Abstract: Disclosed is a method of determining the presence and activity of interferon inhibitors in a test sample, preferably human blood serum. The method includes the steps of culturing cells which express an interferon-inducible antigen in the presence of an interferon and then adding an aliquot of the cultured cells to a test sample and to a control sample. The expression of the interferon-inducible antigen is then measured in both the test sample and in the control sample and these two values compared to determine the presence and activity of interferon inhibitors in the test sample.

Abstract: A method of treating cancer by the use of a multidrug chemotherapeutic regimen determined by in vitro pharmacosensitivity tests. A cell suspension is prepared from a tumor specimen obtained from the patient. The viable tumor cell count within the cell suspension is calculated. The volume of the cell suspension is then adjusted to obtain a base cell concentration by diluting the cell suspension with patient medium in proportion with the viable tumor cell count. A sample of the cell suspension is retained as a negative control sample. Drug samples are then prepared, each drug sample containing a mixture of cell suspension, patient medium, and a drug selected from several drugs, wherein each drug sample contains a different drug which is added to the drug sample in an aliquot amount proportional to the base cell concentration. The drug samples and negative control sample are then incubated. After incubation, the drug samples and negative control sample are stained with a DNA intercalating dye.