Abstract: An automatic oil dispenser (10) for mounting onto an objective (14) in an inverted microscope system, the dispenser comprising: an outer annulus (52) configured to contact the objective to form a cavity; an inlet port (20) below the outer annulus (52) for inputting oil into the outer annulus (52); a pump for driving oil upwards through and out of the dispenser; an inner annulus (56) which tapers to an annular outlet (36); and a channel (53) connecting the two annuli such that oil fills the outer annulus (56), then the inner annulus (52) and then erupts through the outlet (36) onto an objective lens (26).

Abstract: A chip-based optical detection system for detecting a target component in a liquid sample is provided. The system comprises a fluid pathway disposed in an interior volume of the chip; an inlet fluidically connecting an external surface of the chip to the fluid pathway; an optical element at least partially defining the fluid pathway; and a flow controller configured to control a flow of the liquid sample through the fluid pathway. One or more capture components are immobilised on the optical element, one or more detection reagents are provided in a proximity of the capture components, and the flow controller is configured to control the flow of the liquid sample along the fluid pathway such that the detection reagents are brought into contact with the capture components via diffusion.

Abstract: A sample management module for collecting a fluid sample is provided. The module comprising; a sample collection device comprising a sample collection location; a decoupling zone configured to receive sample from the collection device, an overflow reservoir in fluid communication with the decoupling zone to accommodate any sample that does not fit into the decoupling zone; wherein the overflow reservoir is configured to ensure pressure within the module can be managed. A cartridge comprising a sample management module is also provided.

Abstract: A substrate for facilitating the measurement, using Total Internal Reflection microscopy, of the status of an assay; a first surface including a test site at which one or more reagents for the assay are immobilised; a second surface substantially parallel to the first surface; a third surface joining the first and second surfaces; a fourth surface joining the first and second surfaces; wherein three characteristics of the surfaces are manipulated to ensure that light launched through the third surface impinges on the centre of the first surface solely at the test site and then undergoes Total Internal Reflection; wherein the three characteristics are: the length of the first and second surfaces between the third and fourth surfaces; the length of the third and fourth surfaces; the angle subtended between the first and third surfaces.

Abstract: A device configured to detect signals indicative of a binding event in an assay of interest is provided. The device comprising a beam manipulator configured to modify an incident beam to form a beam with a sharp-edged intensity cut-off; a test site having immobilised thereon at least one component of the assay of interest; wherein the device is configured so that the sharp-edged intensity cut-off beam produced by the beam manipulator is incident, in use, on the test site at such an angle to facilitate Total Internal Reflection (TIR); wherein the device is configured so that the sharp-edged intensity cut-off beam produced by the beam manipulator substantially conforms to the shape and size of the test site; a detector configured to receive a signal indicative of a binding event in the assay of interest; wherein the test site is located within a microfluidic channel.

Abstract: A sample management module for receiving a fluid sample for an immunoassay is provided. The module comprises: a sample reception device for receiving a sample; a detection reagent; a controller configured to initiate a reaction timer on the basis of an actuation event when the sample and detection reagent are brought into contact.

Abstract: A storage and incubation device is provided. The device is configured to accommodate a plurality of assay chips, each assay chip including a unique identifier. The device comprises: a plurality of berths each sized to accommodate an assay chip; a communication module configured to receive information about the identifier of each assay chip; a clock timer configured to monitor the timing of the assay within each assay chip; a completion module to manage each assay chip when the assay is complete.

Abstract: A combined sample collection and filtration device is provided for collecting and filtering a fluid sample. The device comprises: a sample collection location; a filter module for removing particulate matter from the fluid sample; the filter module having bubble point and a burst pressure; and one or more voids in communication with the sample collection location for accommodating sample during pressurisation such that the pressure within the device remains below the bubble point and the burst pressure of the filter module.

Abstract: An apparatus for creating and correcting a two dimensional intensity map of one or more assay spots in a detection zone is provided. The apparatus comprising, a locator for affirming the location of the detection zone; a total internal reflection excitation device comprising a light source for illuminating the one or more assay spots in the detection zone; a detector configured to receive light that is emitted, reflected or scattered from the one or more assay spots and to create a two dimensional intensity map of the one or more assay spots comprising a two dimensional array of quantitative pixel values; and a processor configured to correct the intensity map to remove noise through analysis of corresponding pixel values from an earlier intensity map; wherein the analysis includes curve fitting.

Abstract: An apparatus (1) for detecting the presence and/or the quantity of a target component in a biological fluid in an integrated assay cartridge (52) of predetermined configuration, the assay cartridge comprising a capture component (22) at a predetermined location in the assay cartridge, the apparatus comprising: a detector (12)for detecting the amount of light scattered, transmitted or emitted by the sample to provide an indication of the presence and/or the quantity of the target component within the sample; three location positions (30), the three positions defining a location along the optical path of the detector on which to locate the cartridge of a predetermined size; wherein location positions are configured such that the capture component of the assay cartridge is located, in use, at the focal plane of the detector. Measures to ensure quality control may also be provided.

Abstract: An assay cartridge for detecting a target component in a fluid is provided. The assay cartridge including an optical element comprising: a light pathway comprising an input surface, reflective surface and output surface configured to enable light to enter, reflect and create an evanescent field in the vicinity of the reflective surface and exit the element; a plurality of capture components deposited on the reflective surface in the vicinity of the evanescent field; and a transmission surface configured to enable emissions from the evanescent field to exit the element; wherein the assay cartridge is a single use cartridge.

Abstract: A sample management module for collecting a fluid sample is provided. The module comprising; a sample collection device comprising a sample collection location; a decoupling zone configured to receive sample from the collection device, an overflow reservoir in fluid communication with the decoupling zone to accommodate any sample that does not fit into the decoupling zone; wherein the overflow reservoir is configured to ensure pressure within the module can be managed. A cartridge comprising a sample management module is also provided.

Abstract: An assay cartridge for detecting a target component in a liquid sample is provided. The cartridge comprises: a sample collection unit configured to introduce the liquid sample into the cartridge; a fluid pathway commencing at its proximal end at the sample collection unit and extending distally through the cartridge including: one or more capture components immobilised within the fluid pathway; one or more detection reagents provided within the diffusion distance of the capture components.

Abstract: An assay cartridge for detecting a target component in a liquid sample is provided. The cartridge comprises: a sample collection unit configured to introduce the liquid sample into the cartridge; a fluid pathway commencing at its proximal end at the sample collection unit and extending distally through the cartridge including: one or more capture components immobilised within the fluid pathway; one or more detection reagents provided proximally of or level with the capture components each contained within a liquid droplet.

Abstract: A method of determining an analyte parameter is described for an analyte in a detection region having at least one analyte binding zone. The method includes detecting presence of an analyte element bound at the binding zone, incrementing or decrementing a count and preventing recount of the element to obtain absolute quantification.

Abstract: The present invention provides an improved method of analysing and obtaining reliable data about the plasma membrane of single cells, using a microfluidic cell analyser. The microfluidic cell analyser of the invention comprises a single cell trap, a manipulator arranged to manipulate the outer surface of a cell in the trap, a detection zone in communication with the single cell trap and a detector.

Abstract: A high energy photon source for generating EUV radiation comprises a nozzle emitting a supersonic stream of source material, a laser or electrical/magnetic pre-ionization mechanism and a laser or electrical/magnetic excitation mechanism and a skimmer plate between them providing a collimated high density beam of source material for excitation.

Abstract: A laser is disclosed which includes a gain medium, a switch element, and a pulse controller. In one embodiment laser light of differing polarizations pass along respective paths and a pulsed laser output is generated via an electro-optical element. In another embodiment light of differing polarizations passes in differing directions through a cyclical path. The invention can make use of a prism-shaped polarizer having a polarization selection face and two further faces. Yet further initial pulses can be controlled to reduce energy, for example by progressively increasing the period or amplitude of successive pulses. As a result an efficient and high power laser apparatus is realized.

Abstract: A laser is disclosed which includes a gain medium, a switch element, and a pulse controller. In one embodiment laser light of differing polarizations pass along respective paths and a pulsed laser output is generated via an electro-optical element. In another embodiment light of differing polarizations passes in differing directions through a cyclical path. The invention can make use of a prism-shaped polarizer having a polarization selection face and two further faces. Yet further initial pulses can be controlled to reduce energy, for example by progressively increasing the period or amplitude of successive pulses. As a result an efficient and high power laser apparatus is realized.

Abstract: An extreme ultraviolet radiation generator is disclosed in which Xenon gas is continuously ejected from a high pressure nozzle into a low pressure chamber to generate Xenon atom clusters which are irradiated with a high repetition rate pulsed laser to form a plasma and yield quasi-continuous EUV generation. The nozzle has a beveled outer rim to enable the focus point of the laser light to be brought close to the nozzle. The nozzle is cooled to a temperature at which background Xenon gas condenses onto the nozzle forming a protective layer. A gas compressor serves to recirculate the Xenon gas and batch purification triggered by a mass spectrometer monitoring gas purity may be periodically applied.