Patents by Inventor David LARA - ASTIASO

David LARA - ASTIASO has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • CRISP-seq, an integrated method for massively parallel single cell RNA-seq and CRISPR pooled screens
    Patent number: 11485971
    Abstract: An expression construct is disclosed which comprises: (i) a DNA sequence which encodes at least one guide RNA (gRNA) operatively linked to a transcriptional regulatory sequence so as to allow expression of the gRNA in a target cell; (ii) a barcode sequence for identification of the at least one gRNA operatively linked to a transcriptional regulatory sequence so as to allow expression of the barcode sequence in the target cell.
    Type: Grant
    Filed: September 14, 2017
    Date of Patent: November 1, 2022
    Assignee: Yeda Research and Development Co. Ltd.
    Inventors: Ido Amit, Diego Jaitin, David Lara-Astiaso, Assaf Weiner, Ido Yofe
  • Methods and kits for analyzing DNA binding moieties attached to DNA
    Patent number: 10590467
    Abstract: A method of analyzing DNA which, in a cell, is bound to a DNA binding moiety, the method comprising: (a) obtaining at least two samples of complexes of DNA bound to a DNA binding moiety; (b) isolating the complexes on a solid support; (c) labeling the DNA of the complexes, wherein the labeling distinguishes between the complexes of the first of the at least two samples and the complexes of the second of the at least two samples; (d) pooling the at least two samples of complexes; and (e) isolating the complexes using an agent which specifically binds to the DNA binding moiety; and (f) analyzing the DNA of the complexes.
    Type: Grant
    Filed: April 16, 2015
    Date of Patent: March 17, 2020
    Assignee: Yeda Research and Development Co. Ltd.
    Inventors: Ido Amit, David Lara-Astiaso, Meital Gury
  • METHODS AND KITS FOR ANALYZING DNA BINDING MOIETIES ATTACHED TO DNA
    Publication number: 20190203270
    Abstract: Methods of analyzing DNA molecules in a cell sample are provided. The DNA molecules have DNA binding moiety signatures which are defined by at least two non-identical DNA binding moieties. Kits for analyzing the DNA are also provided.
    Type: Application
    Filed: July 24, 2016
    Publication date: July 4, 2019
    Applicant: Yeda Research and Development Co., Ltd.
    Inventors: Ido AMIT, David LARA-ASTIASO, Meital GURY
  • CRISP-SEQ, AN INTEGRATED METHOD FOR MASSIVELY PARALLEL SINGLE CELL RNA-SEQ AND CRISPR POOLED SCREENS
    Publication number: 20190194653
    Abstract: An expression construct is disclosed which comprises: (i) a DNA sequence which encodes at least one guide RNA (gRNA) operatively linked to a transcriptional regulatory sequence so as to allow expression of the gRNA in a target cell; (ii) a barcode sequence for identification of the at least one gRNA operatively linked to a transcriptional regulatory sequence so as to allow expression of the barcode sequence in the target cell.
    Type: Application
    Filed: September 14, 2017
    Publication date: June 27, 2019
    Applicant: Yeda Research and Development Co. Ltd.
    Inventors: Ido AMIT, Diego JAITIN, David LARA-ASTIASO, Assaf WEINER, Ido YOFE
  • METHODS AND KITS FOR ANALYZING DNA BINDING MOIETIES ATTACHED TO DNA
    Publication number: 20170037453
    Abstract: A method of analyzing DNA which, in a cell, is bound to a DNA binding moiety, the method comprising: (a) obtaining at least two samples of complexes of DNA bound to a DNA binding moiety; (b) isolating the complexes on a solid support; (c) labeling the DNA of the complexes, wherein the labeling distinguishes between the complexes of the first of the at least two samples and the complexes of the second of the at least two samples; (d) pooling the at least two samples of complexes; and (e) isolating the complexes using an agent which specifically binds to the DNA binding moiety; and (f) analyzing the DNA of the complexes.
    Type: Application
    Filed: April 16, 2015
    Publication date: February 9, 2017
    Inventors: Ido AMIT, David LARA - ASTIASO, Meital GURY