Abstract: According to some embodiments, a method comprises obtaining data in multiple modalities, including data in an image modality and data in a biomarker modality; obtaining one or more trained machine-learning models; generating a first multi-modality group from the data in multiple modalities, wherein the first multi-modality group includes data in at least the image modality and in the biomarker modality; generating a group of intermediate data, wherein generating the group of intermediate data includes inputting the first multi-modality group into a machine-learning model that has been trained to extract features from input multi-modality data and output the features as the intermediate data; and generating a first classification result based on at least the group of intermediate data, wherein generating the first classification result includes inputting the intermediate data into a machine-learning model that has been trained to output the classification result based on the group of intermediate data.

Abstract: The present invention relates to systems and methods for detecting analyte molecules or particles in a fluid sample and in some cases, determining a measure of the concentration of the molecules or particles in the fluid sample. Methods of the present invention may comprise immobilizing a plurality of analyte molecules or particles with respect to a plurality of capture objects. At least a portion of the plurality of capture objects may be spatially separated into a plurality of locations. A measure of the concentration of analyte molecules in a fluid sample may be determined, at least in part, on the number of reaction vessels comprising an analyte molecule immobilized with respect to a capture object. In some cases, the assay may additionally comprise steps including binding ligands, precursor labeling agents, and/or enzymatic components.

Abstract: The present invention relates to novel biomarkers and combinations thereof for cell type-specific and/or organ-specific extracellular vesicles, in particular, brain-specific and/or neuron-specific extracellular vesicles. The present invention also provides methods for isolation and/or enrichment of cell type-specific and/or organ-specific extracellular vesicles, methods for identification of extracellular vesicles derived from a cell, and methods for diagnosing or prognosing a disorder, e.g., a neurodegenerative disorder, using the cell type specific and/or organ-specific extracellular vesicles. Compositions in the form of kits of reagents for detecting the cell type-specific and/or organ-specific extracellular vesicles are also provided.

Abstract: A medical information processing apparatus according to an embodiment includes processing circuitry configured to acquire a medical image obtained by imaging a subject, acquire a value of a biomarker contained in a sample collected from the subject, determine a region of interest on the acquired medical image, analyze the image of the determined region of interest and calculate a property of a drawn object, select a value of a biomarker associated with the calculated property of the drawn object, and determine consistency between the selected value of the biomarker and the calculated property of the drawn object.

Abstract: A medical information processing apparatus according to an embodiment includes a first analysis unit, a second analysis unit, a determination unit, and a third analysis unit. The first analysis unit executes a first analysis based on a medical image of a subject. The second analysis unit executes a second analysis based on a specimen examination result of the subject. The determination unit determines a matching degree between the result of the first analysis by the first analysis unit and the result of the second analysis by the second analysis unit. The third analysis unit executes a third analysis based on the medical image and the specimen examination result according to analysis conditions based on the determination result of the matching degree by the determination unit, and determines diagnosis support information based on the medical image and the specimen examination result.

Abstract: The present invention relates to systems and methods for detecting analyte molecules or particles in a fluid sample and in some cases, determining a measure of the concentration of the molecules or particles in the fluid sample. Methods of the present invention may comprise immobilizing a plurality of analyte molecules or particles with respect to a plurality of capture objects. At least a portion of the plurality of capture objects may be spatially separated into a plurality of locations. A measure of the concentration of analyte molecules in a fluid sample may be determined, at least in part, on the number of reaction vessels comprising an analyte molecule immobilized with respect to a capture object. In some cases, the assay may additionally comprise steps including binding ligands, precursor labeling agents, and/or enzymatic components.

Abstract: The invention provides methods and compositions for distinguishing breast cancer.

Abstract: The present invention relates to systems and methods for detecting analyte molecules or particles in a fluid sample and in some cases, determining a measure of the concentration of the molecules or particles in the fluid sample. Methods of the present invention may comprise immobilizing a plurality of analyte molecules or particles with respect to a plurality of capture objects. At least a portion of the plurality of capture objects may be spatially separated into a plurality of locations. A measure of the concentration of analyte molecules in a fluid sample may be determined, at least in part, on the number of reaction vessels comprising an analyte molecule immobilized with respect to a capture object. In some cases, the assay may additionally comprise steps including binding ligands, precursor labeling agents, and/or enzymatic components.

Abstract: The invention provides ultrasensitive methods for detection and quantification of target analytes in samples. The methods can be multiplexed to allow simultaneous detection and quantification of multiple target analytes. The methods can achieve an attomolar limit of detection. The invention also provides related compositions and kits.

Abstract: Described herein are systems and methods for extending the dynamic range of assay methods and systems used for determining the concentration of analyte molecules or particles in a fluid sample. In some embodiments, a method comprises spatially segregating a plurality of analyte molecules in a fluid sample into a plurality of locations. At least a portion of the locations may be addressed to determine the percentage of said locations containing at least one analyte molecule. Based at least in part on the percentage, a measure of the concentration of analyte molecules in the fluid sample may be determined using an analog, intensity-based detection/analysis method/system and/or a digital detection/analysis method/system. In some cases, the assay may comprise the use of a plurality of capture objects.

Abstract: Described herein are quantitative methods that can be used for diagnosis of neurodegenerative diseases, characterized partially by pathological protein aggregation and/or deposition, comprised of assaying in microwells, droplets, beads or some other support, the ability of a pathological protein in a biological sample to seed the conversion of substrate monomers into aggregated fibrils.

Abstract: Arrays of single molecules and methods of producing an array of single molecules are described. Arrays with defined volumes between 10 attoliters and 50 picoliters enable single molecule detection and quantitation.

Abstract: Methods and compositions for accurate blood biomarker panel-based detection of cancer, e.g., breast cancer, and sub-typing, e.g., using ultrasensitive immunoassays, e.g., digital ELISA.

Abstract: Arrays of single molecules and methods of producing an array of single molecules are described. Arrays with defined volumes between 10 attoliters and 50 picoliters enable single molecule detection and quantitation.

Abstract: Provided herein are assays that provide digital measurement methods to detect proteins and other biomolecules, e.g., at low- to mid-attomolar concentrations.

Abstract: The present disclosure provides novel methods to purify and quantify extracellular vesicles (EVs) in biological samples, e.g plasma or cerebrospinal fluid (CSF).

Abstract: The present invention relates to systems and methods for detecting analyte molecules or particles in a fluid sample and in some cases, determining a measure of the concentration of the molecules or particles in the fluid sample. Methods of the present invention may comprise immobilizing a plurality of analyte molecules or particles with respect to a plurality of capture objects. At least a portion of the plurality of capture objects may be spatially separated into a plurality of locations. A measure of the concentration of analyte molecules in a fluid sample may be determined, at least in part, on the number of reaction vessels comprising an analyte molecule immobilized with respect to a capture object. In some cases, the assay may additionally comprise steps including binding ligands, precursor labeling agents, and/or enzymatic components.

Abstract: Methods and compositions are disclosed herein to detect Mycobacterium tuberculosis antigens, e.g., lipoarabinomannan (LAM) and/or Ag85B (Rv1886c), in a sample (e.g., a human urine sample) for diagnosis of tuberculosis. By developing ultrasensitive assays, both antigens are detected, with quantifiable differences levels in TB patients vs. non-TB controls.

Abstract: The present invention generally relates to systems and methods for delivering and/or receiving a substance or substances such as blood, from subjects, e.g., from the skin and/or from other tissues of the body. In some cases, the device may contain a substance transfer component such as needles or microneedles, which can be inserted into the skin or another organ to deliver and/or receiving fluid or other substances from the subject. In some embodiments, the device may include an activator constructed and arranged to insert one or more substance transfer components into the skin or other organ. In certain cases, the device may also include a storage chamber for receiving a fluid received from the subject.

Abstract: Arrays of single molecules and methods of producing an array of single molecules are described. Arrays with defined volumes between 10 attoliters and 50 picoliters enable single molecule detection and quantitation.