Abstract: A device for detecting nucleic acids in a biological sample has a sample port, a lysis station and a sample conduit configured to mix a sample and lysis agent to form a sample-lysis mixture, pass the sample-lysis mixture across a solid-state membrane to capture nucleic acids in the biological sample therein, and receive the remainder of the sample-lysis mixture in a waste chamber. The wash station is configured to introduce the wash solution following the sample-lysis mixture, pass the wash solution across the solid-state membrane to purify nucleic acids captured therein, and receive the wash solution from the solid-state membrane in the waste chamber. The elution station is configured to pass the eluent across the solid-state membrane, elute captured nucleic acids from the solid-state membrane, and pass the captured nucleic acids into one or more reaction chambers for amplifying and detecting the captured nucleic acids.