating system or one or more applications, without having to flash or modify the Read-only Memory (ROM).

Abstract: A system and method for implementing a software emulation environment is provided. In one example, a mobile application can interface with an emulation environment that can be used to test whether the mobile application includes malware that can compromise the security and integrity of an enterprise's computing infrastructure. When the mobile application issues a call for data, a device mimic module can intercept the call and determine if the call includes a call for one or more checkable artifacts that can reveal the existence of the emulation environment. If such a call for data occurs, the device mimic module can provide one or more spoofed checkable artifacts that have been recorded from a real-world mobile device. In this way, the existence of the emulation environment can be concealed so as to allow for a more thorough analysis of a mobile application for potential hidden malware.