Abstract: In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of at least one agent that generates a ligatable terminal 5? phosphate group by removing an adenylate group from a terminal 5? phosphate of a nucleic acid. In some embodiments, an aprataxin enzyme can catalyze removal of an adenylate group from a terminal 5? phosphate of a nucleic acid. In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of an aprataxin enzyme under conditions suitable for ligating nucleic acid ends.

Abstract: The invention presents a harness assembly for lifting and supporting an animal in mobility. The assembly comprises a front and rear harness sections releasably attached by a lifting leash. Both the front and rear harness sections can be used independently. Both the front and rear harness sections are comprised of upper and lower sections releasably attached to one another. The rear harness section lifts the animal from both the hips and waist resulting in less stress than other devices.

Abstract: Disclosed are systems, methods, and articles for determining compatibility of a mobile application and operating system on a mobile device. In some aspects, a method includes receiving one or more data values from a mobile device having a mobile medical software application installed thereon, the data value(s) characterizing a version of the software application, a version of an operating system installed on the mobile device, and one or more attributes of the mobile device; determining whether the mobile medical software application is compatible with the operating system by at least comparing the received data value(s) to one or more test values in a configuration file; and sending a message to the mobile device based on the determining, the message causing the software application to operate in one or more of a normal mode, a safe mode, and a non-operational mode.

Abstract: A computer-implemented schema-independent method of modeling data from a plurality of diverse sources. A server transmits to a client a blueprint for visualizing and interacting with data, wherein the blueprint defines an application, for visualizing and interacting with data that runs on the client. The application may operate on the client within a web browser and may include program code or scripts that operate within the web browser and transmit data and commands to and from the server. In response to receiving a data fetch message from the application running on a client computer, the server receives data from a selected one of a plurality of domains. The server then transforms the received data into a semantic data format. The transformed data is then stored by the server as a first data set. The first data set can then be transmitted to the client computer for further processing and visualization by the application on the client using the blueprint.

Abstract: A taco shell is produced using a rotary cutter including a shaft, an outer blade extending radially outward from the shaft and a first inner blade extending radially outward from the shaft. The outer blade defines an interior area, enclosed by the outer blade, and an exterior area. The first inner blade is located in the interior area and includes a plurality of teeth. A dough piece is cut from a dough sheet with the outer blade, and a first arcuate line of depressions is created in the dough piece with the plurality of teeth. The dough piece is folded to form a shaped dough piece, and the shaped dough piece is baked to produce a hard taco shell.

Abstract: Methods, assays, compositions and kits for the ligation of short polynucleotides are presented herein. The short polynucleotides are optionally no more than 7 nucleotides in length, and can be as short as 3 or 4 nucleotides in length. The ligation is optionally performed by CV ligase.

Abstract: Methods for analyzing raw electropherogram data are disclosed. Some methods includes extracting color data as a function of time or position from the raw electropherogram darta, selecting from the electropherogram one or more peaks that contain color data for a first dye and substantially no color data from other dyes used in electrophoresis. The method also includes determining the color spectrum of the first dye, and using the color spectrum of the first dye to deconvolve the color data of the raw electropherogram data to separate the contributions of each of the dyes to the raw electropherogram data. Systems and apparatus for producing electropherograms are also disclosed.

Abstract: Methods and apparatuses for automatically switching different aspiration levels to an ocular probe are disclosed herein. The probe may be a phacoemulsification probe. A first aspiration level, supplied by a first pump, may be applied to the probe simultaneously with ultrasonic energy. A second aspiration level, supplied by a second pump, may be automatically switched from the first aspiration level. Control feed back of the pumps may be varied according to set thresholds.

Abstract: A system for performing an ocular surgical procedure is provided. The system includes a multiple frequency signal source, a configurable tuned output filter connected to the multiple frequency signal source, and a multiple frequency ultrasonic handpiece. The multiple frequency signal source operates at a first frequency and is configured to drive the configurable filter and the multiple frequency ultrasonic handpiece at the first frequency. The multiple frequency signal source operates at a second frequency and is configured to drive the configurable filter and the multiple frequency ultrasonic handpiece at the second frequency, and the design addresses third harmonic frequency issues for the multiple frequency ultrasonic handpiece. Switchable passive components, such as inductors, resistors, and/or capacitors may be employed in the configurable tuned output circuit, or alternately multiple similar circuits may be employed. Alternately, a multi-tap transformer may be provided.

Abstract: Provided are antibodies and antigen-binding fragments thereof that specifically bind to human neuropilin-2 (NRP2) polypeptides, including those that modulate binding interactions between human NRP2 and at least one NRP2 ligand, for example, human histidyl-tRNA synthetase (HRS), and which thereby modulate subsequent NRP2-mediated downstream signaling events, including related therapeutic compositions and methods for modulating NRP2 activity and treating diseases such as NRP2-associated diseases

Abstract: Systems and methods for automated and direct network positioning are disclosed herein. The system can include memory that can include a content library database and a structure sub-database. The system can include at least one server. The at least one server can: receive a data packet previously unassociated with the hierarchy in the structure sub-database; identify one of the plurality of positions within the hierarchy for the data packet; receive user information; present a series of assessment data packets to the user; receive a response from the user subsequent to presentation of each of the assessment data packets; evaluate the received responses; adjust a location of the user within the hierarchy based on the evaluating of the received responses; and present a content data packet to the user.

Abstract: A hot water heater appliance includes a boiler, a companion water heater and a controller. The controller is configured to control the water heater appliance in response to a scheduled plurality of performance modes based on a preset schedule. A first performance mode is scheduled to be performed at an expected low usage time and a second performance mode is scheduled to be performed at an expected high usage time relative to the expected low usage time.

Abstract: Systems and methods for automated content delivery and evaluation are disclosed herein. The system can include a memory. The memory can include a content library database including a plurality of problems and data for stepwise evaluation of each of the plurality of problems. The system can include at least one server. The at least one server can automatically decompose a content item into a plurality of potential steps and associate attributes with the potential steps. The at least one server can receive a response from a user for the content item, identify steps in the received response, and select a next action based the identified steps of the received response.

Abstract: Systems and methods for automated content delivery and evaluation are disclosed herein. The system can include a memory. The memory can include a content library database including a plurality of problems and data for stepwise evaluation of each of the plurality of problems. The system can include at least one server. The at least one server can automatically decompose a content item into a plurality of potential steps and associate attributes with the potential steps. The at least one server can receive a response from a user for the content item, identify steps in the received response, and select a next action based the identified steps of the received response.

Abstract: Provided herein is a fluidic cartridge having a body comprising a malleable material and a layer comprising a deformable material bonded to a surface of the body that seals one or more fluidic channels that communicate with one or more valve bodies formed in a surface of the body. The valve can be closed by applying pressure to the deformable material sufficient to crush and close off a fluidic channel in the body. Also provided are a cartridge interface configured to engage the cartridge. Also provided is a system including a cartridge interface and methods of using the cartridge and system.

Abstract: This disclosure provides, among other things, a cartridge comprising: (a) a cartridge body comprising a malleable material and having, disposed on a surface of the body, at least one valve body comprising a valve inlet and a valve outlet, each fluidically connected to a fluidic channel; and (b) a layer comprising a deformable material bonded to a surface of the cartridge body and sealing the at least one valve body at points of attachment, thereby forming at least one valve; wherein the at least one valve body is depressed in the cartridge body relative to the points of attachment and wherein the deformable material covering the at least one valve body retains sufficient elasticity after deformation such that in a ground state the valve is open.