Abstract: Provided herein is technology relating to nanopore analysis of nucleic acids and particularly, but not exclusively, to compositions, methods, systems, and kits for analysis of nucleic acids or other assays comprising nucleic acid components.

Abstract: Disclosed are methods for multiple displacement amplification of a nucleic acid sequence in a sample. The nucleic acid is contacted with a reaction mixture that includes a set of oligonucleotide primers and a plurality of polymerase enzymes. The reaction mixture is subjected to conditions under which the nucleic acid sequence is amplified to produce an amplification product in a multiple displacement amplification reaction. Also disclosed are kits containing a set of oligonucleotide primers with random sequences having lengths of 6 to 8 nucleobases. At least some of the individual members of the primers have one or more ribose modifications that stabilize or lock the ribose ring in a 3?-endo conformation. At least some of the primers have one or more universal nucleobases.

Abstract: Disclosed are methods for multiple displacement amplification of a nucleic acid sequence in a sample. The nucleic acid is contacted with a reaction mixture that includes a set of oligonucleotide primers and a plurality of polymerase enzymes. The reaction mixture is subjected to conditions under which the nucleic acid sequence is amplified to produce an amplification product in a multiple displacement amplification reaction. Also disclosed are kits containing a set of oligonucleotide primers with random sequences having lengths of 6 to 8 nucleobases. At least some of the individual members of the primers have one or more ribose modifications that stabilize or lock the ribose ring in a 3? -endo conformation. At least some of the primers have one or more universal nucleobases.

Abstract: Content items such as web pages can be updated through a client-driven process when the client detects that a target location reference in a source content item has been redirected to another (destination) location reference. Based on a target location reference in a source content item, a client can request a target content item from a target server and can receive a redirected destination target location reference. The client can update local information based on the redirection and/or report the redirect event to a source server that provided the source content item to the client. The source server can update the source content item based on the client's report.

Abstract: Provided herein are buffers for the stabilization of nucleic acid molecules. The buffers find particular use for the stabilization of trace amounts of nucleic acid molecules in a variety of environments, including repeated freeze/thaw cycles. For example, in some embodiments, provided herein are compositions comprising tris(hydroxymethyl)aminomethane (Tris), ethylenediaminetetraacetic acid (EDTA), polyadenylic acid, and a synthetic DNA oligonucleotide.

Abstract: Provided herein are buffers for the stabilization of nucleic acid molecules. The buffers find particular use for the stabilization of trace amounts of nucleic acid molecules in a variety of environments, including repeated freeze/thaw cycles. For example, in some embodiments, provided herein are compositions comprising tris(hydroxymethyl)aminomethane (Tris), ethylenediaminetetraacetic acid (EDTA), polyadenylic acid, and a synthetic DNA oligonucleotide.

Abstract: Content items such as web pages can be updated through a client-driven process when the client detects that a target location reference in a source content item has been redirected to another (destination) location reference. Based on a target location reference in a source content item, a client can request a target content item from a target server and can receive a redirected destination target location reference. The client can update local information based on the redirection and/or report the redirect event to a source server that provided the source content item to the client. The source server can update the source content item based on the client's report.

Abstract: Provided herein are buffers for the stabilization of nucleic acid molecules. The buffers find particular use for the stabilization of trace amounts of nucleic acid molecules in a variety of environments, including repeated freeze/thaw cycles. For example, in some embodiments, provided herein are compositions comprising tris(hydroxymethyl)aminomethane (Tris), ethylenediaminetetraacetic acid (EDTA), polyadenylic acid, and a synthetic DNA oligonucleotide.

Abstract: The present invention provides oligonucleotide primers, compositions, and kits containing the same for rapid identification of bacteria which are members of the Francisella genus by amplification of a segment of bacterial nucleic acid followed by molecular mass analysis.

Abstract: A kitchen grip or implement for kitchen use is composed of a water-resistant and stain proof material having temperature control characteristics. Such a material forms the implement from at least one sheet having a textured, recessed or irregular surface providing a non-slip contact surface while the opposite surface of the sheet is composed of a close fabricated fabric which is affixed to the sheet of non-slip contact surface material. The fabric sheet may be a sheet of nylon/polyester composition which is affixed to the non-slip contact surface sheet which may be composed of a chloride rubber. The sheets are die cut to the shape of a useful implement such as a hand mitt, a trivet, a lid holder or the like. A two-piece implement such as a hand mitt requires that two sheets of the fabric and chloride rubber composition be sewn together by means of a zigzag lock stitch employing nylon thread.