Abstract: The present invention provides novel compositions comprising Epstein-Barr virus-specific oligonucleotides that are useful as primers to amplify particular regions of the genome during enzymatic nucleic acid amplification. The invention also provides a rapid, sensitive and specific method for the detection and quantitation of the virus which may be present in a clinical specimen, using the virus-specific primers and enzymatic nucleic acid amplification; hybridization of amplified target sequences, if present, with one or more Epstein-Barr virus-specific oligonucleotide probes which are labeled with a detectable moiety; and detection of the detectable moiety of labeled oligonucleotide probe hybridized to amplified target sequences of Epstein-Barr virus DNA.

Abstract: The present invention provides novel compositions comprising Epstein-Barr virus-specific oligonucleotides that are useful as primers to amplify particular regions of the genome during enzymatic nucleic acid amplification. The invention also provides a rapid, sensitive and specific method for the detection and quantitation of the virus which may be present in a clinical specimen, using the virus-specific primers and enzymatic nucleic acid amplification; hybridization of amplified target sequences, if present, with one or more Epstein-Barr virus-specific oligonucleotide probes which are labeled with a detectable moiety; and detection of the detectable moiety of labeled oligonucleotide probe hybridized to amplified target sequences of Epstein-Barr virus DNA.

Abstract: A continuous cell line of megakaryocytic origin is disclosed. This novel continuous cell line has been established in athymic nude mice. The cells of the established cell line monophenotypic for megakaryocytes is characterized as having multilobed nuclei and granular cytoplasm and being reactive for Factor VIII antigen, glycoprotein IIB-IIIa complex antigen and platelet peroxidase. The established cell line of this invention generates in isolatable quantities platelet-like factor IV, FGF-like, .beta.-thromboglobulin-like and TGF-.beta.-like growth factors. Methods for cultivating the established cell line, in a nude animal and isolating and purifying the proteins are also disclosed.