Abstract: Described is a composition with low toxicity comprising an amphipathic compound and a polycation, useful for delivering siRNA to a cell. The composition may be used in the process of delivering a siRNA to an animal cell, or more particularly a mammal cell, in a multi-well format.

Abstract: A process is provided for inhibition of specific gene expression in an animal cell by delivering a combination of RNA function inhibitors. Using a combination of inhibitors results in improved efficacy of gene expression inhibition. The process can be used to reduce gene expression in cells in vitro and in vivo.

Abstract: Compositions are provided for delivery of polynucleotides to cells for the purpose of inhibiting gene expression. Antisense polynucleotide-containing complexes are described. The salt and serum stability and small size of the complexes permits delivery to cells in vitro and in vivo.

Abstract: Described is a process for delivering an inhibitor directed against an expressible nucleic acid sequence in a mammal to inhibit RNA function. An RNA function inhibiting sequence that is specific to the expressible nucleic acid sequence in the mammal is made and inserted into a blood vessel in the mammal. The inhibitor is delivered to a cell wherein expression of the nucleic acid sequence is inhibited.

Abstract: Thermal conductivity of glassware forming blank molds and blow molds of Ni-Resist ductile iron is selectively controlled by formation of compacted graphite in the mold microstructure during preparation of the melt and casting of the mold bodies. Specifically, with a Type D5 Ni-Resist ductile iron according to ASTM-A439-84, compacted graphite is selectively formed in the cast microstructure of the mold body by reducing the magnesium and sulphur concentrations in the iron composition to the range of 0.01 to 0.04 wt % magnesium and 0.00 to 0.01 wt % sulphur, and adding titanium to the iron composition in the range of 0.10 to 0.25 wt % titanium. Whereas formation of compacted graphite in the cast microstructure is normally considered to be undesirable for glassware forming molds, it has been found that formation of a small but appreciable amount of graphite provides the opportunity selectively to tailor the thermal conductivity characteristics of the mold body.

Abstract: A glassware forming mold that includes a body of heat conductive construction having a central portion with a forming surface for shaping molten glass and a peripheral portion spaced radially outwardly of the central portion. A plurality of coolant passages extend in a spaced array through the peripheral portion of the mold body, and liquid coolant is directed through such passages for extracting heat from the body by conduction from the forming surface. A plurality of openings extend axially into the body radially between at least some of the liquid coolant passages and the forming surface for retarding heat transfer from the forming surface to liquid coolant in the passages.

Abstract: A porous spacer incorporates a vaporizable corrosion inhibitor. The spacer is designed to be placed on a leader pin between the members of a mold or die set. The spacer is compressed between the members when the latter are stored. The spacer releases the inhibitor, which prevents corrosion during the storage period. The spacer serves to maintain a vapor flow space between the members.

Abstract: A position sensing switch combined with a slide retainer for injection molds of the type having at least a portion of the molding cavity formed in slides which are slidingly mounted on one of a pair of mold halves such that the slides advance between open and closed positions laterally relative to the mold halves as the mold halves are similarly advanced between open and closed positions. The slide retainer comprises a locking member, such as a spring loaded plunger secured on the slide, and a receiver, such as a detent formed on a slide plate of the mold half to which the slide is slidably connected. The detent is formed in a cover plate extending over a cavity extending into the slide plate and in which the switch is positioned.

Abstract: Specially-shaped blocks of foam for use underground as aggregate. Because of protuberances on the faces of the blocks, the blocks fit closely together in only a small number of ways. In filling a volume, the overwhelmingly more likely arrangements provide a reasonably certain percentage void. Thus, the blocks can be transported to an application arranged without any significant void, and then used to fill a volume, providing a reasonably well known percentage void and compressive strength. Use of these foam blocks can provide thermal insulation, cushioning and various combinations of compressive strength and void, depending on the size and shape of the blocks and the size and shape of the protuberances relative to the blocks.

Abstract: An angle spindle attachment for a vertically movable spindle unit is provided for rotating a cutting tool in a generally horizontal orientation. The attachment includes a spindle assembly having a drive train that is mechanically independent from the drive train of the vertically movable spindle unit, and a connector block for detachably connecting the spindle assembly at a transverse angle with respect to the vertically movable spindle unit. The connector block may include an angular adjustment mechanism that allows the spindle assembly to be oriented at an angle above or below the horizontal, as well as a rotational adjustment assembly that allows the spindle assembly to be pivotally adjusted about the axis of rotation of the vertically movable spindle unit.

Abstract: A cooler for storing beverage containers on ice, includes an outer container and a buoyant inner container assembly which is dimensioned to slide vertically within the outer container. A container recess is provided in the upper surface of the inner container assembly to hold beverage containers and ice, and the assembly includes a drain for the liquid formed by melting ice from the recess into the outer container. The inner container assembly has a buoyancy which will float it upon the liquid which is drained into the outer container.

Abstract: A collet chuck 1 having a chuck body 3, a collet 11 disposed in the body 3, a nose ring 23 for radially compressing the collet 11, and a locknut 30 for axially moving the nose ring 23 into compressive engagement with the collet 11 is provided with a bearing assembly 45 disposed between the nose ring 23 and the collet 11 which loads compressive forces in a direction which is advantageously parallel to the axis of rotation A of the chuck. Both the locknut 30 and the nose ring 23 include flange portions 27, 43 that confront each other with opposing surfaces 47, 49 that are complementary in shape to the spherical profile of the ball bearings 50 and which traverse a line L parallel to the axis of rotation A of the chuck. The collet chuck 1 further includes O-rings 61, 65 disposed between the nose ring 23 and the locknut 30both in front of and behind the ball bearings 50 for sealing the bearing assembly 45 against dust and debris and retaining lubricant.

Abstract: A toolholder 1 of the type including a collet 5 integrally formed with a shank 3 is provided with an interface between a locknut 7 and collet 13 which substantially reduces the torque required to turn the locknut 7 into a position which radially collapses the collet segments 17. In this interface, one of either the inner locknut surface 23 or the outer surfaces of the collet segments 17 includes an engaging portion 56 for wedgingly flexing the collet segments 17 inwardly when the locknut is turned, and a non-engaging portion 58 disposed between the engaging portion 56 and the shank mounted ends of the collet segments 17. The non-engaging portion 58 reduces the torque required to turn the locknut 7 by reducing the binding forces that would otherwise occur between the ends of the collet segments 17 and the inner locknut surface 23.

Abstract: A water filter provides good tasting, potable water without chemical biocides. A water filter utilizing activated carbon to rid the water of undesirable chemicals and the like has dolomite added to the activated carbon. The dolomite acts as a nutrient for harmless bacteria and thereby promotes the growth of the harmless bacteria to the exclusion of harmful bacteria. Dolomite produces a slight alkalinity for improved taste, provides magnesium and calcium as dietary supplements, and encourages the growth of bacteria that produce no undesirable metabolites.

Abstract: An insulating panel for buildings has an insulating core with one face imprinted with a grid pattern and a multiplicity of rectilinear lines radiating from one corner and defining increasing included angles relative to the base side of the panel which corresponds to pitch lines of roofs. The pitch line of a roof may be determined, and then the equivalent angular line located on the printed face. The panel may be cut along this line to abut closely the structural member of the roof extending at that angle. Vertical and horizontal measurements of the space in which the panel is to be fitted can be readily transposed to the grid pattern for making perpendicular or horizontal cuts therealong. The panel is conveniently formed by imprinting a facing sheet member and securing it to a synthetic resin core member.

Abstract: A chuck assembly and collet for retaining a commercially-available large-diameter, high-torque end mill (or similar) tool against axial pullout and against radial (or rotational) movement with respect to the assembly. The tool includes two axially spaced flat surfaces along the shank, one of which is coupled to the collet to prevent axial pullout and the other of which is coupled to the chuck to prevent rotation with respect to the chuck. The coupling to the chuck is accomplished by a ballclamp screw which extends through an aperture in the collet to the flat surface on the tool. The ballclamp screw has a flattened ball that is pivotally mounted in the screw's engagement and the pivotal mounting accommodates a few degree misalignment between the shank's flat surface and the screw without significantly affecting the coupling therebetween.

Abstract: A Friedmann analyser wtih an arrangement of fixed and rotatable shutters for displaying different patterns of light spots to a subject has a screen for displaying to the operator each light spot pattern selected by illumination of a series of light-emitting elements at fixed positions over the screen area. The light-emitting elements are actuated by a fixed array of infra-red emitting and receiving elements that direct radiation onto a binary-coded reflective pattern displaceable with the rotatable shutter and sense the radiation reflected by said pattern so that with the displacement of the shutter different reflected code signals are sensed associated with respective shutter light patterns displayed to the subject.

Abstract: A chuck assembly and collet for retaining a commercially-available large-diameter, high-torque end mill (or similar) tool against axial pullout and against radial (or rotational) movement with respect to the assembly. The tool includes two axially spaced flat surfaces along the shank, one of which is coupled to the collet to prevent axial pullout and the other of which is coupled to the chuck to prevent rotation with respect to the chuck. The coupling to the chuck is accomplished by a ballclamp screw which extends through an aperture in the collet to the flat surface on the tool. The ballclamp screw has a flattened ball that is pivotally mounted in the screw's engagement and the pivotal mounting accommodates a few degrees misalignment between the shank's flat surface and the screw without significantly affecting the coupling therebetween.

Abstract: A machine for cutting grooves in foam workpieces includes a plurality of vertically spaced U-shaped cutting wires mounted on pairs of mounting posts. One leg of each U-shaped wire is mounted on one post, while the other leg is mounted on the other post. The posts are mounted on a frame so as to be adjustable in spacing and vertical position with respect to each other, so the widths of the grooves can thereby be adjusted. Horizontal slice-cutting wires are provided behind the groove-cutting wires so that grooves and slices can be cut simultaneously. The slice-cutting wires are aligned at an angle with the vertical so that each slice-cutting wire exits the workpiece before all those beneath it. One version produces tongue-and-groove sheet, while ship-lap sheets are produced by the other version.

Abstract: A machine for cutting grooves in foam workpieces includes a plurality of vertically spaced U-shaped cutting wires mounted on pairs of mounting posts. One leg of each U-shaped wire is mounted on one post, while the other leg is mounted on the other post. The posts are mounted on a frame so as to be adjustable in spacing and vertical position with respect to each other, so the widths of the grooves can thereby be adjusted. Horizontal slice-cutting wires are provided behind the groove-cutting wires so that grooves and slices can be cut simultaneously. The slice-cutting wires are aligned at an angle with the vertical so that each slice-cutting wire exits the workpiece before all those beneath it. One version produces tongue-and-groove sheet, while ship-lap sheets are produced by the other version.