Abstract: An apparatus and method for preparing frozen tissue specimens includes a base supporting a pair of rotary motion platforms and a center platform. The rotary motion platforms are movable from an open, side-by-side position to a closed, center platform-covering position. The rotary motion platform includes a plurality of cryogenic discs, each identified for receiving a plurality of tissue samples. The center platform includes a plurality of cryogenic discs, each having a plurality of bores for receiving object holders thereon, each object holder for receiving a frozen specimen thereon. The center platform cryogenic discs each have a channel system with intersecting peripheral, chordal and radial channels. A moistening tray includes structure having a closed, object holder wetting position and an open, object holder elevating position. Further disclosed is the use of a tissue orientation map, usable on temporary tattoos and tissue receiving sheets for specimen orientation and mapping of tumor roots.