Abstract: A method for generating an image of a region of interest in a biological sample comprising the steps of: generating a first image including the region of interest of the biological sample having undergone a first protocol but not a second protocol; and generating a second image including the region of interest of the biological sample after having undergone a second protocol; wherein the region of interest is smaller than said sample. Also provided is a method of analyzing a biological sample, comprising providing an image of the biological sample according to the method for generating an image of a region of interest in a biological sample, and analyzing the biological sample from the image. Further provided are system and kit that comprise the means for executing the novel methods.

Abstract: Embodiments provide a user interface including an image panel for displaying a field of view corresponding to an image, or a portion of an image, of a biological specimen, and a biomarker panel for displaying thumbnail images for simultaneously viewing different aspects of a region within the field of view. Upon selection or update of the selected region of the field of view, the thumbnail images may be accordingly updated automatically to display the newly selected region. The image panel may include an interest region selection component for delineating a region within the field of view. The interest region selection component may be overlaid over a portion of the field of view displayed in the image panel, and may be used by a user to select or update the region within the field of view displayed in the biomarker panel. Images of interest may be saved to an electronic record.

Abstract: Embodiments provide a user interface including an image panel for displaying a field of view corresponding to an image, or a portion of an image, of a biological specimen, and a biomarker panel for displaying thumbnail images for simultaneously viewing different aspects of a region within the field of view. Upon selection or update of the selected region of the field of view, the thumbnail images may be accordingly updated automatically to display the newly selected region. The image panel may include an interest region selection component for delineating a region within the field of view. The interest region selection component may be overlaid over a portion of the field of view displayed in the image panel, and may be used by a user to select or update the region within the field of view displayed in the biomarker panel. Images of interest may be saved to an electronic record.

Abstract: A method of analyzing a biological sample suspected of having classic Hodgkin lymphoma, comprising (1) detecting, in a single sample, for the expression of at least two biomarkers selected from CD30, CD15, CD20, CD45, CD3, Pax-5, CD79A, BOB1 and OCT-2; and (b) analyzing the sample based on the presence, absence and/or expression level of the at least two biomarkers. Also provided is a method wherein all the nine biomarkers are analyzed on a single sample. Further provided are method for diagnosing classical Hodgkin lymphoma, as well as system and kit that comprise the means for executing the novel methods.

Abstract: A method for providing a composite image of a single biological sample from a patient suspected of having classical Hodgkin lymphoma, comprising the steps of generating a first image of the biological sample including the presence, absence and/or expression level of a first biomarker, generating a second image of the biological sample including the presence, absence and/or expression level of a second biomarker, and generating a composite image that provides the relative location or expression of both biomarkers. Also provided is a method of analyzing a biological sample, comprising providing a composite image of the biological sample according to the method for providing a composite image, and analyzing the expression of the biomarkers of interest from the composite image. Further provided are method for diagnosing classical Hodgkin lymphoma, as well as system and kit that comprise the means for executing the novel methods.

Abstract: A method for providing a composite image of a single biological sample, comprising the steps of generating a first image of the biological sample, generating a second image of the biological sample, and generating a composite image that provides the relative location of both the target protein and the target nucleic acid. Also provided is a method of analyzing a biological sample, comprising providing a composite image of the biological sample according to the method for providing a composite image, and analyzing the expression of the protein and the nucleic acid sequences of interest from the composite image. Further provided are system and kit that comprise the means for executing the novel methods.

Abstract: A method for generating an image of a region of interest in a biological sample comprising the steps of: generating a first image including the region of interest of the biological sample having undergone a first protocol but not a second protocol; and generating a second image including the region of interest of the biological sample after having undergone a second protocol; wherein the region of interest is smaller than said sample. Also provided is a method of analyzing a biological sample, comprising providing an image of the biological sample according to the method for generating an image of a region of interest in a biological sample, and analyzing the biological sample from the image. Further provided are system and kit that comprise the means for executing the novel methods.

Abstract: In an imaging device having an objective and a stage for holding a sample to be imaged, a method for autofocusing is presented. The method includes determining a measured focus value corresponding to at least a first of a plurality of logical image segments. Further, the method includes imaging the first logical image segment using the measured focus value. The method also includes determining a predicted focus value for a second of the plurality of logical image segments using the measured focus value and a stored focus variation parameter. In addition, the method includes imaging the second logical image segment using the predicted focus value.

Abstract: A method for imaging is presented. The method includes acquiring a plurality of images corresponding to at least one field of view using an imaging device having an objective and a stage for holding a sample to be imaged. Furthermore, the method includes determining a figure of merit corresponding to each pixel in each of the plurality of acquired images, wherein the figure of merit comprises a discrete approximation to a gradient vector. The method also includes synthesizing a composite image based upon the determined figures of merit.

Abstract: A method for imaging is presented. The method includes acquiring a plurality of images corresponding to at least one field of view at a plurality of sample distances. Furthermore, the method includes determining a figure of merit corresponding to each pixel in each of the plurality of acquired images. The method also includes for each pixel in each of the plurality of acquired images identifying an image in the plurality of images that yields a best figure of merit for that pixel. Moreover, the method includes generating an array for each image in the plurality of images. In addition, the method includes populating the arrays based upon the determined best figures of merit to generate a set of populated arrays. Also, the method includes processing each populated array in the set of populated arrays using a bit mask to generate bit masked filtered arrays. Additionally, the method includes selecting pixels from each image in the plurality of images based upon the bit masked filtered arrays.

Abstract: A method for imaging is presented. The method includes acquiring a plurality of images corresponding to overlapping fields of view at a plurality of sample distances using an imaging device having an objective and a stage for holding a sample to be imaged. Moreover, the method includes determining a figure of merit corresponding to each pixel in each of the plurality of acquired images. The method also includes synthesizing a composite image based upon the determined figures of merit.

Abstract: A method for imaging is presented. The method includes acquiring a plurality of images corresponding to at least one field of view using an imaging device having an objective and a stage for holding a sample to be imaged. Furthermore, the method includes determining a figure of merit corresponding to each pixel in each of the plurality of acquired images, wherein the figure of merit comprises a discrete approximation to a gradient vector. The method also includes synthesizing a composite image based upon the determined figures of merit.

Abstract: In an imaging device having an objective and a stage for holding a sample to be imaged, a method for autofocusing is presented. The method includes determining a measured focus value corresponding to at least a first of a plurality of logical image segments. Further, the method includes imaging the first logical image segment using the measured focus value. The method also includes determining a predicted focus value for a second of the plurality of logical image segments using the measured focus value and a stored focus variation parameter. In addition, the method includes imaging the second logical image segment using the predicted focus value.

Abstract: An Integration Controller integrates broadcast automation systems and thereby provides a single command and control center for monitoring production of the audio and video stream. An event-based schedule, or a “playlist,” specifies the segments to be played along with the necessary timing and hardware information. Each event in the schedule commands the playback or record of a video segment, generation of a special effect, transfer of a video segment from one device to another or closure of one or more video switches. Events may be launched automatically at a specified time, or manually by an operator. An Integration Controller (IC) communicates with an Event Scheduling System via a Scheduling System Interface. The Scheduling System Interface communicates with the Integration Controller via the IC server. The IC server utilizes various modules to communicate with Device Drivers, a Log Manager and one or more User Interfaces.