Abstract: The invention is directed to producing large numbers of cells using hollow fiber bioreactor technology. The cells are non-embryonic stem, non-germ cells that can be characterized by one or more of the following: extended replication in culture and markers of extended replication, such as telomerase, markers of pluripotentiality, and broad differentiation potential, without being transformed.

Abstract: One embodiment of the invention provides culture-expanded canine progenitor cells of non-embryonic origin that can be maintained in culture in the undifferentiated state or differentiated to form cells of multiple tissue types. The culture-expanded cells are post-natal somatic cells that can be characterized by one or more of the following: a population doubling rate of less than about 24 hours; a normal karyotype; can differentiate into at least two cell types of the mesodermal germ layer; extended replication in culture and express markers of extended replication, such as telomerase; and express markers of pluripotency. Also provided are methods of isolation and culture as well as therapeutic uses for the cells, such as treating musculoskeletal and inflammatory diseases and disorders. Additionally provided are cell banks that can be used to provide the cells for administration to a subject, drug discovery methods, and compositions of cells, such as in pharmaceutical compositions.

Abstract: The invention is directed to producing large numbers of cells using hollow fiber bioreactor technology. The cells are non-embryonic stem, non-germ cells that can be characterized by one or more of the following: extended replication in culture and markers of extended replication, such as telomerase, markers of pluripotentiality, and broad differentiation potential, without being transformed.

Abstract: The invention is directed to producing large numbers of cells using hollow fiber bioreactor technology. The cells are non-embryonic stem, non-germ cells that can be characterized by one or more of the following: extended replication in culture and markers of extended replication, such as telomerase, markers of pluripotentiality, and broad differentiation potential, without being transformed.