Patents by Inventor David James Wright

David James Wright has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Strand displacement amplification using thermophilic enzymes
    Patent number: 5744311
    Abstract: Strand Displacement Amplification methods (thermophilic SDA) which can be performed over a broad temperature range (37.degree. C. to 70.degree. C.). The preferred temperature range for thermophilic SDA is 50.degree. C. to 70.degree. C. It has been found that certain thermophilic restriction endonucleases are capable of nicking the hemimodified restriction endonuclease recognition/cleavage site as required by SDA and dissociating from the site. It has further been found that certain thermophilic polymerases are capable of extending from the nick while displacing the downstream strand. Thermophilic SDA, because of reaction temperatures higher than previously possible with conventional SDA enzyme systems, has improved specificity and efficiency, reduced nonspecific background amplification, and potentially improved yields of amplification products.
    Type: Grant
    Filed: August 22, 1996
    Date of Patent: April 28, 1998
    Assignee: Becton, Dickinson and Company
    Inventors: Melinda S. Fraiser, Catherine A. Spargo, George Terrance Walker, Mark Van Cleve, David James Wright, Michael C. Little
  • Strand displacement amplification using thermophilic enzymes
    Patent number: 5648211
    Abstract: Strand Displacement Amplification methods (thermophilic SDA) which can be performed over a broad temperature range (37.degree. C. to 70.degree. C.). The preferred temperature range for thermophilic SDA is 50.degree. C. to 70.degree. C. It has been found that certain thermophilic restriction endonucleases are capable of nicking the hemimodified restriction endonuclease recognition/cleavage site as required by SDA and dissociating from the site. It has further been found that certain thermophilic polymerases are capable of extending from the nick while displacing the downstream strand. Thermophilic SDA, because of reaction temperatures higher than previously possible with conventional SDA enzyme systems, has improved specificity and efficiency, reduced nonspecific background amplification, and potentially improved yields of amplification products.
    Type: Grant
    Filed: April 18, 1994
    Date of Patent: July 15, 1997
    Assignee: Becton, Dickinson and Company
    Inventors: Melinda S. Fraiser, Catherine A. Spargo, George Terrance Walker, Mark Van Cleve, David James Wright, Michael C. Little