Patents by Inventor David Harrow Gelfand

David Harrow Gelfand has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • DNA polymerases and related methods
    Patent number: 10035993
    Abstract: Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
    Type: Grant
    Filed: July 6, 2017
    Date of Patent: July 31, 2018
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Keith A. Bauer, Ellen Fiss, David Harrow Gelfand, Edward S. Smith, Shawn Suko, Thomas W. Myers
  • DNA POLYMERASES AND RELATED METHODS
    Publication number: 20170369856
    Abstract: Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
    Type: Application
    Filed: July 6, 2017
    Publication date: December 28, 2017
    Inventors: Keith A. BAUER, Ellen FISS, David Harrow GELFAND, Edward S. SMITH, Shawn SUKO, Thomas W. MYERS
  • DNA polymerases and related methods
    Patent number: 9738876
    Abstract: Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
    Type: Grant
    Filed: January 20, 2015
    Date of Patent: August 22, 2017
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Keith A. Bauer, Ellen Fiss, David Harrow Gelfand, Edward S. Smith, Shawn Suko, Thomas W. Myers
  • DNA POLYMERASES AND RELATED METHODS
    Publication number: 20160024548
    Abstract: Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. The mutant polymerases overcome the inhibitory effects of a variety of polymerase and reverse transcriptase inhibitors. Therefore, the mutant polymerases are useful in a variety of disclosed methods in the presence of such inhibitors.
    Type: Application
    Filed: July 27, 2015
    Publication date: January 28, 2016
    Inventors: Keith A. Bauer, Ellen Fiss, David Harrow Gelfand, Edward S. Smith, Shawn Suko, Thomas W. Myers, Joseph San Filippo, Rachel Shahinian
  • DNA POLYMERASES AND RELATED METHODS
    Publication number: 20150218537
    Abstract: Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
    Type: Application
    Filed: January 20, 2015
    Publication date: August 6, 2015
    Inventors: Keith A. Bauer, Ellen Fiss, David Harrow Gelfand, Edward S. Smith, Shawn Suko, Thomas W. Myers
  • 2?-terminator nucleotide-related methods and systems
    Patent number: 8163487
    Abstract: The present invention provides methods of extending primer nucleic acids and sequencing target nucleic acids. The methods include the use of 2?-terminator nucleotides to effect chain termination. In addition to related reaction mixtures and kits, the invention also provides computers and computer readable media.
    Type: Grant
    Filed: March 10, 2011
    Date of Patent: April 24, 2012
    Assignee: Roche Molecular Systems, Inc.
    Inventors: David Harrow Gelfand, Fred Lawrence Reichert, Veeraiah Bodepudi, Amar Gupta, Stephen Will, Thomas W. Myers
  • Mutant DNA polymerases with improved pyrophosphorolysis activated polymerization (PAP) ability
    Patent number: 8071536
    Abstract: Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
    Type: Grant
    Filed: November 26, 2008
    Date of Patent: December 6, 2011
    Assignee: Roche Molecular System, Inc.
    Inventors: Keith A Bauer, David Harrow Gelfand
  • DNA polymerases and related methods
    Patent number: 8026091
    Abstract: Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
    Type: Grant
    Filed: July 9, 2008
    Date of Patent: September 27, 2011
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Keith A. Bauer, David Harrow Gelfand
  • 2'-Terminator Nucleotide-Related Methods and Systems
    Publication number: 20110201002
    Abstract: The present invention provides methods of extending primer nucleic acids and sequencing target nucleic acids. The methods include the use of 2?-terminator nucleotides to effect chain termination. In addition to related reaction mixtures and kits, the invention also provides computers and computer readable media.
    Type: Application
    Filed: March 10, 2011
    Publication date: August 18, 2011
    Applicant: ROCHE MOLECULAR SYSTEMS, INC.
    Inventors: David Harrow Gelfand, Fred Lawrence Reichert, Veeraiah Bodepudi, Amar Gupta, Stephen Will, Thomas Myers
  • Synthesis and compositions of 2'-terminator nucleotides
    Patent number: 7947817
    Abstract: The invention provides compositions that comprise nucleotides and/or nucleosides having blocking groups at 2?-positions of sugar moieties. Methods of synthesizing these nucleic acids are also provided.
    Type: Grant
    Filed: June 28, 2004
    Date of Patent: May 24, 2011
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Veeraiah Bodepudi, Stephen Gordon Will, David Harrow Gelfand
  • 2?-Terminator nucleotide-related methods and systems
    Patent number: 7919249
    Abstract: The present invention provides methods of extending primer nucleic acids and sequencing target nucleic acids. The methods include the use of 2?-terminator nucleotides to effect chain termination. In addition to related reaction mixtures and kits, the invention also provides computers and computer readable media.
    Type: Grant
    Filed: July 16, 2008
    Date of Patent: April 5, 2011
    Assignee: Roche Molecular Systems, Inc.
    Inventors: David Harrow Gelfand, Fred Lawrence Reichert, Veeraiah Bodepudi, Amar Gupta, Stephen Will, Thomas Myers
  • Thermostable DNA polymerases incorporating nucleoside triphosphates labeled with fluorescent dyes
    Patent number: 7598032
    Abstract: Modified thermostable DNA polymerases having enhanced efficiency for incorporating unconventional nucleotides such as those labeled with fluorescein family dyes are advantageous in many in vitro DNA synthesis applications. Such enzymes are particularly useful for use in chain termination nucleic acid sequencing protocols, as are native forms of such enzymes. Genes encoding the modified enzymes and methods for their production and use offer cost and efficiency advantages for DNA sequencing.
    Type: Grant
    Filed: January 30, 2003
    Date of Patent: October 6, 2009
    Assignee: Roche Molecular Systems
    Inventors: David Harrow Gelfand, Lisa Vivian Kalman, Fred Lawrence Reichert, Christopher Lim Sigua, Thomas W. Myers
  • 2?-terminator nucleotide-related methods and systems
    Patent number: 7572581
    Abstract: The present invention provides methods of extending primer nucleic acids and sequencing target nucleic acids. The methods include the use of 2?-terminator nucleotides to effect chain termination. In addition to related reaction mixtures and kits, the invention also provides computers and computer readable media.
    Type: Grant
    Filed: June 28, 2004
    Date of Patent: August 11, 2009
    Assignee: Roche Molecular Systems, Inc.
    Inventors: David Harrow Gelfand, Fred Lawrence Reichert, Veeraiah Bodepudi, Amar Gupta, Stephen Will, Thomas Myers
  • Mutant DNA Polymerases with Improved Pyrophasphorolysis Activated Polymerization (PAP) Ability
    Publication number: 20090155802
    Abstract: Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
    Type: Application
    Filed: November 26, 2008
    Publication date: June 18, 2009
    Applicant: ROCHE MOLECULAR SYSTEMS, INC.
    Inventors: Keith A. Bauer, David Harrow Gelfand
  • 2'-Terminator Nucleotide-Related Methods and Systems
    Publication number: 20090142810
    Abstract: The present invention provides methods of extending primer nucleic acids and sequencing target nucleic acids. The methods include the use of 2?-terminator nucleotides to effect chain termination. In addition to related reaction mixtures and kits, the invention also provides computers and computer readable media.
    Type: Application
    Filed: July 16, 2008
    Publication date: June 4, 2009
    Applicant: ROCHE MOLECULAR SYSTEMS, INC.
    Inventors: David Harrow Gelfand, Fred Lawrence Reichert, Veeraiah Bodepudi, Amar Gupta, Stephen Will, Thomas W. Myers
  • DNA POLYMERASES AND RELATED METHODS
    Publication number: 20090137000
    Abstract: Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
    Type: Application
    Filed: July 9, 2008
    Publication date: May 28, 2009
    Applicant: Roche Molecular Systems, Inc.
    Inventors: Keith A. Bauer, David Harrow Gelfand
  • High temperature reverse transcription using mutant DNA polymerases
    Patent number: 7179590
    Abstract: The present invention relates to improved reverse transcription methods using a modified thermostable DNA polymerases, particularly in a magnesium ion buffer. These methods are particularly useful in combined reverse-transcription/amplification reactions.
    Type: Grant
    Filed: March 30, 2001
    Date of Patent: February 20, 2007
    Assignee: Roche Molecular Systems, Inc
    Inventors: Edward Soh Smith, Carita Maria Elfstrom, David Harrow Gelfand, Russell Gene Higuchi, Thomas William Myers, Nancy Jeneane Schönbrunner, Alice Ming Wang
  • Thermostable DNA polymerases incorporating nucleoside triphosphates labeled with fluorescent dyes
    Publication number: 20030152988
    Abstract: Modified thermostable DNA polymerases having enhanced efficiency for incorporating unconventional nucleotides such as those labeled with fluorescein family dyes are advantageous in many in vitro DNA synthesis applications. Such enzymes are particularly useful for use in chain termination nucleic acid sequencing protocols, as are native forms of such enzymes. Genes encoding the modified enzymes and methods for their production and use offer cost and efficiency advantages for DNA sequencing.
    Type: Application
    Filed: January 30, 2003
    Publication date: August 14, 2003
    Inventors: David Harrow Gelfand, Lisa Vivian Kalman, Fred Lawrence Reichert, Christopher Lim Sigua, Thomas W. Myers
  • Thermostable DNA polymerases incorporating nucleoside triphosphates labeled with fluorescein family dyes
    Publication number: 20020142333
    Abstract: Modified thermostable DNA polymerases having enhanced efficiency for incorporating unconventional nucleotides such as those labeled with fluorescein family dyes are advantageous in many in vitro DNA synthesis applications. Such enzymes are particularly useful for use in chain termination nucleic acid sequencing protocols, as are native forms of such enzymes. Genes encoding the modified enzymes and methods for their production and use offer cost and efficiency advantages for DNA sequencing.
    Type: Application
    Filed: January 17, 2002
    Publication date: October 3, 2002
    Applicant: F. Hoffmann-La Roche AG.
    Inventors: David Harrow Gelfand, Lisa Vivian Kalman, Fred Lawrence Reichert, Christopher Lim Sigua, Thomas W. Myers
  • Thermostable DNA polymerases incorporating nucleoside triphosphates labeled with fluorescein family dyes
    Patent number: 6346379
    Abstract: Modified thermostable DNA polymerases having enhanced efficiency for incorporating unconventional nucleotides such as those labeled with fluorescein family dyes are advantageous in many in vitro DNA synthesis applications. Such enzymes are particularly useful for use in chain termination nucleic acid sequencing protocols, as are native forms of such enzymes. Genes encoding the modified enzymes and methods for their production and use offer cost and efficiency advantages for DNA sequencing.
    Type: Grant
    Filed: September 3, 1998
    Date of Patent: February 12, 2002
    Assignee: F. Hoffman-La Roche AG
    Inventors: David Harrow Gelfand, Lisa Vivian Kalman, Fred Lawrence Reichert, Christopher Lim Sigua, Thomas W. Myers