Patents by Inventor David J. Ledden

David J. Ledden has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10371643
    Abstract: A chemiluminescent detection system, kits and microfluidics devices containing same, and methods of use thereof, are disclosed.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: August 6, 2019
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventor: David J. Ledden
  • Patent number: 10371661
    Abstract: Chemiluminescent detection systems, kits, and microfluidics devices containing same, as well as methods of production and use thereof, are disclosed.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: August 6, 2019
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventor: David J. Ledden
  • Publication number: 20190170759
    Abstract: Chemiluminescent detection systems, kits, and microfluidics devices containing same, as well as methods of production and use thereof, are disclosed. A capture antibody is attached to a surface of a microfluidics device for capturing a sandwich complex formed of target analyte bound by sensitizer and a composition comprising a single oxygen-activatable chemiluminescent compound.
    Type: Application
    Filed: February 6, 2019
    Publication date: June 6, 2019
    Applicant: Siemens Healthcare Diagnostics Inc.
    Inventors: David J. Ledden, Roland Janzen
  • Patent number: 10288628
    Abstract: Kits, microfluidics devices, and assays for use in methods of spectroscopically determining a ratio of glycated hemoglobin to total hemoglobin in a whole blood sample are disclosed.
    Type: Grant
    Filed: April 10, 2015
    Date of Patent: May 14, 2019
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventor: David J. Ledden
  • Patent number: 10105701
    Abstract: A microfluidic distributing device having a plurality of microchannels for the analysis of a fluid sample (such as blood). The microfluidic distributing device has a fluid sample entry port from which subsamples of the fluid sample are distributed to the plurality of microchannels in which fluid subsamples are treated for analysis by test devices.
    Type: Grant
    Filed: July 11, 2016
    Date of Patent: October 23, 2018
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventors: David J. Ledden, Dafeng Chen, James E. Rasmussen
  • Publication number: 20170176419
    Abstract: A chemiluminescent detection system, as well as kits and microfluidics devices containing same, are disclosed. Methods of using the system, kits, and devices are also disclosed. The first, second, and third antibodies or binding fragments thereof may be provided in any form that allows these antibodies/binding fragments to function in accordance with the presently disclosed and claimed inventive concept(s). For example, each of the first, second, and third antibodies/binding fragments may be a polyclonal antibody/binding fragment or a monoclonal antibody/binding fragment.
    Type: Application
    Filed: March 24, 2015
    Publication date: June 22, 2017
    Applicant: Siemens Healthcare Diagnostics Inc.
    Inventors: Jay J. LI, David J. LEDDEN, Eric Scott COWDEN, Donglai LU
  • Publication number: 20170113217
    Abstract: Inventive concepts disclosed herein are directed to a capillary tube and a force assisted sample ejection mechanism. The capillary tube may be at least one of dissolvable or breakable. The force assisted sample ejecting mechanism releasing at least part of the sample of the first liquid from the capillary tube by at least one of abruptly shaking the capillary tube at least once or breaking the capillary tube.
    Type: Application
    Filed: June 11, 2015
    Publication date: April 27, 2017
    Applicant: Siemens Healthcare Diagnostics Inc.
    Inventors: David J. Ledden, Janine A. Cox
  • Publication number: 20170108516
    Abstract: In one aspect, the inventive concepts disclosed herein are directed to a chromatographic assay device for detecting the presence of free hemoglobin in a whole blood sample. The device comprising a chromatographic detection pad with a sample application site and a detection side. The chromatographic detection pad defines a path for capillary fluid flow. The chromatographic detection pad has a pore size. The sample application site on the chromatographic detection pad is for application of a portion of the whole blood sample. The detection site on the chromatographic detection pad is spaced apart from the application site and is downstream of the sample application site. The chromatographic detection pad is devoid of a compound located downstream of the application site that is reactive to the whole blood sample.
    Type: Application
    Filed: June 8, 2015
    Publication date: April 20, 2017
    Applicant: Siemens Healthcare Diagnostics Inc.
    Inventors: David J. Ledden, Janine A. Cox, Jeffrey R. Jasperse
  • Publication number: 20170030932
    Abstract: Kits, microfluidics devices, and assays for use in methods of spectroscopically determining a ratio of glycated hemoglobin to total hemoglobin in a whole blood sample are disclosed.
    Type: Application
    Filed: April 10, 2015
    Publication date: February 2, 2017
    Applicant: Siemens Healthcare Diagnostics Inc.
    Inventor: David J. Ledden
  • Publication number: 20160318019
    Abstract: A microfluidic distributing device having a plurality of microchannels for the analysis of a fluid sample (such as blood). The microfluidic distributing device has a fluid sample entry port from which subsamples of the fluid sample are distributed to the plurality of microchannels in which fluid subsamples are treated for analysis by test devices.
    Type: Application
    Filed: July 11, 2016
    Publication date: November 3, 2016
    Applicant: Siemens Healthcare Diagnostics Inc.
    Inventors: David J. Ledden, Dafeng Chen, James E. Rasmussen
  • Patent number: 9416776
    Abstract: A microfluidic distributing device having a plurality of microchannels for the analysis of a fluid sample (such as blood). The microfluidic distributing device has a fluid sample entry port from which subsamples of the fluid sample are distributed to the plurality of microchannels in which fluid subsamples are treated for analysis by test devices.
    Type: Grant
    Filed: March 13, 2014
    Date of Patent: August 16, 2016
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventors: David J. Ledden, Dafeng Chen, James E. Rasmussen
  • Publication number: 20160033443
    Abstract: Chemiluminescent detection systems, kits, and microfluidics devices containing same, as well as methods of production and use thereof, are disclosed.
    Type: Application
    Filed: March 14, 2014
    Publication date: February 4, 2016
    Applicant: Siemens Healthcare Diagnostics Inc.
    Inventor: David J. Ledden
  • Publication number: 20160033417
    Abstract: A chemiluminescent detection system, kits and microfluidics devices containing same, and methods of use thereof, are disclosed.
    Type: Application
    Filed: March 14, 2014
    Publication date: February 4, 2016
    Applicant: Siemens Healthcare Diagnostics Inc.
    Inventor: David J. Ledden
  • Publication number: 20160025736
    Abstract: Chemiluminescent detection systems, kits and microfluidics devices containing same, as well as methods of production and use thereof, are disclosed. Immunoassay technologies are widely used in the field of medical diagnostics. One example of a commercially used immunoassay is the induced luminescence Immunoassay (LOCI) technology. The currently available LOCI?? technology involves a homogeneous assay {i.e., no wash steps involved) that has high sensitivity, and the assay uses several reagents and requires that two of these reagents (referred to as a ““sensibead” and a “chemibead”) held by other immunoassay reagents to be in dose proximity to achieve a signal.
    Type: Application
    Filed: March 13, 2014
    Publication date: January 28, 2016
    Applicant: Siemens Healthcare Diagnostics Inc.
    Inventors: David J. LEDDEN, Roland JANZEN
  • Publication number: 20160008813
    Abstract: A microfluidic distributing device having a plurality of microchannels for the analysis of a fluid sample (such as blood). The microfluidic distributing device has a fluid sample entry port from which subsamples of the fluid sample are distributed to the plurality of microchannels in which fluid subsamples are treated for analysis by test devices.
    Type: Application
    Filed: March 13, 2014
    Publication date: January 14, 2016
    Applicant: Siemens Healthcare Diagnostic Inc.
    Inventors: David J. LEDDEN, Dafeng CHEN, James E. RASMUSSEN
  • Patent number: 8101429
    Abstract: This invention is directed to a lateral flow assay for detecting the presence of an analyte in a liquid test sample. The lateral flow assay represents an improvement in the ability to accurately and with high fidelity to detect the presence or absence of a target analyte in a liquid sample, in part, by encompassing a reference region of immobilized, non-diffusible analyte that allows for detection of any factors that interfere with the interaction and binding of the analyte to the labeled capture reagent. Any influences on the interaction and binding of the analyte that is free in solution in the liquid test sample to its complementary labeled reagent will be encountered in parallel in the binding between the immobilized analyte in reference region to the labeled reagent as it diffuses through the reference region. In one embodiment, the lateral flow assay of the invention is a urine-based human Chorionic Gonadotropin (hCG) assay.
    Type: Grant
    Filed: June 3, 2004
    Date of Patent: January 24, 2012
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventors: Gary H. Krauth, David J. Ledden
  • Patent number: 7538336
    Abstract: Identifying a test product having a test region includes impinging on the test region a set of test signals at known test wavelengths. An image of the test region is generated from the reflected signals. The image is comprised of a set of reflectance values that are compared against reference reflectance values at the test signal wavelengths to determine the test product type.
    Type: Grant
    Filed: June 3, 2004
    Date of Patent: May 26, 2009
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventors: David A. Brock, Chris T. Zimmerle, David J. Ledden
  • Patent number: 7499154
    Abstract: A readhead for illuminating a sample carrier and receiving light from the sample carrier, including a housing for receiving a sample carrier, an array of light sources mounted within the housing in a fixed position relative to the sample carrier, and including first and second light-emitting diodes for emitting substantially monochromatic light of a two different wavelengths, a light guide mounted in the housing between the light-emitting diodes and the sample carrier, and a light detector coupled to receive light from the sample carrier. The readhead also includes a light source for directing excitation light of a predetermined wavelength to the sample carrier, and a light filter positioned between the sample carrier and the light detector and adapted to prevent passage therethrough of the excitation light. The readhead allows both fluorescence spectroscopy and reflectance spectroscopy to be conducted on the sample carrier.
    Type: Grant
    Filed: June 3, 2004
    Date of Patent: March 3, 2009
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventors: Nicholas T. Stock, Gary D. Ross, David J. Ledden
  • Patent number: 6093546
    Abstract: The invention relates to a process for making a test element, such as an analytical test element. Compositions of matter, such as analyte specific receptors, are treated to carry a charge. These are then applied to test carriers treated to carry a charge opposite that of the composition of matter. Interaction of charge fixes the composition of matter to the test element.
    Type: Grant
    Filed: September 3, 1992
    Date of Patent: July 25, 2000
    Assignee: Roche Diagnostics
    Inventors: David J. Ledden, David R. Moorman, Michael J. Kinch, Harvey B. Buck
  • Patent number: 5492813
    Abstract: Muteins of enzyme acceptor polypeptide fragments of .beta.-galactosidase are provided which exhibit substantially increased kinetic complementation activity with no significant loss in stability. A preferred enzyme acceptor fragment has an amino acid other than cysteine located at position 500 of the natural sequence. An especially preferred substitution is serine or valine. Other preferred muteins have an amino acid other than methionine located at position 443, with leucine being especially preferred, or an amino acid other than cysteine at position 76, with leucine being an especially preferred substitution. Also provided are methods for producing the novel muteins, reagent compositions comprising the novel muteins, and immunoassay methods for determining an analyte in which the novel mutein recombines with an enzyme donor polypeptide fragment to form enzymatically active .beta.-galactosidase.
    Type: Grant
    Filed: November 1, 1993
    Date of Patent: February 20, 1996
    Assignee: Boehringer Mannheim Corporation
    Inventors: Scott J. Eisenbeis, Sophie J. Boguslawski, Mark Krevolin, David J. Ledden