Abstract: Methods are provided herein for assembling at least two nucleic acids using a sequence specific nuclease agent (e.g., a gRNA-Cas complex) to create end sequences having complementarity and subsequently assembling the overlapping complementary sequences. The nuclease agent (e.g., a gRNA-Cas complex) can create double strand breaks in dsDNA in order to create overlapping end sequences or can create nicks on each strand to produce complementary overhanging end sequences. Assembly using the method described herein can assemble any nucleic acids having overlapping sequences or can use a joiner oligo to assemble sequences without complementary ends.

Abstract: A method of calibrating a device comprising: imaging an object in a viewfinder of a device; obtaining a device location from a location mechanism and a device orientation from an orientation mechanism; and using the obtained device location and device orientation to calibrate one or more of the location mechanism and the orientation mechanism such that a difference between an expected location of the object in the viewfinder and a location of the object in the viewfinder is reduced.

Abstract: An oven door includes an interior panel and an exterior panel. The interior panel faces the heating chamber and has a glass sheet for viewing the heating chamber. The exterior panel is affixed to the interior panel and includes a frame portion formed of plastic defining an aperture aligned with the glass sheet for viewing the heating chamber. A non-opaque panel is fixed relative to the frame portion and extends across the aperture. The non-opaque panel has an inside surface facing the interior panel and the exterior panel has a masking portion formed of plastic and integrally formed with the frame portion. The masking portion extends from the frame portion into the aperture along the inside surface of the non-opaque panel and defines a window for viewing the heating chamber through the glass sheet.

Abstract: A method of calibrating a device comprising: imaging an object in a viewfinder of a device; obtaining a device location from a location mechanism and a device orientation from an orientation mechanism; and using the obtained device location and device orientation to calibrate one or more of the location mechanism and the orientation mechanism such that a difference between an expected location of the object in the viewfinder and a location of the object in the viewfinder is reduced.

Abstract: Content items may be transmitted and synchronized between a receiving device and a content hosting service (e.g., a file sharing service) using a coordinating device. The coordinating device may be in and/or connected to a local network of the local device and may facilitate communications between the local device and the remote service. Alternatively, the coordinating device may be remotely connected to the local device. The coordinating device may monitor for new content information on either the local device or the remote service (or both) and propagate new or updated content as appropriate. The coordinating device may further perform functions such as reformatting content, maintaining a database of content identifiers and synchronizing metadata associated with content items.

Abstract: Systems and methods applicable, for example, in distance functionality. Distances might, for instance, be ascertained. One or more heights, one or more optical values, information corresponding to orientation, and/or one or more values corresponding to captured images might, for example, be employed. Ascertained distances might, for example, be updated. An area captured by a device of a first user might, for instance, be recognized as captured by a device of a second user. Such recognition might, for example involve the use of one or more distances between the two users.

Abstract: A bag holder including a main enclosure body for practically enclosing a space shaped and configured to hold bags by their handles, the space being essentially surrounded by the main enclosure body, the main enclosure body having two ends proximate to each other which are separated by a gap and a retractable clip member connected to a first one of the main enclosure body ends and being capable to extend to and engage the second end such that engagement of the clip with the second end completes the enclosure formed by the main enclosure body, wherein the clip member is selectively positioned by the user as to open or close the gap. A method of using the bag is also disclosed.

Abstract: The present invention relates to graphic or informational articles that may be applied to a surface of a translucent or substantially clear substrate so that the image may be viewed through the substrate. The graphic articles of the present invention include a perforated imageable component and a non-perforated attachment component. The imageable component includes an opaque, perforated imageable film layer that accepts an image layer on its first major surface. On the second major surface of the imageable film layer, a perforated, opaque light absorbing film is applied.

Abstract: Sheeting material is disclosed that contains a retroreflective layer and a layer of luminescent material on the bottom or lower surface of the retroreflective layer. The sheeting material can be formed into signage or decorative articles that are highly visible under a variety of lighting conditions. A portion of the retroreflective layer is visible through the luminescent layer or through one or more openings in the luminescent layer which are in the form of slits, perforations, alphanumeric or other patterns. The luminescent layer can be imaged using analog or digital printing processes. The sheeting material can be made from materials that are suitable for back-lit illumination. Adhesives or other conventional attachment means can be employed to anchor the signs in interior or exterior environments.

Abstract: The present invention provides adhesive film constructions that include a detectable transparent release liner. The release liner includes a transparent polymeric core and one or more detection stripes. The detection stripes are parallel to the longitudinal length of the construction. By providing a release liner that is transparent over a majority of its major surfaces (except for the detection stripes), the problem of edge detection when using transparent liners and/or transparent adhesive film constructions can be addressed without significantly degrading the ability to see through a majority of the film and liner composite.

Abstract: The present invention relates to graphic or informational articles that may be applied to a surface of a translucent or substantially clear substrate so that the image may be viewed through the substrate. The graphic articles of the present invention include a perforated imageable component and a non-perforated attachment component. The imageable component includes an opaque, perforated imageable film layer that accepts an image layer on its first major surface. On the second major surface of the imageable film layer, a perforated, opaque light absorbing film is applied. The attachment component includes a substantially transparent scrim layer of a polymeric film. The scrim layer is un-perforated and includes a pressure sensitive adhesive on its first major surface a heat activated adhesive on its second major surface. After the imageable film layer is imaged, the heat activated adhesive may be used to laminate the attachment component to the imageable component to create a finished graphic article.

Abstract: A stirring pitcher which is easy to break down for cleaning and which has a uniquely shaped container that has at its bottom a trough with a contour that matches the contour of the stirrer spoon end. The stirrer includes a handle portion that extends above a container cover, and a stirrer portion which extends below the cover. Preferably the stirrer portion is detachable from the handle portion. The stirrer includes a pivot which is transverse to the longitudinal direction of the container trough, so that pivoting of the spoon about the pivot causes the spoon end to sweep the complete volume adjacent the container bottom.

Abstract: A stirring pitcher which is easy to break down for cleaning and which has a uniquely shaped container that has at its bottom a trough with a contour that matches the contour of the stirrer spoon end. The stirrer includes a handle portion that extends above a container cover, and a stirrer portion which extends below the cover. Preferably the stirrer portion is detachable from the handle portion. The stirrer includes a pivot which is transverse to the longitudinal direction of the container trough, so that pivoting of the spoon about the pivot causes the spoon end to sweep the complete volume adjacent the container bottom.

Abstract: A device that holds a pitcher at a preselected angle of tilt while the pitcher is being filled with a beverage. Where beer is the beverage, the angle of tilt ensures that the beer will flow down the inner side walls of the pitcher, thereby suppressing the foam or head that would develop if the pitcher were charged while in an upright disposition. The weight of the beer as it gradually fills the pitcher gradually overcomes the bias that causes the initial tilting so that the head is continually suppressed throughout the charging operation and so that the pitcher is completely upright when filled. The device saves its user from having to hold a heavy pitcher at a tilted position throughout the charging process.

Abstract: A method is described of producing a laminated structural material which includes the steps of stacking alternately layers of thermoplastics sheet material and one or more layers of material to be laminated, the material having been impregnated with a second thermoplastics material prior to stacking, placing the stack in a pre-heated mould or press and subjecting the stack to heat and pressure for a time sufficient to cause the thermoplastics material to flow and bond together the layers of structural material to be laminated and allowing the mould or press to cool sufficiently to avoid distortion of the moulded part before removal from the mould or press.

Abstract: A material suitable for thermosetting includes a collection of hollow particles adhesively mixed with a thermosetting resin in such proportions that the mixture exists in a granular form and may be converted by thermosetting into a fused solid mass having a density not greater than 0.5 grams per cubic centimeter.The particles may be glass microbubbles or phenolic microballoons and the resin may be a hardenable Friedel-Crafts resin. The material according to the invention is more suitable for packing into shaped cavities than known resins including those containing filler particles.