Abstract: The invention relates to a method for modifying a template double stranded polynucleotide, especially for characterization using nanopore sequencing. The method produces from the template a plurality of modified double stranded polynucleotides. These modified polynucleotides can then be characterized.

Abstract: Methods of characterizing an analyte using a nanopore. One aspect features methods for characterizing a double-stranded polynucleotide using a nanopore, e.g., without using a hairpin connecting a template and a complement of the double-stranded polynucleotide. Another aspect features methods for characterizing an analyte using a tag-modified nanopore with increased sensitivity and/or higher throughput. Compositions and systems including, e.g., adaptors for attachment to double-stranded polynucleotides and tag-modified nanopores, which can be used in the methods are also provided.

Abstract: An apparatus includes a housing coupled to a medicament container, which is coupled to a needle. An injection assembly is disposed within the housing and includes an energy storage member and an actuation rod. A distal end portion of the actuation rod is disposed within the medicament container. The energy storage member can produce a force on a proximal end portion of the actuation rod sufficient to move the distal end portion of the actuation rod within the medicament container. This can convey at least a portion of a substance from the medicament container via the needle when a distal tip of the needle is disposed within a first region of a target location. The force is insufficient to move the distal end portion of the actuation rod within the medicament container when the distal tip of the needle is disposed within a second region of the target location.

Abstract: A portable vehicle alignment system has a vertical central column with a carriage movable along its length, and a pair of camera arms pivotably attached to the carriage, each with a camera pod. The camera pods each have a camera for capturing image data of a respective vehicle-mounted target. One pod also has a calibration target disposed in a known relationship to its camera, and the other pod has a calibration camera disposed in a known relationship to its camera for capturing images of the calibration target. The camera arms pivot between an extended position where the cameras are disposed to capture image data of the vehicle targets and the calibration camera is disposed to capture images of the calibration target, and a folded position where the aligner has a width smaller than the width between the camera pods.

Abstract: Disclosed are apparatus and related methods for adjusting the pitch of a string on a stringed instrument. In one embodiment, the disclosed devices enable a musician to adjust the tautness and, accordingly, the pitch of individual strings of a stringed instrument by simply pushing a lever or rotating a threaded piston or on or adjacent to the bridge of the guitar. The disclosed devices can preferably be outfitted on electric or acoustic stringed instruments, including guitars. The more specific aspects of the preferred embodiments are discussed in greater detail with reference to the figures.

Abstract: A vehicle wheel alignment system has a plurality of cameras, each camera for viewing a respective target disposed at a respective wheel of the vehicle and capturing image data of the target as the wheel and target are continuously rotated a number of degrees of rotation without a pause. The image data is used to calculate a minimum number of poses of the target of at least one pose for every five degrees of rotation as the wheel and target are continuously rotated the number of degrees of rotation without a pause. At least one of the cameras comprises a data processor for performing the steps of preprocessing the image data, and calculating an alignment parameter for the vehicle based on the preprocessed image data.

Abstract: A wheel alignment system includes a side-to-side reference including an active reference pod and a passive reference pod disposed on opposite sides of the vehicle. The active reference pod includes a reference image sensor fixedly attached to a reference target, for mounting on a first side of the vehicle such that the reference image sensor produces image data including a perspective representation of the passive reference pod disposed on a second/opposite side of the vehicle. In operation, alignment cameras on the opposite sides of the vehicle capture perspective representations of targets mounted to vehicle wheels and of targets of the active and passive reference pods. A computer processes the image data to compute an alignment measurement of the vehicle based on a spatial relationship between the active reference pod and the passive reference pod determined according to the image data produced by the reference image sensor.

Abstract: A wheel alignment system includes a side-to-side reference including an active reference pod and a passive reference pod disposed on opposite sides of the vehicle. The active reference pod includes a reference image sensor fixedly attached to a reference target, for mounting on a first side of the vehicle such that the reference image sensor produces image data including a perspective representation of the passive reference pod disposed on a second/opposite side of the vehicle. In operation, alignment cameras on the opposite sides of the vehicle capture perspective representations of targets mounted to vehicle wheels and of targets of the active and passive reference pods. A computer processes the image data to compute an alignment measurement of the vehicle based on a spatial relationship between the active reference pod and the passive reference pod determined according to the image data produced by the reference image sensor.

Abstract: A telephone call processor for processing telephone calls comprising voice signals and data signals, the call processor comprising a first telephone interface and a second telephone interface, the call processor being operable in a first mode and in a second mode. In the first mode, the call processor is adapted to receive voice signals and data signals at the first telephone interface and to transmit voice signals and data signals via the second telephone interface. In the second mode, the call processor is adapted to receive voice signals and data signals at the first telephone interface, to block data signals from being transmitted via the second telephone interface and optionally to transmit voice signals via the second telephone interface.

Abstract: A process is presented where a feed stream containing a hydrogen sulfide and another feed component is introduced into an absorber that the feed stream flows upward from the bottom of the absorber and contacts a liquid treatment solution, where the liquid treatment solution contains a sulfur dye catalyst. The hydrogen sulfide is absorbed into the liquid treatment solution and converted into sulfide ions. The other feed component is removed from the absorber vessel substantially free of the hydrogen sulfide and a spent treatment solution is also removed from the absorber vessel and fed to an oxidation vessel where it is contacted with an oxygen containing gas causing the sulfide ions to oxidize to thiosulfate and converting the spent sulfur dye catalyst to regenerated sulfur dye catalyst. The thiosulfate is recovered, and the regenerated sulfur dye catalyst can be recycled as part of the liquid treatment solution.

Abstract: A method of fabricating an object, comprising depositing a first layer of first object material on a support substrate electrode; applying a conductive agent material onto the first layer; depositing a first layer of charged powder on an intermediate substrate; conveying the first layer of charged powder on the intermediate substrate proximate to the first layer of first object material; and applying a transferring electric field to cause transfer of the first layer of charged powder from the intermediate substrate onto the first layer on the support substrate electrode, to form a first powder layer on the first layer on the support substrate electrode. Multiple powder layers may be deposited on the first layer. The method may be further comprised of fusing the powder layer(s) to form a first fused layer on the support substrate electrode. A related object fabrication apparatus is also disclosed.

Abstract: A method of playing a side game concurrently playable with a game of roulette includes accepting side bet wagers from players in a first side game during a wager phase for the game of roulette. The side bet wagers are independent of wagers placed on the roulette game. Two or more side game symbols are selected from a first symbol set which are playable in the game of roulette. Each of the side game symbols are assigned a payout value, such that at least one of the assigned payout values is a fixed payout value utilized for each play of the side game and another of the assigned payout values is a variable payout value. A determination is made if any of the selected side game symbols match a winning roulette symbol. Side game prizes are awarded to winning side bet players according to the corresponding assigned payout value.

Abstract: A vehicle wheel alignment system has a plurality of cameras, each camera for viewing a respective target disposed at a respective wheel of the vehicle and capturing image data of the target as the wheel and target are continuously rotated a number of degrees of rotation without a pause. The image data is used to calculate a minimum number of poses of the target of at least one pose for every five degrees of rotation as the wheel and target are continuously rotated the number of degrees of rotation without a pause. At least one of the cameras comprises a data processor for performing the steps of preprocessing the image data, and calculating an alignment parameter for the vehicle based on the preprocessed image data.

Abstract: The present disclosure relates to aerosol delivery devices. The aerosol delivery devices include mechanisms configured to deliver an aerosol precursor composition from a reservoir to an atomizer including a heating element to produce a vapor. A pressure controller may control dispensing of the aerosol precursor composition from the reservoir based on a pressure differential between a first pressure within the reservoir and a second pressure proximate the atomizer. The first pressure within the reservoir may be substantially equal to atmospheric pressure. Alternatively, the first pressure within the reservoir may be greater than atmospheric pressure.

Abstract: An apparatus includes a housing coupled to a medicament container, which is coupled to a needle. An injection assembly is disposed within the housing and includes an energy storage member and an actuation rod. A distal end portion of the actuation rod is disposed within the medicament container. The energy storage member can produce a force on a proximal end portion of the actuation rod sufficient to move the distal end portion of the actuation rod within the medicament container. This can convey at least a portion of a substance from the medicament container via the needle when a distal tip of the needle is disposed within a first region of a target location. The force is insufficient to move the distal end portion of the actuation rod within the medicament container when the distal tip of the needle is disposed within a second region of the target location.

Abstract: A portable vehicle alignment system is provided having two base tower assemblies, each having a pedestal, a columnar tower removably attachable to the top of the pedestal, and a camera pod movable along a length of the tower; and a data processor with a wireless communication device for processing image data from the camera pods. Each camera pod includes a camera for capturing image data of a target mounted on a vehicle, and a communication device for wirelessly communicating with the data processor. One pod has a calibration target and the other pod has a calibration camera for capturing images of the calibration target. The pedestals each have a manually-operated clamp for removably fixedly attaching the tower to the pedestal in one of a plurality of positions such that the orientation of the camera pod to the pedestal is angularly adjustable, allowing horizontal rotation of the camera pod.

Abstract: A wheel alignment system includes a side-to-side reference including an active reference pod and a passive reference pod disposed on opposite sides of the vehicle. The active reference pod includes a reference image sensor fixedly attached to a reference target, for mounting on a first side of the vehicle such that the reference image sensor produces image data including a perspective representation of the passive reference pod disposed on a second/opposite side of the vehicle. In operation, alignment cameras on the opposite sides of the vehicle capture perspective representations of targets mounted to vehicle wheels and of targets of the active and passive reference pods. A computer processes the image data to compute an alignment measurement of the vehicle based on a spatial relationship between the active reference pod and the passive reference pod determined according to the image data produced by the reference image sensor.

Abstract: The invention relates to a method for modifying a template double stranded polynucleotide, especially for characterisation using nanopore sequencing. The method produces from the template a plurality of modified double stranded polynucleotides. These modified polynucleotides can then be characterised.

Abstract: Some embodiments of the present invention generally relate to devices and methods for determining and/or isolating cells. For example, one aspect is generally directed to methods and devices for detecting, identifying, counting, and/or potentially sorting cells of interest in blood or other biological sample. In some embodiments, blood samples (or other biological fluids) may be treated with signaling entities, such as pH-sensitive entities, that change color or otherwise produce a signal in suitable internal environments. For example, certain cells, such as cancer or fetal cells, may have differences in intracellular pH compared to other cells, which can be detected using pH-sensitive entities. In certain embodiments, the cells may be sorted based on such signaling entities; for example, illumination of cells in a suitable machine for sorting cells (e.g., using fluorescent light) may allow determination of the cells, which may also be recovered or isolated for further manipulation in some cases.