Abstract: Apparatus and methods are disclosed for viewing low-birefringence structures within samples directly, with the eye, in real-time. The sample is placed between an entrance polarizer and analyzer polarizer, the transmission state of one of which is changed dynamically to create a modulated view of the scene; against this background, birefringent structures are visible because of their different appearance when modulated. Modulation rates of 4 or more states per second; use of 4 or more states, or even a continuum of states, which lie substantially on a latitude line on the Poincare sphere; and orientation of the polarization components to produce a uniform background; produce a clear view that does not produce operator fatigue. Broad-band wavelength operation spanning 50 nm or more, or the whole visible range, is achieved, and it is compatible with integration into other microscopy modes such as Hoffman relief contrast.

Abstract: Apparatus and methods are disclosed for viewing low-birefringence structures within samples directly, with the eye, in real-time. The sample is placed between an entrance polarizer and analyzer polarizer, the transmission state of one of which is changed dynamically to create a modulated view of the scene; against this background, birefringent structures are visible because of their different appearance when modulated. Modulation rates of 4 or more states per second; use of 4 or more states, or even a continuum of states, which lie substantially on a latitude line on the Poincare sphere; and orientation of the polarization components to produce a uniform background; produce a clear view that does not produce operator fatigue. Broad-band wavelength operation spanning 50 nm or more, or the whole visible range, is achieved, and it is compatible with integration into other microscopy modes such as Hoffman relief contrast.

Abstract: The present invention relates to an imaging apparatus and comprises input and output polarisers, a first polarising beam splitter and at least one additional polarising beam splitter, a light sensitive detector and focussing means arranged on an axis. The input polariser resolves incident light into a single linear polarisation state. The first polarising beam splitter receives light from the input polarises, and resolves it into equal magnitude orthogonally polarised rays which are mutually spaced and have a path difference therebetween. The or each additional polarising beam splitter is arranged to receive light from the first polarising beam splitter. The transmission axis of the output polariser is parallel to or perpendicular to the transmission axis of the input polarises to resolve the orthogonally polarised light rays having past through the or each additional polarising beam splitter into the same or perpendicular polarisation state as light resolved by the, first polariser.

Abstract: An imaging spectrometer is disclosed that comprises imaging means for dividing a received image into two or more spatially separated images and means for detecting each spectral image, and is characterised in that the imaging means comprises at least one polarizing beam splitter. The polarizing beam splitter may be a Wollaston prism. In one embodiment of the invention, the imaging means comprises image replication means to produce two or more spatially separated images, and one or more filter elements such as dichroic filters which act to alter the spectral characteristics of one or more of the spatially separated images. In a further embodiment of the invention the imaging means comprises one or more spectral replication means arranged in optical series, each spectral replication means comprising an optical retardation element and a polarising beam splitter.