Abstract: The present invention describes the identification, isolation, sequencing and characterization of two human CalDAG-GEF, and two human cAMP-GEF genes, which are associated with the Ras pathway. Also identified are CalDAG-GEF gene homologues in mice and cAMP-GEF gene homologues in rats. Nucleic acids and proteins comprising or derived from the CalDAG-GEFs and/or cAMP-GEFs are useful in screening and diagnosing certain Ras-associated cancers, in identifying and developing therapeutics for treatment of certain Ras-associated cancers, and in producing cell lines and transgenic animals useful as models of Ras-associated cancers.

Abstract: The present invention is based, in part, on assays we conducted that revealed compounds that may be used to treat or prevent diseases characterized by an abnormal or undesirable association of one protein with another.

Abstract: The present invention is based, in part, on the discovery of methods for identifying compounds that mediate (by promoting or inhibiting) protein-protein interaction (e.g., aggregation, dimerization, or other physiologially significant association). Compounds that mediate such interaction, which are also within the scope of the invention, can be used to treat Alzheimer's disease, disorders associated with expanded CAG repeats (such as Huntington's disease), and disorders in which polyglutamine-containing transcription factors or coactivators are undesirably active (e.g., disorders associated with homodimerization ofjun or hexamerization of p53.

Abstract: Methods for determining the presence of polymorphisms in estrogen receptor genes and assessing an individual's risk for developing a condition are provided herein.

Abstract: A method is described for multiplexed detection of polymorphic sites and direct determination of haplotypes in DNA fragments, DNA, and genomic DNA, using single-walled carbon nanotube (SWNT) atomic force microscopy (AFM) probes. This technique has applications for haplotyping in population-based genetic disease studies and other genomic screening.

Abstract: A process of isolating and identifying cloned DNA segments from defined regions of a chromosome by preparing a series of cell hybrids from a first species and a second species, which hybrids contain a chromosome or deletion mutant of a chromosome as the oThe Government has rights in this invention pursuant to Contract Number CA-5-14051 awarded by the Department of Health and Human Services, Contract Number PCM 77-17747 awarded by the National Science Foundation, and Contract Number 1-713 awarded by the National Foundation-March of Dimes.