Patents by Inventor David J. Sharkey

David J. Sharkey has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same
    Patent number: 5622822
    Abstract: Nucleic acids can be made available for amplification or other treatment after lysis by contacting the lysate with polyethyleneimine to form a precipitate with the nucleic acids. The nucleic acids are then released from the precipitate by contact with a strong base, and the released nucleic acids are kept in solution with an anionic phosphate ester surfactant. This method for preparing specimen samples is simple and quite rapid.
    Type: Grant
    Filed: September 13, 1994
    Date of Patent: April 22, 1997
    Assignee: Johnson & Johnson Clinical Diagnostics, Inc.
    Inventors: Tobias E. Ekeze, John W. Backus, David J. Sharkey, Richard C. Sutton, JoAnne H. Kerschner
  • Thermostable polymerase specific antibody-containing DNA amplification composition and kit
    Patent number: 5587287
    Abstract: Antibodies which are specific to a thermostable DNA polymerase can be used to reduce or eliminate the formation of non-specific products in polymerase chain reaction methods. These antibodies and other temperature sensitive inhibitors are effective to inhibit DNA polymerase enzymatic activity at a certain temperature T.sub.1 which is generally below about 85.degree. C. The inhibitors are irreversibly inactivated at temperature T.sub.2 which is generally above about 40.degree. C. T.sub.2 is also greater than T.sub.1. Such inhibitors can be supplied individually or in admixture with the DNA polymerase in a diagnostic test kit suitable for PCR.
    Type: Grant
    Filed: April 7, 1994
    Date of Patent: December 24, 1996
    Assignee: Johnson & Johnson Clinical Diagnostics, Inc.
    Inventors: Edward R. Scalice, David J. Sharkey, Kenneth G. Christy, Jr., Theodore W. Esders, John L. Daiss
  • DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
    Patent number: 5338671
    Abstract: Antibodies which are specific to a thermostable DNA polymerase can be used to reduce or eliminate the formation of non-specific products in polymerase chain reaction methods. These antibodies and other temperature sensitive inhibitors are effective to inhibit DNA polymerase enzymatic activity at a certain temperature T.sub.1 which is generally below about 85.degree. C. The inhibitors are irreversibly inactivated at temperature T.sub.2 which is generally above about 40.degree. C. T.sub.2 is also greater than T.sub.1. Such inhibitors can be supplied individually or in admixture with the DNA polymerase in a diagnostic test kit suitable for PCR.
    Type: Grant
    Filed: October 7, 1992
    Date of Patent: August 16, 1994
    Assignee: Eastman Kodak Company
    Inventors: Edward R. Scalice, David J. Sharkey, Kenneth G. Christy, Jr., Theodore W. Esders, John L. Daiss