Abstract: Described herein are platforms and consumables for performing gene/siRNA/protein/peptide delivery screens in high throughput, including an automation-compatible transfection methodology that can be used to gain entry of oligonucleotides, proteins, peptides and other non-permeable molecules (e.g., some small organic compounds) into cells. Electrical stimulation is provided to cells, e.g., neurons or myocytes, in culture through application of pulsed fields directly to the cells in culture. The plate design is used in conjunction with a custom-designed circuit that allows the user to select pulse type, duration, etc.

Abstract: The present invention provides binding assays, referred to here as fluorescence proximity assays or FPA. The inventions detect binding of target molecules in a sample to a molecular probe or probes that specifically bind or hybridize to those molecules. In particular, the molecular probes are immobilized to a bead or particle, such as colloidal gold, the reflects fluorescent energy from a fluorophore. The derivatized beads are contacted to a sample of fluorescently labeled target molecules, and binding of the target is indicated by an increase in the fluorescent signal. Kits are also provided that contain materials and reagents to performing a fluorescence proximity assay.