Abstract: Disclosed herein are methods and compositions for the identification of viability enhancing cell features and substrate features as they relate to post-cryopreservation survival of substrate seeded cells. Embodiments of the present invention further involve identification of cell features to manufacture a supernatant that is useful for cell culturing and treatment of various diseases.

Abstract: Disclosed herein are methods and compositions for the identification of viability enhancing cell features and substrate features as they relate to post-cryopreservation survival of substrate seeded cells. Embodiments of the present invention further involve identification of cell features to manufacture a supernatant that is useful for cell culturing and treatment of various diseases.

Abstract: Disclosed herein are methods and compositions for the cryopreservation of stem cells, such as stem-cell derived retinal pigment epithelial cells, that have been seeded onto and cultured on a substrate, such as a polymeric substrate. Such cryopreserved stem cells are useful for cell therapies, such as treatment of ocular damage or disease.

Abstract: Disclosed herein are substrates for cell delivery to target tissues requiring treatment for various diseases that induce cell death, damage or loss of function. The substrates are configured to provide seeded cells, including stem cells, with a structural support that allows interconnection with and transmission of biological signals between the cells and the target tissue.

Abstract: Disclosed herein are methods and compositions for the identification of viability enhancing cell features and substrate features as they relate to post-cryopreservation survival of substrate seeded cells. Embodiments of the present invention further involve identification of cell features to manufacture a supernatant that is useful for cell culturing and treatment of various diseases.

Abstract: Disclosed herein are methods and compositions for the cryopreservation of stem cells, such as stem-cell derived retinal pigment epithelial cells, that have been seeded onto and cultured on a substrate, such as a polymeric substrate. Such cryopreserved stem cells are useful for cell therapies, such as treatment of ocular damage or disease.

Abstract: Disclosed herein are substrates for cell delivery to target tissues requiring treatment for various diseases that induce cell death, damage or loss of function. The substrates are configured to provide seeded cells, including stem cells, with a structural support that allows interconnection with and transmission of biological signals between the cells and the target tissue.

Abstract: The production of high quality retinal pigmented epithelium (RPE) cells is necessary for research and potential therapeutic uses. Especially desirable are methods for the production of RPE cells using xeno-free culture conditions. Disclosed herein are novel methods for the production of RPE cells from pluripotent cells with high yields, including xeno-free production methods. Also provided are methods of efficiently isolating RPE cells from cultures containing heterogeneous cell types, allowing for substantially pure RPE cell cultures to be established. Additionally, novel methods for the cryopreservation of RPE cells are provided.

Abstract: Disclosed herein are substrates for cell delivery to target tissues requiring treatment for various diseases that induce cell death, damage or loss of function. The substrates are configured to provide seeded cells, including stem cells, with a structural support that allows interconnection with and transmission of biological signals between the cells and the target tissue.

Abstract: The present invention relates to methods for inhibiting or preventing ocular processes associated with CTGF. Methods and agents for use in treating or preventing ocular disorders, methods for diagnosing ocular disorders and related kits, and methods for screening for agents for use in the present methods are also provided.

Abstract: A wheel and final drive assembly for a work machine, such as a mining truck, having a driven rotatable wheel having inboard and outboard rims mounted thereto. The final drive assembly including a first and a second reduction gear assembly. A ring gear adapter having an inboard end, an outboard end, and an inner surface, a plurality of teeth disposed about the inner surface and interacting with a plurality of planetary gears of the first and second gear reduction assemblies and the inboard end of the ring gear adapter removably coupled to the outboard flange of the wheel.

Abstract: A wheel and final drive assembly for a work machine, such as a mining truck, having a driven rotatable wheel having inboard and outboard rims mounted thereto. The final drive assembly including a first and a second reduction gear assembly. A carrier output adapter having an inboard end, an outboard end, a cover attached to the outboard end and a mounting surface. The carrier adapter being attached at the inboard mounting surface to a outboard rim mounting ring and at the cover to a carrier assembly of the second reduction gear assembly.

Abstract: A brake assembly for braking a wheel having a brake anchor, a brake anchor first annular portion, a brake anchor second annular portion, and a plurality of brake anchor splines on the brake anchor first annular portion.

Abstract: A wheel and final drive assembly for a work machine, such as a mining truck, having a driven rotatable wheel having inboard and outboard rims mounted thereto. The final drive assembly including a first and a second reduction gear assembly. A ring gear adapter having an inboard end, an outboard end, and an inner surface, a plurality of teeth disposed about said inner surface and interacting with a plurality of planetary gears of the first and second gear reduction assemblies and said inboard end of said ring gear adapter removably coupled to said outboard flange of said wheel.

Abstract: A wheel and final drive assembly for a work machine, such as a mining truck, having a driven rotatable wheel having inboard and outboard rims mounted thereto. The final drive assembly including a first and a second reduction gear assembly. A carrier output adapter having an inboard end, an outboard end, a cover attached to the outboard end and a mounting surface. The carrier adapter being attached at the inboard mounting surface to a outboard rim mounting ring and at the cover to a carrier assembly of the second reduction gear assembly.

Abstract: A brake assembly for braking a wheel having a brake anchor, a brake anchor first annular portion, a brake anchor second annular portion, and a plurality of brake anchor splines on the brake anchor first annular portion.

Abstract: A mechanical differential assembly having an increased bearing load zone capacity. The mechanical gear assembly maintains a proper load capacity of rollers of the bearings which support a differential gear assembly. The mechanical differential assembly thus maintains alignment of the inner and outer races of the bearings regardless of an axial force placed on the bearings, any loads placed on the differential gear assembly or the like during driving condition. The mechanical differential assembly includes a differential gear assembly supported on a first end by a cylindrical bearing and supported on the second end by a pair of tapered bearings.

Abstract: This invention relates to a method and apparatus for maintaining equal air pressure in a pair of tires on a work machine. The invention provides a valve body with a pair of piston chambers. A piston is reciprocatably positioned within each piston chamber, the pistons are biased toward a first closed end of the piston chambers. The closed end of each piston chamber is connected by a passageway to one of the tires. Each piston chamber is additionally fluidly connected to the other piston chamber. When the air pressure in each tire is above a predetermined amount the pistons are moved away from the closed end of the piston chamber and airflow is permitted between the piston chambers thereby balancing the tire pressures. If the pressure in either tire is below the predetermined minimum, the piston in that respective piston chamber moves toward the first end to block, airflow between the piston chambers.

Abstract: This invention relates to a method and apparatus for maintaining equal air pressure in a pair of tires on a work machine. The invention provides a valve body with a pair of piston chambers. A piston is reciprocatably positioned within each piston chamber, the pistons are biased toward a first closed end of the piston chambers. The closed end of each piston chamber is connected by a passageway to one of the tires. Each piston chamber is additionally fluidly connected to the other piston chamber. When the air pressure in each tire is above a predetermined amount the pistons are moved away from the closed end of the piston chamber and airflow is permitted between the piston chambers thereby balancing the tire pressures. If the pressure in either tire is below the predetermined minimum, the piston in that respective piston chamber moves toward the first end to block, airflow between the piston chambers.

Abstract: An apparatus for controlling temperature of fluid in a rear axle assembly includes a rear axle housing having an internal component chamber. The internal component chamber has a rear axle sump located therein. The apparatus also includes a temperature control operational pressure source coupled to the internal component chamber. The apparatus further includes a diverter valve coupled to the temperature control operational pressure source. The apparatus includes a heating fluid return line interposed between the diverter valve and the rear axle sump, and includes a cooling fluid return line interposed between the diverter valve and the rear axle housing. The temperature control operational pressure source is selectively operable between (i) a first rate mode in which it advances fluid from the internal component chamber at a first flow rate, and (ii) a second rate mode in which it advances fluid from the internal component chamber at a second flow rate.