Abstract: The disclosure provides methods and compositions for treating blood diseases/disorders, such as sickle cell disease, hemochromatosis, hemophilia, and beta-thalassemia. For example the disclosure provides therapeutic guide RNAs that target the promotor of HBG1/2 to generate point mutations that increase expression of fetal hemoglobin. As another example, the disclosure provides therapeutic guide RNAs that target mutations in HBB, Factor VIII, and HFE to treat sickle cell disease, beta-thalassemia, hemophilia and hemochromatosis. The disclosure also provides fusion proteins comprising a Cas9 (e.g., a Cas9 nickase) domain and adenosine deaminases that deaminate adenosine in DNA. In some embodiments, the fusion proteins are in complex with nucleic acids, such as guide RNAs (gRNAs), which target the fusion proteins to a DNA sequence (e.g., an HBG1 or HBG2 protmoter sequence, or an HFE, GBB, or F8 gene sequence).

Abstract: The present disclosure is directed to an electronic deadbolt control system including a deadbolt configured to extend or retract between a locked position and an unlocked position, respectively. An output shaft connected between a final gear and the deadbolt is configured to transmit an actuation force to the deadbolt from an electric motor. A first magnet and a second magnet are associated with the final gear to define a home position for either a left hand deadbolt or a right hand deadbolt. A cam is positioned on the output shaft to engage with a switch such that, in combination with a threshold current motor output, the control system determines whether the deadbolt is in an extended position or a retracted position. A thumb-turn shaft is disengaged from the final gear in the home position to permit manual actuation of a thumb-turn.

Abstract: The present disclosure is directed to an electronic deadbolt control system including a deadbolt configured to extend or retract between a locked position and an unlocked position, respectively. An output shaft connected between a final gear and the deadbolt is configured to transmit an actuation force to the deadbolt from an electric motor. A first magnet and a second magnet are associated with the final gear to define a home position for either a left hand deadbolt or a right hand deadbolt. A cam is positioned on the output shaft to engage with a switch such that, in combination with a threshold current motor output, the control system determines whether the deadbolt is in an extended position or a retracted position. A thumb-turn shaft is disengaged from the final gear in the home position to permit manual actuation of a thumb-turn.

Abstract: The present disclosure is directed to an electronic deadbolt control system including a deadbolt configured to extend or retract between a locked position and an unlocked position, respectively. An output shaft connected between a final gear and the deadbolt is configured to transmit an actuation force to the deadbolt from an electric motor. A first magnet and a second magnet are associated with the final gear to define a home position for either a left hand deadbolt or a right hand deadbolt. A cam is positioned on the output shaft to engage with a switch such that, in combination with a threshold current motor output, the control system determines whether the deadbolt is in an extended position or a retracted position. A thumb-turn shaft is disengaged from the final gear in the home position to permit manual actuation of a thumb-turn.

Abstract: The present disclosure is directed to an electronic deadbolt control system including a deadbolt configured to extend or retract between a locked position and an unlocked position, respectively. An output shaft connected between a final gear and the deadbolt is configured to transmit an actuation force to the deadbolt from an electric motor. A first magnet and a second magnet are associated with the final gear to define a home position for either a left hand deadbolt or a right hand deadbolt. A cam is positioned on the output shaft to engage with a switch such that, in combination with a threshold current motor output, the control system determines whether the deadbolt is in an extended position or a retracted position. A thumb-turn shaft is disengaged from the final gear in the home position to permit manual actuation of a thumb-turn.

Abstract: The present disclosure is directed to an electronic deadbolt control system including a deadbolt configured to extend or retract between a locked position and an unlocked position, respectively. An output shaft connected between a final gear and the deadbolt is configured to transmit an actuation force to the deadbolt from an electric motor. A first magnet and a second magnet are associated with the final gear to define a home position for either a left hand deadbolt or a right hand deadbolt. A cam is positioned on the output shaft to engage with a switch such that, in combination with a threshold current motor output, the control system determines whether the deadbolt is in an extended position or a retracted position. A thumb-turn shaft is disengaged from the final gear in the home position to permit manual actuation of a thumb-turn.