Abstract: The present disclosure provides a method for analyzing nucleic acid sequences. The method can comprise determining, by a computer system, a base trace by trimming a Sanger sequencing trace of a plurality of nucleic acid molecules from a sample based on a first target sequence and a second target sequence. Each of the first and second target sequences can be in the plurality of nucleic acid molecules or can be in the complement of sequence of the plurality of nucleic acid molecules.

Abstract: The present disclosure provides a method for designing a set of guide RNAs for hybridizing a genomic region of interest. The present disclosure further provides methods of editing at least one genomic region of interest with at least one set of guide RNAs.

Abstract: The present disclosure provides a method for designing a set of guide RNAs for hybridizing a genomic region of interest. The present disclosure further provides methods of editing at least one genomic region of interest with at least one set of guide RNAs.

Abstract: The present disclosure provides a method for designing a set of guide RNAs for hybridizing a genomic region of interest. The present disclosure further provides methods of editing at least one genomic region of interest with at least one set of guide RNAs.

Abstract: The present disclosure provides a method for designing a set of guide RNAs for hybridizing a genomic region of interest. The present disclosure further provides methods of editing at least one genomic region of interest with at least one set of guide RNAs.

Abstract: The present disclosure provides a method for designing a set of guide RNAs for hybridizing a genomic region of interest. The present disclosure further provides methods of editing at least one genomic region of interest with at least one set of guide RNAs.

Abstract: The present disclosure provides a method for designing a set of guide RNAs for hybridizing a genomic region of interest. The present disclosure further provides methods of editing at least one genomic region of interest with at least one set of guide RNAs.

Abstract: The present disclosure provides a method for analyzing nucleic acid sequences. The method can comprise determining, by a computer system, a base trace by trimming a Sanger sequencing trace of a plurality of nucleic acid molecules from a sample based on a first target sequence and a second target sequence. Each of the first and second target sequences can be in the plurality of nucleic acid molecules or can be in the complement of sequence of the plurality of nucleic acid molecules.

Abstract: The present disclosure provides a method for designing a set of guide RNAs for hybridizing a genomic region of interest. The present disclosure further provides methods of editing at least one genomic region of interest with at least one set of guide RNAs.

Abstract: A preformed liner that expands when filled is provided. In one embodiment, the preformed liner includes a central section having a plurality of flutes that allow the liner to expand. In other embodiments, the preformed liner includes at least a central section that is made of an expandable or stretchable elastomeric material. In still other embodiments, the preformed liner includes a central section having a plurality of flutes and is made of an expandable or stretchable elastomeric material.

Abstract: A gutter cleaning system uses a pump supplied with water from a holding tank to power heads at spaced apart sites along the gutter to flush debris towards down spouting. The power heads are in fluid tight connections with manifold extending along fascia boards. The volume of collected water by the down spouting is returned to the holding tank. The entrained debris is separated by a strainer grate to recover entrained debris from the water flow in the down spout before delivery to the holding tank. A timer provides supervisory control of the operation of the pump.

Abstract: A preformed liner that expands when filled is provided. In one embodiment, the preformed liner includes a central section having a plurality of flutes that allow the liner to expand. In other embodiments, the preformed liner includes at least a central section that is made of an expandable or stretchable elastomeric material. In still other embodiments, the preformed liner includes a central section having a plurality of flutes and is made of an expandable or stretchable elastomeric material.