Abstract: Systems and methods for laser capture microdissection are disclosed. An inverted microscope includes a translation stage joystick subsystem. The systems and methods provide the advantages of increased speed and much lower rates of contamination.

Abstract: Systems and methods for laser capture microdissection are disclosed. A method of laser capture microdissection includes providing a sample that is to undergo laser capture microdissection; positioning said sample within an optical axis of a laser capture microdissection instrument; providing a transfer film carrier having a substrate surface and a laser capture microdissection transfer film coupled to said substrate surface; placing said laser capture microdissection transfer film in juxtaposition with said sample with a pressure sufficient to allow laser capture microdissection transfer of a portion of said sample to said laser capture microdissection transfer film, without forcing nonspecific transfer of a remainder of said sample to said laser capture microdisection film; and then transferring a portion of said sample to said laser capture microdissection transfer film, without forcing nonspecific transfer of a remainder of said sample to said laser capture microdissection transfer film.

Abstract: Systems and methods are described for barriers on laser capture microdissection samples. A method of processing a biological sample for laser capture microdissection, includes: providing the biological sample; and applying a substance to the biological sample so as to provide a barrier between the biological sample and a surrounding environment. The systems and methods provide advantages because non-specific pick-up is reduced, visualization is improved, sample degradation is reduced, and contamination is reduced.

Abstract: The present invention generally discloses an extraction system that provides a locale for fluid processing and extraction on a post-microcapture transfer film. The extraction system includes a transfer film carrier and an extraction device forming a reservoir. The extraction system selectively excludes regions of the transfer film from the reservoir to advantageously reduce contamination due to matter adhered to the transfer film by non-specific transfer.

Abstract: Systems and methods for laser capture microdissection are disclosed. An inverted microscope includes a vacuum chuck subsystem that is adapted to hold a microscope slide down during the process of laser capture microdissection. The systems and methods provide the advantages of increased speed and much lower rates of contamination.

Abstract: Systems and methods for acquiring laser capture microdissection samples are disclosed. An integral portion of a biological reaction vessel includes a transfer film carrier having a substrate surface; and a laser capture microdissection transfer film coupled to the substrate surface of the transfer film carrier. The systems and methods facilitate quick and accurate laser capture microdissection while simultaneously minimizing contamination.

Abstract: The present invention generally provides an improved transfer film having multiple layers for laser micro-capture of a sample. The transfer film for laser micro-capture includes distinct layers for expansion and adhesion in order to optimize the performance of the transfer film. A transfer film including a spring-back layer is also disclosed.

Abstract: A thermoplastic film for LCM tissue transfer is thermally coupled to a broadband energy-absorbing material. The broadband energy-absorbing material may either be introduced into the film composition as a dopant or may be in thermal contact with the film.

Abstract: Systems and methods for laser capture microdissection are disclosed. A method of laser capture microdissection includes providing a sample that is to undergo laser capture microdissection; positioning said sample within an optical axis of a laser capture microdissection instrument; providing a transfer film carrier having a substrate surface and a laser capture microdissection transfer film coupled to said substrate surface; placing said laser capture microdissection transfer film in juxtaposition with said sample with a pressure sufficient to allow laser capture microdissection transfer of a portion of said sample to said laser capture microdissection transfer film, without forcing nonspecific transfer of a remainder of said sample to said laser capture microdisection film; and then transferring a portion of said sample to said laser capture microdissection transfer film, without forcing nonspecific transfer of a remainder of said sample to said laser capture microdissection transfer film.

Abstract: Systems and methods for laser capture microdissection are disclosed. An inverted microscope includes an illumination/laser beam delivery system that is adapted to both illuminate a sample and provide energy for laser capture microdissection of the sample. The systems and methods provide the advantages of increased speed and much lower rates of contamination.

Abstract: Systems and methods for laser capture microdissection are disclosed.

Abstract: A laser capture microdissection lift-off substrate comprising a transparent thermoplastic film that is incorporated into substrate which may take the form of a cap or other structure associated with an analysis vessel or which may be used with a holder or other element of a LCM tissue sample transfer apparatus. The thermoplastic lift-off substrate film is held in a fixed position on the substrate which may be relative to a feature of a biological analysis vessel. The film may be affixed to a substrate which is a structure which goes into the vessel such that the sample is held in a fixed position relative to an observation port on the vessel.

Abstract: Systems and methods for acquiring laser capture microdissection samples are disclosed. A method of making a laser capture microdissection consumable includes providing a transfer film carrier having a substrate surface; and fabricating a laser capture microdissection transfer film on said substrate surface, wherein forming includes hot vacuum baking said laser capture microdissection transfer film. The systems and methods facilitate quick and accurate laser capture microdissection while simultaneously minimizing contamination.

Abstract: A laser capture microdissection lift-off substrate comprising a transparent thermoplastic film is incorporated into substrate which may take the form of a cap or other structure associated with an analysis vessel or which may be used with a holder or other element of a LCM tissue sample transfer apparatus. The thermoplastic lift-off substrate film is held in a fixed position on the substrate which may be relative to a feature of a biological analysis vessel. The film may be affixed to a substrate which is a structure which goes into the vessel such that the sample is held in a fixed position relative to an observation port on the vessel.

Abstract: A laser system includes a laser diode source with a plurality of output facets. The laser diode source produces a divergent output along an emission path. A microlens is optically coupled to the diode source. The microlens receives the divergent output along the emission path and modifies the divergence of the output in a high numerical aperture direction of the microlens A power source is coupled to the laser diode source.

Abstract: A small diameter multimode optical fiber with a low numerical aperture (i.e., 0.1) is used as a microlens to collimate the output emissions of a laser diode before butt coupling the output of the laser diode to an optical fiber. The optical fiber used as the microlens is chosen such that its diameter roughly equals the diameter of the fiber to be coupled to the laser diode. One end of the fiber is mounted in a fixed position and the other end of the fiber is slip fit into the end of a hollow receiving member mounted in a fixed position and running parallel to the axis of the fiber. The collimation is performed in the high NA direction of the output of the laser diode. The output of a bundle of butt coupled optical fibers may be used to pump a laser system.

Abstract: A Fourier Transform Raman Spectrometer system includes a folded V laser cavity. A first leg of the folded V laser cavity is defined by a highly reflective end mirror and a dichroic fold mirror and a second leg of the folded V laser cavity is defined by the fold mirror and an output coupler. A solid-state laser gain medium is disposed in the first leg of the folded V laser cavity and is pumped by a pump source aligned with the optical path of the first leg of the laser cavity and radiating through the fold mirror. The fold mirror is highly transmissive at the wavelength of pump radiation from the pump source and highly reflective at the laser output wavelength. An output beam from the laser is passed through a tuneable filter comprising an acousto-optic device and is directed at a sample to be analyzed. Light reflected from the sample is directed to a Raman Spectrometer for analysis.

Abstract: In a laser amplifier a high efficiency pumping scheme mode matches the TEMOO laser mode volume with a plurality of linearly spaced laser diode pumping sources positioned along a lateral side of a block of laser material. The optical path within the block is configured in a tightly folded zig-zag configuration. Pump radiation from the diode pumping sources is collimated by an optical fiber end the fold angle is selected to mode match the pump radiation to the mode volume.A laser oscillator is followed by one or more cascaded amplifier stages including a block of laser material arranged in a tightly folded zig-zag configuration. Pump radiation from the diode pumping sources is collimated by an optical fiber and the fold angle is selected to mode match the pump radiation to the mode volume.

Abstract: A dispersion compensation delay line is added to a synchronously pumped fiber Raman ring oscillator to generate subpicosecond pulses. A pair of spaced gratings form the delay line. An interference filter is used as a bandwidth limiting tuning element to provide a good quality short pulse. An integrated design eliminates the discrete optical elements by using only an optical fiber and an optical fiber coupler.

Abstract: A window assembly for a laser uses a uniaxial crystalline material such as quartz for the window, secured directly in a hard seal to a metal tubular end component. In order to avoid significant differential thermal expansion between the quartz window and the end component, the quartz crystal is cut in a Z-normal configuration, whereby the thermal expansion and other physical properties of the window are isotropic in the plane of the window. The ability to make a reliable direct seal to a metal component offsets any disadvantages of the Z-normal configuration thought to be controlling in the prior art. Through the disclosed method for assembling and mounting the window, reflection loses are reduced to a practical minimum comparable to that of any preferred conventional window orientation.