Abstract: A gene panel comprising genes regulated in mammalian skin in response to generation of a radio frequency current in a tissue volume of the mammalian skin sufficient to heat the tissue volume to a treatment temperature, wherein at least one gene is selected from Table 1 or Table 2 and at least one gene is selected from Table 3. Further there is a method for providing a benefit to mammalian skin, the benefit comprising inducing collagen formation and/or dermal remodeling in a dermal layer of the mammalian skin in the absence of a skin-damaging inflammatory cytokine response, the method comprising generating a radio frequency current in a tissue volume of the mammalian skin for a treatment cycle sufficient to heat the tissue volume to a treatment temperature while avoiding an upregulation in expression of genes listed in Table 3.

Abstract: A method for regulating the condition of mammalian skin by the steps of applying a first personal care composition to an area of skin where regulation is desired. The first personal care composition contains a gel composition. In another step energy is delivered to the dermis to heat collagen in the dermis such that the heated collagen in the dermis heats the epidermis and stratum corneum until the stratum corneum reaches an external temperature of from about 37° C. to about 48° C. The energy delivery to the dermis is then controlled to maintain the temperature of the stratum corneum in the range of from about 37° C. to about 48° C.

Abstract: The present disclosure relates to subtilisin protease conjugate comprising a protease moiety and one or more addition moieties. Each addition moiety is covalently attached to an epitope protection position of the protease moiety. The protease conjugates have decreased immunogenicity relative to a parent protease. The present disclosure further relates to cleaning and personal care compositions comprising the protease conjugates.

Abstract: A cosmetic method for providing improved skin hydration is provided comprising the steps of topically applying to the skin a protease enzyme, and simultaneously or sequentially, topically applying to the skin a skin care active selected from a vitamin B3 component, vitamin E acetate, panthenol and mixtures thereof.

Abstract: An oil in water emulsion are useful for treating skin or hair comprising:

Abstract: A cosmetic method for providing improved skin hydration is provided comprising the steps of topically applying to the skin a protease enzyme, and simultaneously or sequentially, topically applying to the skin a polyhydric alcohol. Also provided is a cosmetic method for providing improved skin hydration comprising topically applying to the skin a polyhydric alcohol, and simultaneously or sequentially, topically applying to the skin a protease enzyme.

Abstract: The present invention relates to enzyme variants having decreased immunogenicity relative to their corresponding wild-type enzymes. More particularly, the present invention relates to enzyme variants having a modified amino acid sequence of a wild-type amino acid sequence, wherein the modified amino acid sequence comprises a substitution of one or more amino acid positions with at least one D-amino acid. The invention further relates to mutant genes encoding such enzyme variants and cleaning and personal care compositions comprising such enzyme variants.

Abstract: Leave-on personal care compositions comprising a stabilized enzyme, at least 30% water, less than 5% of an earth alkali metal salt, and an enzyme inhibitor having an inhibition constant (Kl) from 10 nanomolar (nM) to 25,000 nM. Also, a method of providing a skin care benefits, e.g. skin feel and/or skin appearance, preferably skin moisturization, skin softness, and/or skin smoothness, comprising topically applying a safe and effective amount of the inventive compositions described herein to skin in need of such a skin care benefit. Further, a method of providing a consumer with a leave-on enzyme-containing personal care product, wherein the enzyme is inhibited and inactive on shelf, but is active on skin without the need for a separate re-activation step, preferably wherein the leave-on enzyme-containing personal care product comprises a one of the inventive compositions described herein.

Abstract: The present disclosure relates to subtilisin protease conjugate comprising a protease moiety and one or more addition moieties. Each addition moiety is covalently attached to a clip site protection position of the protease moiety, wherein the clip site protection positions are selected from 13, 14, 15, 16, 18, 19, 20, 21, 84, 85, 88, 158, 159, 160, 161, 162, 163, 164, 165, 170, 186, 191, 192, 193, 194, 196, 259, 260, 261, 262, and 274 corresponding to subtilisin BPN′. The protease conjugates have decreased immunogenicity relative to a parent protease. The present disclosure further relates to cleaning and personal care compositions comprising the protease conjugates.

Abstract: Protease conjugates such as subtilisin conjugates are provided comprising a protease moiety and one or more addition moieties wherein the protease moiety has a modified amino acid sequence of a parent amino acid sequence. The parent amino acid sequence comprises a first epitope region, a second epitope region and a third epitope region, and the modified amino acid sequence comprises a substitution by a substituting amino acid at one or more positions in one or more of the epitope regions. When a substitution occurs in the first epitope region, the substitution occurs at one or more positions corresponding to positions 70-84 of subtilisin BPN′. When a substitution occurs in the second epitope region, the substitution occurs at one or more positions corresponding to positions 103-126 of subtilisin BPN′. When a substitution occurs in the third epitope region, the substitution occurs at one or more positions corresponding to positions 217-252 of subtilisin BPN′.

Abstract: The present invention relates to personal care compositions comprising a water insoluble substrate, a plurality of active proteins, and a binding means, comprising a polymeric tether, permanently attaching each of the enzymes to the substrate wherein the personal care composition comprises from about 0.01 &mgr;g/cm2 to about 1000 &mgr;g/cm2 of the enzyme on the substrate.

Abstract: The present invention relates to skin care compositions comprising a skin conditioning agent and a topical carrier for the skin conditioning agent. The skin conditioning agent comprises certain nonocclusive liquid polyol carboxylic acid esters, wherein the liquid polyester has a complete melting point of less than about 30° C., and certain solid polyol carboxylic acid ester thickeners for the liquid polyester, wherein the solid polyester has a complete melting point of greater than about 30° C. These compositions provide excellent skin conditioning benefits.

Abstract: The present invention relates to personal care compositions comprising a water insoluble substrate, a plurality of Protease G enzymes, and a binding means, permanently attaching each of the enzymes to the substrate wherein the personal care compositions comprise from about 0.01 &mgr;g/cm2 to about 1000 &mgr;g/cm2 of the enzyme on the substrate.

Abstract: Disclosed is a stress stable lathering skin cleansing liquid composition comprising by weight parts of the liquid composition:(a) from about 0.5 parts to 10 parts of a crystalline, hydroxyl-containing stabilizer;(b) from about 1 part to about 80 parts of lipid skin moisturizing agent;(c) from about 1 part to about 30 parts of surfactant having a combined CMC equilibrium surface tension value of from 15 to 50;(d) water.Such stress stable lathering skin cleansing liquid compositions have a Lipid Deposition Value (LDV) of from about 5 to about 100. Such compositions are also stable for at least two weeks at 100.degree. F.

Abstract: The present invention relates to a stress stable lathering skin cleansing liquid composition comprising by weight parts of the liquid composition:(a) from about 0.5 parts to 10 parts of a stabilizer; for example trihydroxystearin;(b) from about 1 part to about 80 parts of lipid skin moisturizing agent;(c) from about 1 part to about 30 parts of surfactant having a combined CMC equilibrium surface tension value of from 15 to 50;(d) water;wherein said stress stable lathering skin cleansing liquid composition has a Lipid Deposition Value (LDV) of from about 5 to about 100 and wherein said composition is stable for at least two weeks at 100 F.

Abstract: A stress stable lathering skin cleansing liquid composition comprising by weight parts of the liquid composition:(a) from about 0.5 parts to 10 parts of a crystalline, hydroxy-containing stabilizer; for example trihydroxystearin;(b) from about 1 part to about 30 parts of lipid skin moisturizing agent;(c) from about 5 parts to about 30 parts of surfactant having a combined CMC equilibrium surface tension value of from 15 to 50;(d) water;wherein said stress stable lathering skin cleansing liquid composition has a Lipid Deposition Value (LDV) of from about 5 to about 100 and wherein the composition is stable for at least two weeks at 100.degree. F.