Abstract: Prescription profile information for corrective lenses used in conjunction with a near-eye display device is obtained by the near-eye display device to facilitate correction of aberrations introduced by the corrective lenses. The prescription profile information can be transmitted electronically to the near-eye display device, embedded on the lens surface of the corrective lens itself using a near invisible marking such as near-infrared ink that can be detected by a gaze-tracking camera of the near-eye display device, or the near-eye display device can infer the prescription profile information for the corrective lenses based on the locations of glints reflected by the corrective lenses and detected by the gaze-tracking camera. Based on the prescription profile information, the near-eye display device calibrates the display, sensors, and/or gaze-tracking circuitry to correct aberrations introduced by the corrective lenses.

Abstract: An imaging system includes an optical mounting plate having an aperture disposed between a first portion and a second portion, an imaging sensor disposed on the first portion, an objective lens disposed on the second portion, and an illumination assembly arranged within the aperture and disposed between the imaging sensor and the objective lens. The imaging system includes a heat sink coupled to the illumination assembly and configured to transfer heat away from light sources of the illumination assembly via a working fluid that flows through the heat sink.

Abstract: An imaging system, comprising: a tube lens; a first image sensor; a second image sensor; an objective disposed to direct emission light from a focal plane of the objective to the tube lens. The first image sensor and the second image sensor are arranged at focal planes of the tube lens. A beamsplitter is disposed along a first optical axis between the tube lens and the first image sensor to intercept the path of the emission light.

Abstract: Provided herein are methods of detecting a target nucleic acid of interest for spatial gene expression analysis in a sample using barcoded templated ligation probes.

Abstract: Provided herein are methods for minimizing Z-image acquisition comprising receiving a first set of three-dimensional (3D) positional information of a plurality of biological molecules within a sample, wherein the first set of 3D positional information is within a first imaging volume and based on a probing cycle of the sample in an imaging instrument, wherein the probing cycle comprises generating optical signals corresponding to at least some of the plurality of biological molecules; determining, based on the first set of 3D positional information, a second imaging volume that is less than the first imaging volume; and directing the imaging instrument to image the second imaging volume in at least one subsequent probing cycle of the sample.

Abstract: In an x-ray source, an isolation circuit can isolate bias voltage at a cathode from a bias voltage at an alternating current source (AC source). The isolation circuit can transfer alternating current from the AC source to the cathode. The isolation circuit can be made repeatedly with minimal variation or failed parts, can be light, and can be small. The isolation circuit can include planar transformer(s). Each planar transformer can include a primary trace on a primary circuit board and a secondary trace on a secondary circuit board. The primary trace and the secondary trace can each include a spiral shape. The primary trace can be located in close proximity to the secondary trace such that alternating electrical current through the primary trace will induce alternating electrical current through the secondary trace.

Abstract: The subject disclosure encompasses solutions for matching customer information with customer reviews. A process of the disclosed technology includes steps for aggregating customer data associated with a customer, retrieving a plurality of customer reviews, wherein each of the customer reviews describe customer satisfaction with a service provided by a corresponding service provider, extracting review metadata for each of the plurality of customer reviews, and matching the customer with at least one of the customer reviews based on the customer data and the extracted review metadata. Systems and machine-readable media are also provided.

Abstract: Disclosed are assemblies, systems, and methods for transilluminating a sample disposed within a device. An assembly includes a first optically transparent substrate configured to receive a biological sample, a second optically transparent substrate having a top surface, a bottom surface, and a plurality of sides. The first substrate contacts the top surface of the second substrate. The assembly includes at least one light source configured to illuminate at least one of the plurality of sides of the second substrate and a light scattering layer on the bottom surface of the second substrate. The light scattering layer is configured to scatter light from the light source. The assembly includes a thermal control module coupled to the second substrate and configured to control the temperature of the second substrate.

Abstract: Methods and systems for performing multi-resolution in situ decoding are described. The method may comprise, for example, acquiring at least one first image of a biological sample at a first optical resolution; identifying locations for a plurality of target analytes based on the at least one first image; acquiring at least one second image of the biological sample at a second optical resolution in at least one decoding cycle of a plurality of decoding cycles used for in situ decoding of the target analytes; and extracting signal intensity data for signals associated with all or a portion of the target analytes from the at least one second image based on the locations for the target analytes identified in the at least one first image. In some instances, the method may further comprise using the signal intensity data extracted from the at least one second image to decode the target analytes.

Abstract: Techniques for spectral unmixing and decoding for in situ analysis are provided. Input hypercube data comprising voxel data, raw channel data, and decoding round data are obtained, and an initial iteration of codeword data determination is performed. Performing the initial iteration includes generating initial feature data and initial unmixed fluorescence data, generating initial uncorrected codeword data, and generating initial corrected codeword data. One or more subsequent iterations of codeword data determination are then performed based on the input hypercube data and based on feature data, unmixed fluorescence data, uncorrected codeword data, and corrected codeword data from one or more previous iterations. When it is determined that one or more convergence conditions have been satisfied, output comprising an optimized estimate of fluorescence data, and an optimized estimate of uncorrected codeword data, and an optimized estimate of corrected codeword data is generated.

Abstract: An imaging system includes an optical mounting plate having an aperture disposed between a first portion and a second portion, an imaging sensor disposed on the first portion, an objective lens disposed on the second portion, and an illumination assembly arranged within the aperture and disposed between the imaging sensor and the objective lens. The imaging system includes a heat sink coupled to the illumination assembly and configured to transfer heat away from light sources of the illumination assembly via a working fluid that flows through the heat sink.

Abstract: An imaging system includes an optical mounting plate having an aperture disposed between a first portion and a second portion, an imaging sensor disposed on the first portion, an objective lens disposed on the second portion, and an illumination assembly arranged within the aperture and disposed between the imaging sensor and the objective lens. The imaging system includes a heat sink coupled to the illumination assembly and configured to transfer heat away from light sources of the illumination assembly via a working fluid that flows through the heat sink.

Abstract: Various embodiments of the present disclosure disclose mitigating internal and external vibrational disturbances in opto-fluidic instrument. In various embodiments, the resonance frequency ranges and/or the vibrational frequency ranges of various modules and components of the opto-fluidic instrument may be selected to avoid resonances from occurring in the opto-fluidic instrument, thereby mitigating vibrations from internal and external sources that may distort the imaging of samples in the opto-fluidic instrument.

Abstract: The present disclosure is directed to a power tool including a motor housing and an electric motor situated within the motor housing. The electric motor is configured to provide rotational energy to rotate an output device of the power tool about an output axis. The power tool also includes a cover configured to cover at least a portion of a surface of the motor housing that faces the output device. The power tool further includes a first lighting device located within the cover and a second lighting device located within the cover. The first lighting device and the second lighting device are located around the output axis and are separated from each other by at least 130 degrees about the output axis. The power tool also includes first power wires configured to provide power to the first lighting device from a power source of the power tool.

Abstract: A tilted structured illumination pattern for use in an optical system is generated, wherein the tilted structured illumination pattern is not symmetrical about a center point of a back pupil of the system, and wherein the tilted structured illumination pattern is based on exactly two wave vectors and defines spatial frequencies along an optical axis of an objective of the system. The tilted structured illumination pattern is directed to be incident on a sample, such that one or more fluorophores of the sample are excited by the structured illumination pattern. A phase of the tilted structured illumination pattern is varied. A set of images of fluorescence emission emitted by the sample is captured, wherein each image of the set of images corresponds to a respective phase of the tilted structured illumination pattern and to a respective axial imaging plane. Combined image data is generated based on the set of images.

Abstract: In an x-ray source, an isolation circuit can isolate bias voltage at a cathode from a bias voltage at an alternating current source (AC source). The isolation circuit can transfer alternating current from the AC source to the cathode. The isolation circuit can be made repeatedly with minimal variation or failed parts, can be light, and can be small. The isolation circuit can include planar transformer(s). Each planar transformer can include a primary trace on a primary circuit board and a secondary trace on a secondary circuit board. The primary trace and the secondary trace can each include a spiral shape. The primary trace can be located in close proximity to the secondary trace such that alternating electrical current through the primary trace will induce alternating electrical current through the secondary trace.

Abstract: The present disclosure relates to methods for preparing biological samples for in situ analysis of one or more analytes wherein the biological sample has been previously affixed to a substrate, which in some cases may not be compatible with in situ analysis, for example, due to the absence of positional markers and/or fiducial markers and/or a region suitable for in situ signal detection on the substrate. In some aspects, a hydrophobic adapter having positional markers and/or fiducial markers is applied to the substrate to which a biological sample has been affixed, thus enabling in situ sample processing and analysis of the biological sample. The hydrophobic adhesive label or adapter can be used for automated microscope alignment and sample preparation.

Abstract: It would be advantageous to reduce weight and size of high voltage power supplies, to increase frequency of pulses of high voltage, and to improve control of magnitude of high voltage. The embodiments of high voltage power supplies described herein can solve these problems. The high voltage power supply can be used with an x-ray tube. The high voltage power supply can comprise an array of planar transformers each defining a stage with an AC input and a DC output. Each stage can comprise a pair of flat, coil windings adjacent one another and including a primary winding electrically-coupled to the AC input and configured to induce a current in a secondary winding. At least two stages can be electrically-coupled together in series with the DC output of one stage electrically-coupled to an input of the other stage such that a voltage is amplified across the stages.

Abstract: The subject disclosure encompasses solutions for matching customer information with customer reviews. A process of the disclosed technology includes steps for aggregating customer data associated with a customer, retrieving a plurality of customer reviews, wherein each of the customer reviews describe customer satisfaction with a service provided by a corresponding service provider, extracting review metadata for each of the plurality of customer reviews, and matching the customer with at least one of the customer reviews based on the customer data and the extracted review metadata. Systems and machine-readable media are also provided.

Abstract: It would be advantageous to reduce weight and size of high voltage power supplies, to increase frequency of pulses of high voltage, and to improve control of magnitude of high voltage. The embodiments of high voltage power supplies described herein can solve these problems. The high voltage power supply can be used with an x-ray tube. The high voltage power supply can comprise an array of planar transformers each defining a stage with an AC input and a DC output. Each stage can comprise a pair of flat, coil windings adjacent one another and including a primary winding electrically-coupled to the AC input and configured to induce a current in a secondary winding. At least two stages can be electrically-coupled together in series with the DC output of one stage electrically-coupled to an input of the other stage such that a voltage is amplified across the stages.