Patents by Inventor David Kleinfeld
David Kleinfeld has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11821826Abstract: The present disclosure relates to systems and methods for facing a tissue block. In some embodiments, a method is provided for facing a tissue block that includes imaging a tissue block to generate imaging data of the tissue block, the tissue block comprising a tissue sample embedded in an embedding material, estimating, based on the imaging data, a depth profile of the tissue block, wherein the depth profile comprises a thickness of the embedding material to be removed to expose the tissue sample to a pre-determined criteria, and removing the thickness of the embedding material to expose the tissue to the pre-determined criteria.Type: GrantFiled: June 29, 2022Date of Patent: November 21, 2023Assignee: Clarapath, Inc.Inventors: Partha P. Mitra, Charles Cantor, Baris Yagci, David Kleinfeld, Cong Zhang, Steven Smith
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Publication number: 20230126618Abstract: The present disclosure also relates to systems and methods for quality control in histology systems. In some embodiments, a method is provided that includes receiving a tissue block comprising a tissue sample embedded in an embedding material, imaging the tissue block to create a first imaging data of the tissue sample in a tissue section on the tissue block, removing the tissue section from the tissue block, the tissue section comprising a part of the tissue sample, imaging the tissue section to create a second imaging data of the tissue sample in the tissue section, and comparing the first imaging data to the second imaging data to confirm correspondence in the tissue sample in the first imaging data and the second imaging data based on one or more quality control parameters.Type: ApplicationFiled: December 23, 2022Publication date: April 27, 2023Applicant: Clarapath, Inc.Inventors: Partha P. Mitra, Charles Cantor, Baris Yagci, David Kleinfeld, Cong Zhang
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Patent number: 11609162Abstract: The present disclosure also relates to systems and methods for quality control in histology systems. In some embodiments, a method is provided that includes receiving a tissue block comprising a tissue sample embedded in an embedding material, imaging the tissue block to create a first imaging data of the tissue sample in a tissue section on the tissue block, removing the tissue section from the tissue block, the tissue section comprising a part of the tissue sample, imaging the tissue section to create a second imaging data of the tissue sample in the tissue section, and comparing the first imaging data to the second imaging data to confirm correspondence in the tissue sample in the first imaging data and the second imaging data based on one or more quality control parameters.Type: GrantFiled: October 20, 2021Date of Patent: March 21, 2023Assignee: Clarapath, Inc.Inventors: Partha P. Mitra, Charles Cantor, Baris Yagci, David Kleinfeld, Cong Zhang
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Publication number: 20220349788Abstract: The present disclosure relates to systems and methods for facing a tissue block. In some embodiments, a method is provided for facing a tissue block that includes imaging a tissue block to generate imaging data of the tissue block, the tissue block comprising a tissue sample embedded in an embedding material, estimating, based on the imaging data, a depth profile of the tissue block, wherein the depth profile comprises a thickness of the embedding material to be removed to expose the tissue sample to a pre-determined criteria, and removing the thickness of the embedding material to expose the tissue to the pre-determined criteria.Type: ApplicationFiled: June 29, 2022Publication date: November 3, 2022Applicant: Clarapath, Inc.Inventors: Partha P. Mitra, Charles Cantor, Baris Yagci, David Kleinfeld, Cong Zhang, Steven Smith
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Patent number: 11467071Abstract: The present disclosure relates to systems and methods for facing a tissue block. In some embodiments, a method is provided for facing a tissue block that includes imaging a tissue block to generate imaging data of the tissue block, the tissue block comprising a tissue sample embedded in an embedding material, estimating, based on the imaging data, a depth profile of the tissue block, wherein the depth profile comprises a thickness of the embedding material to be removed to expose the tissue sample to a pre-determined criteria, and removing the thickness of the embedding material to expose the tissue to the pre-determined criteria.Type: GrantFiled: October 22, 2021Date of Patent: October 11, 2022Assignee: Clarapath, Inc.Inventors: Partha P. Mitra, Charles Cantor, Baris Yagci, David Kleinfeld, Cong Zhang, Steven Smith
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Publication number: 20220042887Abstract: The present disclosure relates to systems and methods for facing a tissue block. In some embodiments, a method is provided for facing a tissue block that includes imaging a tissue block to generate imaging data of the tissue block, the tissue block comprising a tissue sample embedded in an embedding material, estimating, based on the imaging data, a depth profile of the tissue block, wherein the depth profile comprises a thickness of the embedding material to be removed to expose the tissue sample to a pre-determined criteria, and removing the thickness of the embedding material to expose the tissue to the pre-determined criteria.Type: ApplicationFiled: October 22, 2021Publication date: February 10, 2022Inventors: Partha P. Mitra, Charles Cantor, Baris Yagci, David Kleinfeld, Cong Zhang, Steven Smith
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Publication number: 20220034768Abstract: The present disclosure also relates to systems and methods for quality control in histology systems. In some embodiments, a method is provided that includes receiving a tissue block comprising a tissue sample embedded in an embedding material, imaging the tissue block to create a first imaging data of the tissue sample in a tissue section on the tissue block, removing the tissue section from the tissue block, the tissue section comprising a part of the tissue sample, imaging the tissue section to create a second imaging data of the tissue sample in the tissue section, and comparing the first imaging data to the second imaging data to confirm correspondence in the tissue sample in the first imaging data and the second imaging data based on one or more quality control parameters.Type: ApplicationFiled: October 20, 2021Publication date: February 3, 2022Inventors: Partha P. Mitra, Charles Cantor, Baris Yagci, David Kleinfeld, Cong Zhang
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Publication number: 20210262907Abstract: The present disclosure relates to systems and methods for facing a tissue block. In some embodiments, a method is provided for facing a tissue block that includes imaging a tissue block to generate imaging data of the tissue block, the tissue block comprising a tissue sample embedded in an embedding material, estimating, based on the imaging data, a depth profile of the tissue block, wherein the depth profile comprises a thickness of the embedding material to be removed to expose the tissue sample to a pre-determined criteria, and removing the thickness of the embedding material to expose the tissue to the pre-determined criteria.Type: ApplicationFiled: February 22, 2021Publication date: August 26, 2021Inventors: Partha P. Mitra, Charles Cantor, Baris Yagci, David Kleinfeld, Cong Zhang, Steven Smith
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Publication number: 20210263055Abstract: The present disclosure also relates to systems and methods for quality control in histology systems. In some embodiments, a method is provided that includes receiving a tissue block comprising a tissue sample embedded in an embedding material, imaging the tissue block to create a first imaging data of the tissue sample in a tissue section on the tissue block, removing the tissue section from the tissue block, the tissue section comprising a part of the tissue sample, imaging the tissue section to create a second imaging data of the tissue sample in the tissue section, and comparing the first imaging data to the second imaging data to confirm correspondence in the tissue sample in the first imaging data and the second imaging data based on one or more quality control parameters.Type: ApplicationFiled: February 22, 2021Publication date: August 26, 2021Inventors: Partha P. Mitra, Charles Cantor, Baris Yagci, David Kleinfeld, Cong Zhang
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Patent number: 9545689Abstract: Temporal focusing of spatially chirped femtosecond laser pulses overcomes previous limitations for ablating high aspect ratio features with low numerical aperture (NA) beams. Simultaneous spatial and temporal focusing reduces nonlinear interactions, such as self-focusing, prior to the focal plane so that deep (˜1 mm) features with parallel sidewalls are ablated at high material removal rates.Type: GrantFiled: December 9, 2013Date of Patent: January 17, 2017Assignee: Colorado School of MinesInventors: Jeffrey Squier, Dawn Vitek, Charles Durfee, Daniel Adams, David Kleinfeld
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Publication number: 20140103018Abstract: Temporal focusing of spatially chirped femtosecond laser pulses overcomes previous limitations for ablating high aspect ratio features with low numerical aperture (NA) beams. Simultaneous spatial and temporal focusing reduces nonlinear interactions, such as self-focusing, prior to the focal plane so that deep (˜1 mm) features with parallel sidewalls are ablated at high material removal rates.Type: ApplicationFiled: December 9, 2013Publication date: April 17, 2014Applicant: Colorado School of MinesInventors: Jeffrey Squier, Dawn Vitek, Charles Durfee, Daniel Adams, David Kleinfeld
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Patent number: 8669488Abstract: Temporal focusing of spatially chirped femtosecond laser pulses overcomes previous limitations for ablating high aspect ratio features with low numerical aperture (NA) beams. Simultaneous spatial and temporal focusing reduces nonlinear interactions, such as self-focusing, prior to the focal plane so that deep (˜1 mm) features with parallel sidewalls are ablated at high material removal rates.Type: GrantFiled: March 30, 2011Date of Patent: March 11, 2014Assignee: Colorado School of MinesInventors: Jeffrey Squier, Dawn Vitek, Charles Durfee, Daniel Adams, David Kleinfeld
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Patent number: 8663604Abstract: A method is provided for in vivo detection of a biochemical substance in an animal by culturing neurofluocytes that stably express a receptor of the biochemical substances by transfecting cells with cDNA of the receptor and a tag that will emit a detectable energy in the presence of the biochemical substance, implanting the neurofluocyte into the animal's brain; and detecting the energy emission of the tag. In a first embodiment, the biochemical substance is a neurotransmitter, the tag is a fluophore, and the step of detecting includes forming an opening in the animal's skull and optically detecting fluorescent emissions using a two-photon laser scanning microscope. Multiple biochemical substances can be simultaneously detected by culturing neurofluocytes that express different receptors and have different fluophor tags that produce fluorescent signals at distinguishable wavelengths.Type: GrantFiled: September 4, 2008Date of Patent: March 4, 2014Assignee: The Regents of the University of CaliforniaInventors: Quoc-Thang Nguyen, David Kleinfeld, Lee F. Schroeder
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Publication number: 20110297651Abstract: Temporal focusing of spatially chirped femtosecond laser pulses overcomes previous limitations for ablating high aspect ratio features with low numerical aperture (NA) beams. Simultaneous spatial and temporal focusing reduces nonlinear interactions, such as self-focusing, prior to the focal plane so that deep (˜1 mm) features with parallel sidewalls are ablated at high material removal rates.Type: ApplicationFiled: March 30, 2011Publication date: December 8, 2011Inventors: Jeffrey Squier, Dawn Vitek, David Kleinfeld, Daniel Adams, Charles Durfee
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Publication number: 20100278746Abstract: A method is provided for in vivo detection of a biochemical substance in an animal by culturing neurofluocytes that stably express a receptor of the biochemical substances by transfecting cells with cDNA of the receptor and a tag that will emit a detectable energy in the presence of the biochemical substance, implanting the neurofluocyte into the animal's brain; and detecting the energy emission of the tag. In a first embodiment, the biochemical substance is a neurotransmitter, the tag is a fluophore, and the step of detecting includes forming an opening in the animal's skull and optically detecting fluorescent emissions using a two-photon laser scanning microscope. Multiple biochemical substances can be simultaneously detected by culturing neurofluocytes that express different receptors and have different fluophor tags that produce fluorescent signals at distinguishable wavelengths.Type: ApplicationFiled: September 4, 2008Publication date: November 4, 2010Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Quoc-Thang Nguyen, David Kleinfeld, Lee F. Schroeder
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Publication number: 20070299331Abstract: Ultrashort laser pulses are used to induce photodisruptive breakdown in vasculature in an animal to controllably produce hemorrhage, thrombosis or breach of blood-brain barrier in individual, specifically targeted blood vessels. Damage is limited to the targeted vessels such that neighboring vessels exhibit no signs of vascular damage, including vessels directly above or below the targeted vessel. Ultrashort laser pulses of lower energy are also used to observe and quantify the baseline and altered states of blood flow. Observation and measurement may be performed (1) by TPLSM, OCT or other known techniques, providing a real-time, in vivo model for the dynamics and effects of vascular injury.Type: ApplicationFiled: July 13, 2007Publication date: December 27, 2007Inventors: Beth FRIEDMAN, David KLEINFELD, Patrick LYDEN, Nozomi NISHIMURA, Christopher SCHAFFER, Lee SCHROEDER, Philbert TSAI
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Patent number: 7258687Abstract: Ultrashort laser pulses are used to induce photodisruptive breakdown in vasculature in an animal to controllably produce hemorrhage, thrombosis or breach of the blood-brain barrier in individual, specifically-targeted blood vessels. Damage is limited to the targeted vessels such that neighboring vessels exhibit no signs of vascular damage, including vessels directly above and directly below the targeted vessel. Ultrashort laser pulses of lower energy are also used to observe and quantify the baseline and altered states of blood flow. Observation and measurement may be performed by TPLSM, OCT or other known techniques, providing a real-time, in vivo model for the dynamics and effects of vascular injury.Type: GrantFiled: December 11, 2003Date of Patent: August 21, 2007Assignee: The Regents of the University of CaliforniaInventors: Beth Friedman, David Kleinfeld, Patrick D. Lyden, Nozomi Nishimura, Christopher B. Schaffer, Lee Frederick Schroeder, Philbert Tsai
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Publication number: 20060142746Abstract: Ultrashort laser pulses are used to induce photodisruptive breakdown in vasculature in an animal to controllably produce hemorrhage, thrombosis or breach of the blood-brain barrier in individual, specifically-targeted blood vessels. Damage is limited to the targeted vessels such that neighboring vessels exhibit no signs of vascular damage, including vessels directly above and directly below the targeted vessel. Ultrashort laser pulses of lower energy are also used to observe and quantify the baseline and altered states of blood flow. Observation and measurement may be performed by TPLSM, OCT or other known techniques, providing a real-time, in vivo model for the dynamics and effects of vascular injury.Type: ApplicationFiled: December 11, 2003Publication date: June 29, 2006Inventors: Beth Friedman, David Kleinfeld, Patrick Lyden, Nozomi Nishimura, Christopher Schaffer, Lee Schroeder, Philbert Tsai
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Publication number: 20050035305Abstract: Femtosecond laser pulses are used to iteratively cut and image fixed as well as exsanguinated fresh tissue. Such images help to automate three-dimensional histological analysis of biological tissue. Cuts are accomplished with approximately 0.3 to 100 microJoule pulses to ablate tissue with one-micrometer precision. Permeability, immunoreactivity, and optical clarity of the remaining tissue is retained after pulsed laser cutting. Samples from transgenic mice that express fluorescent proteins retained their fluorescence to within micrometers of the cut surface.Type: ApplicationFiled: July 8, 2004Publication date: February 17, 2005Inventors: David Kleinfeld, Jeffrey Squier, Philbert Tsai
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Patent number: 4752906Abstract: A sequence generator employing a neural network having its output coupled to at least one plurality of delay elements. The delayed outputs are fed back to an input interconnection network, wherein they contribute to the next state transition through an appropriate combination of interconnections.Type: GrantFiled: December 16, 1986Date of Patent: June 21, 1988Assignee: American Telephone & Telegraph Company, AT&T Bell LaboratoriesInventor: David Kleinfeld