Abstract: The present invention generally relates to libraries, compositions, methods, applications, kits and screens used in functional genomics that focus on gene function in a cell and that may use vector systems and other aspects related to Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas systems and components thereof. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system.

Abstract: A projectile launcher assembly includes a housing mounted to the deck of a maritime vessel. The housing has a plurality of launching wells each extending into the housing. A plurality of projectiles is positioned within a respective one of the launching wells. A launching unit is positioned in the housing to launch each of the projectiles outwardly from the respective launching wells thereby facilitating the plurality of projectiles to be dispersed into a body of water in which the maritime vessel is traveling. A plurality of buoyancy vests is each positioned within a respective one of the projectiles. Each of the buoyancy vests is inflatable when the projectiles are launched from the housing to facilitate the individual that has fallen overboard to wear one of the buoyancy vests to inhibit the individual from drowning.