Abstract: Novel Bacillus lysin proteins have been identified and characterized. Methods to detect the presence or absence of bacteria in a sample, methods for lysing bacteria, and methods for decontaminating or disinfecting areas contaminated with bacteria that utilize these lysin proteins have also been developed.

Abstract: Novel Bacillus lysin proteins have been identified and characterized. Methods to detect the presence or absence of bacteria in a sample, methods for lysing bacteria, and methods for decontaminating or disinfecting areas contaminated with bacteria that utilize these lysin proteins have also been developed.

Abstract: Disclosed herein are the identification, cloning, and optimizing the lytic activity of one or more novel Bacillus lysin proteins, a method of selecting a lysin agent for use in molecular diagnostic testing that includes analyzing genome databases for Bacillus anthracis and near neighbors, selecting candidate genes encoding potential lytic enzymes based on conserved amino acid motifs as determined in peptidoglycan hydrolases, cloning the candidate genes in expression vector, isolating proteins thereof, and testing for lytic activity against Bacillus anthracis, and selecting an optimum gene of the candidate genes for optimizing lysis conditions.

Abstract: Disclosed herein are the identification, cloning, and optimizing the lytic activity of one or more novel Bacillus lysin proteins, a method of selecting a lysin agent for use in molecular diagnostic testing that includes analyzing genome databases for Bacillus anthracis and near neighbors, selecting candidate genes encoding potential lytic enzymes based on conserved amino acid motifs as determined in peptidoglycan hydrolases, cloning the candidate genes in expression vector, isolating proteins thereof, and testing for lytic activity against Bacillus anthracis, and selecting an optimum gene of the candidate genes for optimizing lysis conditions.

Abstract: The self-locking chock system has a plurality of pawl cams grouped in a sequential alignment along a support member that is substantially parallel with a leg rack of a jack-up rig unit. Each of the cams is pivoted about a pivot axis that is on the common alignment axis of the support member, and is forced to ride upon the leg rack by a center of gravity located outwardly from the alignment axis on the side opposite that which rides on the leg rack. The cam profile is adapted to ride along the leg rack profile when the relative motion between the leg rack and the support member is in a first direction. When the relative motion is in a direction opposite to the first direction, the cam profile complements the leg-rack profile and under the weighted urging of the cam, the cam profile engages the leg-rack profile and enters into a positive, self-locking engagement that restrains further relative motion between the leg rack and the support member at least in the opposite direction.