Abstract: Embodiments of the present invention are directed to detecting misuse of contextual data, such as user data. In accordance with some embodiments of the present invention, artificial contextual data is created for artificial users and is, thereafter, provided to a bidder(s). Based on reception of artificial contextual data, bidders can place bids for presentation of advertisements. Bids placed by bidders receiving such artificial context data can be collected and analyzed. When bids appear to be atypically increased or decreased based on the artificial contextual data, a determination can be made that the bidder retains and/or misuses contextual data, such as user data.

Abstract: The present disclosure relates to methods using CRISPR-Cas13 enzyme, complexed with SARS-CoV-2 crRNA guide RNAs to detect and quantify the presence of SARS-CoV-2 RNA in a sample with enhanced specificity and sensitivity. These methods can be used to diagnose SARS-CoV-2 infection, quantify the concentration of SARS-CoV-2 RNA present in a sample, identify the presence of different SARS-CoV-2 splice variants, subtypes, or mutations, and to monitor reactivation of SARS-CoV-2 transcription.

Abstract: Disclosed example robotic welding systems include: a robotic manipulator configured to manipulate a welding torch; and a robot control system, comprising: a processor; and a machine readable storage medium comprising machine readable instructions which, when executed by the processor, cause the processor to, during a robotic welding procedure involving the robotic manipulator: prior to initiating an arc as part of the robotic welding procedure, identify an arc warning event; in response to the arc warning event, output at least one of a visual notification or an audible notification proximate to the robotic manipulator; and control the robotic manipulator to perform the robotic welding procedure involving initiating the arc using the welding torch.

Abstract: A mechanical steering wheel turn-end-stop device for use in steer-by-wire steering systems, comprising a connection element, which is configured for establishing a physical connection with a steering wheel shaft of a steering wheel, and which is configured for performing a coupled movement together with a physically connected steering wheel when the steering wheel is turned, and comprising a mechanical stop element, forming a limit stop for the connection element, preventing a further movement of the connection element in at least one movement direction and, via the established physical connection with the steering wheel shaft, preventing a further turning of the connected steering wheel in at least one turning direction is proposed.

Abstract: Disclosed example robotic welding systems include: a robotic manipulator configured to manipulate a welding torch; a first input device attached to the robotic manipulator and configured to receive rotational and translational inputs; a second input device attached to the robotic manipulator and configured to receive a masking input; and a robot control system, comprising: a processor; and a machine readable storage medium comprising machine readable instructions which, when executed by the processor, cause the processor to: in response to activation of the masking input via the second input device, masking at least a portion of the inputs received via the first input device; and in response to inputs to the first input device, move the robotic manipulator according to the inputs and based on whether the inputs are masked.

Abstract: Disclosed example robotic welding systems include: a robotic manipulator configured to manipulate a welding torch; and a robot control system, comprising: a processor; and a machine readable storage medium comprising machine readable instructions which, when executed by the processor, cause the processor to, during a robotic welding procedure involving the robotic manipulator: prior to initiating an arc as part of the robotic welding procedure, identify an arc warning event; in response to the arc warning event, output at least one of a visual notification or an audible notification proximate to the robotic manipulator; and control the robotic manipulator to perform the robotic welding procedure involving initiating the arc using the welding torch.

Abstract: Described herein are nucleic acid detection compositions and systems comprising an internal nuclease chain reaction (NCR) for signal amplification and methods of using these NCR-containing compositions and systems.

Abstract: Described herein are compositions and systems comprising activators of type III accessory nucleases and methods of using these compositions and systems.

Abstract: Described herein are nucleic acid detection compositions and systems comprising isothermal loop mediated signal amplification (LAMP) and methods of using these LAMP-based compositions and systems.

Abstract: A particulate filtration device is suggested, comprising an inlet for an inflow of a gas stream which is particle-laden, an outlet for an outflow of the gas stream having a significantly reduced particulate load, a main filter section, which is arranged within a flow path of the gas stream between the inlet and the outlet and which comprises a filtration medium, and a prefilter chamber, which is arranged upstream of the main filter section and which comprises an inertial filter element, having several perforations, wherein the perforations of the inertial filter element are arranged within the flow path of the gas stream in such a way that when the flow path of the gas stream is passing through the perforations, an inertial separation of the particulates from the mean flow path of the gas stream is achieved.

Abstract: A particulate filtration device is suggested, comprising an inlet for an inflow of a gas stream which is particle-laden, an outlet for an outflow of the gas stream having a significantly reduced particulate load, a main filter section, which is arranged within a flow path of the gas stream between the inlet and the outlet and which comprises a filtration medium, and a prefilter chamber, which is arranged upstream of the main filter section and which comprises an inertial filter element, having several perforations, wherein the perforations of the inertial filter element are arranged within the flow path of the gas stream in such a way that when the flow path of the gas stream is passing through the perforations, an inertial separation of the particulates from the mean flow path of the gas stream is achieved.

Abstract: The present disclosure relates to methods using CRISPR-Cas13 enzyme, complexed with SARS-CoV-2 crRNA guide RNAs to detect and quantify the presence of SARS-CoV-2 RNA in a sample with enhanced specificity and sensitivity. These methods can be used to diagnose SARS-CoV-2 infection, quantify the concentration of SARS-CoV-2 RNA present in a sample, identify the presence of different SARS-CoV-2 splice variants, subtypes, or mutations, and to monitor reactivation of SARS-CoV-2 transcription.

Abstract: The present disclosure provides variant Cas9 polypeptides, where a variant Cas9 polypeptide of the present disclosure comprises an internal insertion of a heterologous polypeptide. The present disclosure provides nucleic acids comprising nucleotide sequences encoding the variant Cas9 polypeptides. The present disclosure provides host cells comprising a variant Cas9 polypeptide of the present disclosure, or comprising a nucleic acid encoding a variant Cas9 polypeptide of the present disclosure. The present disclosure provides methods of binding and/or modifying a target nucleic acid, involving use of a variant Cas9 polypeptide of the present disclosure.

Abstract: Engineered microcompartment proteins, and related engineered microcompartment, vectors, cells compositions, methods and systems configured to provide within a cell one or more proteins non-native to the cell are described, wherein the one or more proteins non-native to the cell are contained in at least one engineered microcompartment within the cell.

Abstract: The present disclosure provides variant Cas9 polypeptides, where a variant Cas9 polypeptide of the present disclosure comprises an internal insertion of a heterologous polypeptide. The present disclosure provides nucleic acids comprising nucleotide sequences encoding the variant Cas9 polypeptides. The present disclosure provides host cells comprising a variant Cas9 polypeptide of the present disclosure, or comprising a nucleic acid encoding a variant Cas9 polypeptide of the present disclosure. The present disclosure provides methods of binding and/or modifying a target nucleic acid, involving use of a variant Cas9 polypeptide of the present disclosure.