Abstract: A field flow fractionator comprises a top plate, a bottom plate, a channel between the top plate and the bottom plate, an o-ring between the top plate and the bottom plate to form a first seal for the channel, and a membrane along a bottom of the channel. The top plate further comprises a lip extending vertically from the top plate to form a second seal for the channel by directly abutting the membrane.

Abstract: Described is a method, system, and computer program product of calculating electrophoretic mobility of a sample by extracting spectra.

Abstract: The present disclosure describes a method, system, and computer program product of calculating electrophoretic mobility of a sample by extracting frequency shift. In an embodiment, the method, system, and computer program product include receiving at least one frequency domain spectrum relating to light intensity detector data corresponding to a sample, truncating the spectrum to a region of interest, establishing a minimum fitter amplitude for what constitutes a peak, identifying possible distinct peaks in the region of interest, finding initial parameters for fitting at least one distinct peak among the possible distinct peaks, selecting a selected fitting model from among a plurality of fitting models with respect to the possible distinct peaks, and fitting the at least one distinct peak to the selected fitting model resulting in at least one fitted signal peak corresponding to the light intensity detector data, allowing for calculating electrophoretic mobility of particles in the sample.

Abstract: The present disclosure describes a circuit to control acousto-optic modulators for measuring electrophoretic mobility. In an embodiment, the circuit includes at least two channel amplifier circuits, a radio frequency generator logically coupled to the channel amplifier circuits, where the radio frequency generator is to output drive signals to the channel amplifier circuits, a synchronized data acquisition system logically coupled to the radio frequency generator, where the synchronized data acquisition system is to digitize an optical signal from a photo diode logically coupled to at least two acousto-optic modulators, and where one of the channel amplifier circuits is to drive one of acousto-optic modulators and the other of the channel amplifier circuits is to drive the other of the acousto-optic modulators, where the acousto-optic modulators and photo diode are to measure electrophoretic mobility of a sample.

Abstract: The present disclosure describes a laser driver circuit for measuring electrophoretic mobility of a sample. In an exemplary embodiment, the circuit includes a voltage regulator circuit to receive an input voltage and to output a stable output voltage, an operational amplifier electrically coupled to an output of the voltage regulator circuit, a transistor electrically coupled to an output of the operational amplifier and to the output of the voltage regulator circuit, a plurality of resistors electrically coupled to the operational amplifier and to the transistor, a plurality of capacitors electrically coupled to the operational amplifier, to the transistor, and to the plurality of resistors, and where the operational amplifier is to output a voltage to adjust a gate voltage of the transistor such that a drain source resistance of the transistor allows a controlled current to flow to a laser for measuring electrophoretic mobility of a sample.

Abstract: Described are examples of an apparatus for measuring electrophoretic mobility. The apparatus is configured as an optical interferometer wherein a sample to be analyzed in placed on one arm of the interferometer. Polarization-maintaining optical fibers and optical components maintain polarization stability and performance of the interferometer. Rayleigh scattered light from the sample is combined with light from the reference arm of the interferometer. The scattered light is shifted in frequency with a corresponding signal having a magnitude that is proportional to the velocity of the particles in solution. Particle velocity is a function of solvent viscosity, particle size, applied field strength, and electric charge. Measurement of electrophoretic mobility and particle size can be used to calculate the electric charge of the particles.

Abstract: The present disclosure describes an apparatus of managing solvent associated with a field flow fractionator. In an exemplary embodiment, the apparatus includes (1) a union assembly coupled to a detector flow output from at least one detector coupled to a field flow fractionator, and (2) a recycle and waste assembly coupled to an output of the union assembly and a channel cross flow output of the field flow fractionator.

Abstract: A continuous field flow fractionator includes a membrane, a bottom plate assembly and a top plate assembly that includes an inject port, an inlet port, at least one orthogonal flow inlet port, at least one orthogonal flow outlet port, and at least two sample outlet ports. The top plate assembly, the membrane, and the bottom plate assembly define a separation channel. The solvent flowing from the at least one orthogonal flow inlet port impinges on particles of a sample flowing between the inject port to the at least two sample outlet ports in the orthogonal flow region. The particles of the sample flow to the at least two sample outlet ports according to sizes of the particles of the sample, resulting in fractions of the sample in accordance with the sizes of the particles.

Abstract: A new liquid flow cell assembly for light scattering measurements is disclosed which utilized a floating manifold system. The assembly operates with minimal stacked tolerances by aligning the cell to the windows within a manifold and independently aligning the cell to the read head directly. This configuration enables the ability to replace the flow cell or the flow cell/manifold assembly within a light scattering instrument without the need to realign the flow through elements with the light scattering illumination source while still maintaining reproducible, quality data. Some embodiments employ wide bore cells which enable the measurement of process analytic technology (PAT) including online monitoring of reactions.

Abstract: The present disclosure describes an apparatus of managing solvent associated with a field flow fractionator. In an exemplary embodiment, the apparatus includes (1) a union assembly coupled to a detector flow output from at least one detector coupled to a field flow fractionator, and (2) a recycle and waste assembly coupled to an output of the union assembly and a channel cross flow output of the field flow fractionator.

Abstract: A new liquid flow cell assembly for light scattering measurements is disclosed which utilized a floating manifold system. The assembly operates with minimal stacked tolerances by aligning the cell to the windows within a manifold and independently aligning the cell to the read head directly. This configuration enables the ability to replace the flow cell or the flow cell/manifold assembly within a light scattering instrument without the need to realign the flow through elements with the light scattering illumination source while still maintaining reproducible, quality data. Some embodiments employ wide bore cells which enable the measurement of process analytic technology (PAT) including online monitoring of reactions.

Abstract: A new liquid flow cell assembly for light scattering measurements is disclosed which utilized a floating manifold system. The assembly operates with minimal stacked tolerances by aligning the cell to the windows within a manifold and independently aligning the cell to the read head directly. This configuration enables the ability to replace the flow cell or the flow cell/manifold assembly within a light scattering instrument without the need to realign the flow through elements with the light scattering illumination source while still maintaining reproducible, quality data. Some embodiments employ wide bore cells which enable the measurement of process analytic technology (PAT) including online monitoring of reactions.

Abstract: A new liquid flow cell assembly for light scattering measurements is disclosed which utilized a floating manifold system. The assembly operates with minimal stacked tolerances by aligning the cell to the windows within a manifold and independently aligning the cell to the read head directly. This configuration enables the ability to replace the flow cell or the flow cell/manifold assembly within a light scattering instrument without the need to realign the flow through elements with the light scattering illumination source while still maintaining reproducible, quality data. Some embodiments employ wide bore cells which enable the measurement of process analytic technology (PAT) including online monitoring of reactions.

Abstract: A new liquid flow cell assembly for light scattering measurements is disclosed which utilized a floating manifold system. The assembly operates with minimal stacked tolerances by aligning the cell to the windows within a manifold and independently aligning the cell to the read head directly. This configuration enables the ability to replace the flow cell or the flow cell/manifold assembly within a light scattering instrument without the need to realign the flow through elements with the light scattering illumination source while still maintaining reproducible, quality data. Some embodiments employ wide bore cells which enable the measurement of process analytic technology (PAT) including online monitoring of reactions.

Abstract: A new liquid flow cell assembly for light scattering measurements is disclosed which utilized a floating manifold system. The assembly operates with minimal stacked tolerances by aligning the cell to the windows within a manifold and independently aligning the cell to the read head directly. This configuration enables the ability to replace the flow cell or the flow cell/manifold assembly within a light scattering instrument without the need to realign the flow through elements with the light scattering illumination source while still maintaining reproducible, quality data. Some embodiments employ wide bore cells which enable the measurement of process analytic technology (PAT) including online monitoring of reactions.