Abstract: A method of capturing a Circulating Tumor Cell (CTC) from a sample includes introducing a sample into a microfluidic device having a cell capture surface and a flow modification surface under conditions that allow a CTC to bind to a cell rolling-inducing agent and a capturing agent disposed on the cell capture surface. The flow modification surface induces a rotational flow within the sample as it flows through the microfluidic device.

Abstract: A method of capturing a Circulating Tumor Cell (CTC) from a sample includes introducing a sample into a microfluidic device having a cell capture surface and a flow modification surface under conditions that allow a CTC to bind to a cell rolling-inducing agent and a capturing agent disposed on the cell capture surface. The flow modification surface induces a rotational flow within the sample as it flows through the microfluidic device.

Abstract: A microfluidic device having a perfusion chamber, the perfusion chamber having a base, a bath opening in the base, a supply inlet and an exhaust outlet. The device further includes a gas permeable membrane attached beneath the perfusion chamber, the gas permeable membrane having a first opening in registration with the supply inlet and a second opening in registration with the exhaust outlet. A substrate is attached to the gas permeable membrane, the substrate having at least one microchannel arranged for flow communication with the supply inlet and the exhaust outlet. In addition, a slide is attached to the substrate. As such, gas introduced through the supply inlet is communicated to the microchannel via the first opening, and the gas permeable membrane is positioned to be exposed to the gas to communicate the gas to the bath opening.

Abstract: An assembly comprises a multiwell test plate having a plurality of wells having an opening and a base surface, an insert plate having a plurality of inserts positioned to align with a corresponding one of the wells whereby the insert plate can be nested with the multiwell plate. Each of the inserts includes a supply port arranged for flow communication with a supply source, an exhaust port, a bottom portion having a plurality of channels extending between the supply port and the exhaust port, and a gas permeable membrane covering the bottom portion. Each of the inserts is sized to position the gas permeable membrane a desired distance from the base surface of the multiwell test plate when the multiwell test plate and the insert plate are coupled to one another.

Abstract: A method of capturing a Circulating Tumor Cell (CTC) from a sample includes introducing a sample into a microfluidic device having a cell capture surface and a flow modification surface under conditions that allow a CTC to bind to a cell rolling-inducing agent and a capturing agent disposed on the cell capture surface. The flow modification surface induces a rotational flow within the sample as it flows through the microfluidic device.

Abstract: An assembly comprises a multiwell test plate having a plurality of wells having an opening and a base surface, an insert plate having a plurality of inserts positioned to align with a corresponding one of the wells whereby the insert plate can be nested with the multiwell plate. Each of the inserts includes a supply port arranged for flow communication with a supply source, an exhaust port, a bottom portion having a plurality of channels extending between the supply port and the exhaust port, and a gas permeable membrane covering the bottom portion. Each of the inserts is sized to position the gas permeable membrane a desired distance from the base surface of the multiwell test plate when the multiwell test plate and the insert plate are coupled to one another.

Abstract: Provided is a microarray platform for the culture of cells atop combinatorial matrix mixtures; enabling the study of differentiation in response to a multitude of microenvironments in parallel.

Abstract: A microparticle storage and delivery device is provided for allowing an individual to selectively dispense a dosage of a microparticle. The device includes a hydrogel string loaded with a plurality of dosages of the microparticle and a storage container for receiving the string. The storage container has a first open end and a second opposite end. A biasing structure urges the string toward the open end of the storage container and an actuator selectively engages the string to urge a dosage of the microparticle from the open end of the storage container.

Abstract: A microfluidic device is provided for delivering a drug to an individual. The microfluidic device includes a body that defines a reservoir for receiving the drug therein. A valve interconnects the reservoir to an output needle that is insertable into the skin of an individual. A pressure source urges the drug from the reservoir toward the needle. The valve is movable between a closed position preventing the flow of the drug from the reservoir to the output needle and an open position allowing for the flow of the drug from the reservoir to the output needle in response to a predetermined condition in the physiological fluids of the individual.