Abstract: Systems and methods for providing an advertisement marketplace where buyers and sellers can trade linear mediacast advertisement inventory programmatically at local, national, and/or worldwide levels. The marketplace system may include a seller-controlled marketplace system which connects sellers with buyers and offers revenue management tools for optimizing yields across direct and programmatic channels. The advertisement inventory is bought and sold as canonical inventory units which group similar but discrete advertisement slots together. The marketplace system may include a real-time bidding (RTB) adapter through which digital advertising buyers may place bids for such inventory units using standard protocols utilized in digital RTB systems.

Abstract: The invention relates to a method of preparing and using a library of template polynucleotides suitable for use as templates in solid-phase nucleic acid amplification and sequencing reactions to determine the methylation status of the cytosine bases in the library. In particular, the invention relates to a method of preparing and analysing a library of template polynucleotides suitable for methylation analysis.

Abstract: Systems and methods for dynamically maintaining and utilizing walking profiles for time estimations in service scheduling are provided. A networked system detects usage of an application on a user device. In response to the detecting, the networked system accesses environmental condition data at a location associated with a user of the user device, whereby the environmental condition data comprises one or more environmental condition affecting the location. The networked system accesses walking pace data from the user device, whereby the walking pace data represents a current walking pace of the user and correlates the walking pace data with the environmental condition data. Using the correlated data, the networked system adjusts a parameter in a walking profile of the user. The walking profile is then used to determine time estimates that are used to schedule services that require walking by a user.

Abstract: An interactive visualization system is disclosed herein. The system includes a data source, user input device, processor, and display. The data source obtains a B cell receptor and/or T cell receptor data source. The user input device receives a user selected parameter under which to analyze the data set. The processor identifies a clonotype group in the data set using the parameter, identifies subclonotypes within the clonotype group (wherein each identified subclonotype comprises cells having identical V(D)J transcripts), and processes the data to define a visualization model that can display a compressed view of the identified clonotype group. The display renders a visualization of said data set according to said visualization model. The visualization displays the clonotype group by identified subclonotype.

Abstract: The invention relates to a method of preparing and using a library of template polynucleotides suitable for use as templates in solid-phase nucleic acid amplification and sequencing reactions to determine the methylation status of the cytosine bases in the library. In particular, the invention relates to a method of preparing and analysing a library of template polynucleotides suitable for methylation analysis.

Abstract: Described are computer-implemented methods, systems, and media for de novo phased diploid assembly of nucleic acid sequence data generated from a nucleic acid sample of an individual utilizing nucleic acid tags to preserve long-range sequence context for the individual such that a subset of short-read sequence data derived from a common starting sequence shares a common tag. The phased diploid assembly is achieved without alignment to a reference sequence derived from organisms other than the individual. The methods, systems, and media described are computer-resource efficient, allowing scale-up.

Abstract: Systems and methods for dynamically maintaining and utilizing walking profiles for time estimations in service scheduling are provided. A networked system detects usage of an application on a user device. In response to the detecting, the networked system accesses environmental condition data at a location associated with a user of the user device, whereby the environmental condition data comprises one or more environmental condition affecting the location. The networked system accesses walking pace data from the user device, whereby the walking pace data represents a current walking pace of the user and correlates the walking pace data with the environmental condition data. Using the correlated data, the networked system adjusts a parameter in a walking profile of the user. The walking profile is then used to determine time estimates that are used to schedule services that require walking by a user.

Abstract: Systems and methods for dynamically maintaining and utilizing walking profiles for time estimations in service scheduling are provided. A networked system detects usage of an application on a user device. In response to the detecting, the networked system accesses environmental condition data at a location associated with a user of the user device, whereby the environmental condition data comprises one or more environmental condition affecting the location. The networked system accesses walking pace data from the user device, whereby the walking pace data represents a current walking pace of the user and correlates the walking pace data with the environmental condition data. Using the correlated data, the networked system adjusts a parameter in a walking profile of the user. The walking profile is then used to determine time estimates that are used to schedule services that require walking by a user.

Abstract: The invention relates to a kit for preparing a library of template polynucleotides suitable for use as templates in solid-phase nucleic acid amplification and sequencing reactions to determine the methylation status of the cytosine bases in the library. In particular, the invention relates to a kit for preparing a library of template polynucleotides suitable for methylation analysis.

Abstract: The present disclosure provides methods and systems for producing full-length sequencing information of transcriptomes from single cells or from the bulk. Random ligation and circularization of barcoded or non-barcoded complementary deoxyribonucleic molecules can be used to provide a circular template for amplification and subsequent sequencing.

Abstract: Systems and methods for dynamically maintaining and utilizing walking profiles for time estimations in service scheduling are provided. A networked system detects usage of an application on a user device. In response to the detecting, the networked system accesses environmental condition data at a location associated with a user of the user device, whereby the environmental condition data comprises one or more environmental condition affecting the location. The networked system accesses walking pace data from the user device, whereby the walking pace data represents a current walking pace of the user and correlates the walking pace data with the environmental condition data. Using the correlated data, the networked system adjusts a parameter in a walking profile of the user. The walking profile is then used to determine time estimates that are used to schedule services that require walking by a user.

Abstract: Systems and methods for dynamically maintaining and utilizing walking profiles for time estimations in service scheduling are provided. A networked system detects usage of an application on a user device. In response to the detecting, the networked system accesses environmental condition data at a location associated with a user of the user device, whereby the environmental condition data comprises one or more environmental condition affecting the location. The networked system accesses walking pace data from the user device, whereby the walking pace data represents a current walking pace of the user and correlates the walking pace data with the environmental condition data. Using the correlated data, the networked system adjusts a parameter in a walking profile of the user. The walking profile is then used to determine time estimates that are used to schedule services that require walking by a user.

Abstract: Systems and methods for dynamically maintaining and utilizing walking profiles for time estimations in service scheduling are provided. A networked system detects usage of an application on a user device. In response to the detecting, the networked system accesses environmental condition data at a location associated with a user of the user device, whereby the environmental condition data comprises one or more environmental condition affecting the location. The networked system accesses walking pace data from the user device, whereby the walking pace data represents a current walking pace of the user and correlates the walking pace data with the environmental condition data. Using the correlated data, the networked system adjusts a parameter in a walking profile of the user. The walking profile is then used to determine time estimates that are used to schedule services that require walking by a user.

Abstract: A network computer system can receive location information indicating a current location of a user, upon detecting a user having arrived at an airport. based at least on the current location of the user, the network computer system can determine a time-to-reach pickup interval. Additionally, the network computer system can receive location information indicating a current location of each of a set of service providers. Based on at least on the current location of at least one service provider of the set of service providers, the network computer system can determine a request time interval. Moreover, based on the request time interval and the time-to-reach pickup interval, the network computer system can determine a computed request time indicating a time to trigger a service request.

Abstract: A system and method are provided which obtain a travel itinerary for a user, the itinerary identifying a trip, including a scheduled flight to a destination. A scheduled flight is monitored to detect when the scheduled flight arrives at the destination. Upon the scheduled flight arriving at an airport of the destination, one or more notifications are sent to a mobile computing device of the user. Information can be provided with the notifications for an on-demand ground transportation service. The information may include a location at the airport where the user can be picked up in connection with receiving the on-demand transportation service, and a timing indicator to indicate when the user should make a request to receive the on-demand transportation service based on a real-time determination of a number of available service providers in a vicinity of the airport.

Abstract: The invention relates to a kit for preparing a library of template polynucleotides suitable for use as templates in solid-phase nucleic acid amplification and sequencing reactions to determine the methylation status of the cytosine bases in the library. In particular, the invention relates to a kit for preparing a library of template polynucleotides suitable for methylation analysis.

Abstract: Methods form transistor structures that include, among other components, a substrate having an active region bordered by an isolation region, a gate insulator on the substrate, and a gate conductor on the gate insulator. First and second sections of the gate conductor are within the active region of the substrate, while a third section is in the isolation region of the substrate. The second section of the gate conductor tapers from the width of the first section to the width of the wider third section. The first section and the second section of the gate conductor have undercut regions where the corner of the gate conductor contacts the substrate. The third section of the gate conductor lacks the undercut regions. The gate insulator is relatively thicker in the undercut regions and is relatively thinner where the corner of the gate conductor lacks the undercut regions in the isolation region.

Abstract: Methods form transistor structures that include, among other components, a substrate having an active region bordered by an isolation region, a gate insulator on the substrate, and a gate conductor on the gate insulator. First and second sections of the gate conductor are within the active region of the substrate, while a third section is in the isolation region of the substrate. The second section of the gate conductor tapers from the width of the first section to the width of the wider third section. The first section and the second section of the gate conductor have undercut regions where the corner of the gate conductor contacts the substrate. The third section of the gate conductor lacks the undercut regions. The gate insulator is relatively thicker in the undercut regions and is relatively thinner where the corner of the gate conductor lacks the undercut regions in the isolation region.

Abstract: The invention relates to a method of preparing and using a library of template polynucleotides suitable for use as templates in solid-phase nucleic acid amplification and sequencing reactions to determine the methylation status of the cytosine bases in the library. In particular, the invention relates to a method of preparing and analyzing a library of template polynucleotides suitable for methylation analysis.

Abstract: Described are computer-implemented methods, systems, and media for de novo phased diploid assembly of nucleic acid sequence data generated from a nucleic acid sample of an individual utilizing nucleic acid tags to preserve long-range sequence context for the individual such that a subset of short-read sequence data derived from a common starting sequence shares a common tag. The phased diploid assembly is achieved without alignment to a reference sequence derived from organisms other than the individual. The methods, systems, and media described are computer-resource efficient, allowing scale-up.