Abstract: A sample preparation cartridge module comprises an input and output; and interconnected volumes arranged in series between the input and output, the volumes comprising a fluidic isolation chamber and an output channel connected to the fluidic isolation chamber downstream of the fluidic isolation chamber and leading to the output.

Abstract: A seal member includes an annular seal body extending about a flow axis. The seal body has a first sealing face, second sealing face, and a step face. The first sealing face extends radially from the flow axis and has a planar contour. The second sealing face extends radially from the flow axis, is axially opposite the first sealing face, and has an arcuate contour. The step face extends radially from the flow axis and is defined axially between the first sealing face and the second sealing face, the step face arranged for capture between a spout and a reservoir body to capture the seal member. Fluid reservoirs and methods of making fluid reservoirs are also described.