Abstract: A computer-implemented method for collaborative knowledge management, the method comprises the steps of: (a) providing an interface for users to create or edit pages of a collaborative knowledge base, to add semantic annotations to the pages, and to define dynamic data on the pages; wherein ontological statements are created for the semantic annotations to form part of an ontology; and the dynamic data is defined using a query against the ontology and a mathematical operation on a result of the query; and (h) when a page with dynamic data is accessed, dynamically performing the query and the mathematical operation, and presenting the page with a result of the query or a result of the mathematical operation, or both, in graphical form.

Abstract: Loblolly pine (Pinus taeda L.) is the most important commercial tree species in the USA harvested for pulp and solid wood products. Increasing the efficiency of chemical pulping may be achieved through the manipulation of genes involved in the lignin biosynthetic pathway. A null allele of cinnamyl alcohol dehydrogenase (CAD) has been discovered in the loblolly pine clone 7-56 which displays altered lignin composition. During identification of single nucleotide polymorphisms (SNPs) in the cad gene, a two-base pair adenosine insertion located in exon five and unique to clone 7-56 was discovered. The sequence mutation causes a frame-shift predicted to result in premature termination of the protein. For routine detection of the mutation, a diagnostic assay was developed utilising Template-directed Dye-terminator Incorporation and Fluorescence Polarization detection (FP-TDI).