Abstract: The instant disclosure provides reagent compounds, and antibody and oligonucleotide reagents, for use in a variety of assays, including immunoassays and nucleic acid hybridizations. The reagent compounds comprise a bridging antigen or bridging oligonucleotide and a latent crosslinker moiety, such as a tyramide moiety. The bridging antigens are recognizable by the antibody of a corresponding antibody reagent with high affinity, and the bridging oligonucleotides are complementary to the oligonucleotide of a corresponding oligonucleotide reagent. The antibody reagents and oligonucleotide reagents also comprise a crosslinker activation agent, such as a peroxidase enzyme. Reaction of the reagent compounds with the crosslinker activation agent results in the amplification of signal in assays for target cellular markers, including cellular antigens and nucleic acids.

Abstract: The present disclosure provides reagent compounds, reactive biopolymeric compounds, and methods of making and using these materials for the rapid and efficient synthesis and purification of biopolymeric compounds at low cost. The materials and methods yield highly pure synthetic biopolymeric compounds, including synthetic oligonucleotides and polypeptides, and reduce or eliminate the need for toxic solvents in the synthetic process.

Abstract: An incorrect transmission, of a record of data to a distributed ledger system, can be prevented. A first signal can be received. The first signal can include a first instruction to cause the record to be transmitted to the system. One or more items of information in the record can be determined. A delay of time to be elapsed, before a transmission of the record to the system, can be set in response to a receipt of the first signal and a determination of the one or more items. The transmission of the record to the system can be caused to occur after the delay has elapsed. The transmission of the record to the system can be prevented in response to a receipt of a second signal before the delay has elapsed. The second signal can include a second instruction that supersedes the first instruction.

Abstract: A method of screening a test compound, e.g., a fibrosis or IPF inhibitor, for induction of an unjammed-to-jammed transition (UJT) in fibrotic primary human bronchial epithelial cells (HBECs) isolated from a subject with a fibrotic lung disease includes culturing the fibrotic primary HBECs at an air-liquid interface for a time sufficient to provide a differentiated pseudostratified epithelium contacting the cultured cells with the test compound; and monitoring the motility of the cultured cells to identify the cultured cells as moving or stationary; wherein stationary cultured cells indicate that the test compound induces the UJT. Also included are methods of identifying lung fibrosis biomarkers.

Abstract: The instant disclosure provides reagent compounds, and antibody and oligonucleotide reagents, for use in a variety of assays, including immunoassays and nucleic acid hybridizations. The reagent compounds comprise a bridging antigen or bridging oligonucleotide and a latent crosslinker moiety, such as a tyramide moiety. The bridging antigens are recognizable by the antibody of a corresponding antibody reagent with high affinity, and the bridging oligonucleotides are complementary to the oligonucleotide of a corresponding oligonucleotide reagent. The antibody reagents and oligonucleotide reagents also comprise a crosslinker activation agent, such as a peroxidase enzyme. Reaction of the reagent compounds with the crosslinker activation agent results in the amplification of signal in assays for target cellular markers, including cellular antigens and nucleic acids.

Abstract: A website building system (WBS) includes an experiment management system to provide experiment management services to at least two tenancies where a single tenancy conducts at least one experiment independently without interfering with experiments conducted by other tenancies, the experiment management system includes an experiment creator to receive an experiment specification with source material from an external source and to create an experiment definition based on inspection and analysis of the source material; an experiment integrator to integrate the experiment definition as an experiment into the experiment management system; an experiment configurer to configure a variant and population selection criteria for the experiment; and an experiment manager to manage experiment conflicts between concurrently running experiments for the at least two tenancies at runtime according to the variant and population selection.

Abstract: An erroneous transmission, of a record of data, to a distributed ledger system can be prevented. A first operation can confirm that a format of a content within the record of data is correct. A second operation can confirm that formats of elements of a set of information, about authorizations used to cause the record of data to be communicated via the distributed ledger system, are correct. A third operation can confirm that subject matters of the elements and a subject matter of the record of data are the same. A fourth operation can retrieve credentials associated with entities that produced the elements. A fifth operation, using the credentials, can confirm that the entities that produced the elements were authorized to produce them. A sixth operation can confirm that the set of information is sufficient to authorize causing the record of data to be communicated via the distributed ledger system.

Abstract: Disclosed are biomarkers, methods and assay systems for the identification of poor prognosis of interstitial pneumonia (pulmonary fibrosis) in an individual diagnosed with suspected of having interstitial pneumonia.

Abstract: A system to detect and resolve conflicts between different experiments running on a website building system (WBS). The system includes at least one processor, a conflict resolver to check for interactions and conflicts between an experiment and at least one other existing experiment from a set of existing experiments in the WBS, when the experiment is set-up or integrated into the set of existing experiments. The conflict resolver either resolves the interactions and conflicts, or allows the interactions and conflicts to remain. The system also includes an experiment manager running on the at least one processor to manage and coordinate the experiment and the at least one other existing experiment when the experiment is running concurrently with the at least one other existing experiment, after set-up or integration of the experiment.

Abstract: Exemplary embodiments of the present disclosure can provide for a transparent panel that can have a featured image which can be surrounded by a pattern of repeating designs on a first side of the panel. A vinyl layer can be provided on the entire first side of the panel to assist in the featured image and repeating designs to be viewable on the second side of the panel (opposite of the first side). On the second opposite side of the panel, an area of the panel where the pattern of designs is visible is provided with a series of lens that can overly the repeating designs. In some exemplary embodiments, the lenses can project enlarged representations of the underlying repeating designs that appear to the eye as occupying a different plane than the featured image, creating a sense of depth within the image.

Abstract: Disclosed are biomarkers, methods and assay systems for the identification of poor prognosis of interstitial pneumonia (pulmonary fibrosis) in an individual diagnosed with suspected of having interstitial pneumonia.

Abstract: An incorrect copy of a record of data can be prevented from being transmitted to a distributed ledger system. A first file can be received and can include information, in audio or video form, with a description of a subject matter of the record of data and with an authorization to transmit the copy to the distributed ledger system. The first file can be sent to a device. A second file can be received from the device and can include information that confirms that the description of the subject matter, included in the first file, is correct, and that confirms that an entity, which controlled production of the first file, has permission to authorize causing the copy to be transmitted to the distributed ledger system. The correct copy can be caused, based on a receipt of the first and the second files, to be transmitted to the distributed ledger system.

Abstract: A method of screening a test compound, e.g., a fibrosis or IPF inhibitor, for induction of an unjammed-to-jammed transition (UJT) in fibrotic primary human bronchial epithelial cells (HBECs) isolated from a subject with a fibrotic lung disease includes culturing the fibrotic primary HBECs at an air-liquid interface for a time sufficient to provide a differentiated pseudostratified epithelium contacting the cultured cells with the test compound; and monitoring the motility of the cultured cells to identify the cultured cells as moving or stationary; wherein stationary cultured cells indicate that the test compound induces the UJT. Also included are methods of identifying lung fibrosis biomarkers.

Abstract: The present disclosure provides high-performance immunoreagents for use in a variety of immunologic assays and other related techniques. The immunoreagents comprise a primary antibody and a bridging antigen, wherein the bridging antigen is recognized by a detectable secondary antibody with high affinity. Also provided are compositions comprising panels of immunoreagents specific for multiple different target antigens and compositions comprising pairs of primary immunoreagents and their complementary detectable secondary antibodies. The paired primary immunoreagents and secondary antibodies are useful in a variety of immunologic assays, particularly in highly multiplexed assays, where the structure of the bridging antigen is varied in tandem with variation in the detectable secondary antibody, such that a multiplicity of immunoreagents are provided that are capable of simultaneously detecting a multiplicity of target antigens in a single assay.

Abstract: A geography-informed machine learning (GIML) model is trained on a first remote sensing dataset corresponding to a first geographic area and including a first set of atmospheric gas concentration data for at least one atmospheric gas, a first set of multispectral data, and a first set of spatially autocorrelated land use classifications. The GIML model receives input including a second remote sensing dataset corresponding to a second geographic area. The second remote sensing dataset includes a second set of atmospheric gas concentration data for the atmospheric gas, a second set of multispectral data, and a second set of spatially autocorrelated land use classifications. The GIML model generates, for the second geographic area, a plurality of predicted atmospheric gas concentration values for the atmospheric gas having a spatial resolution that is greater than a spatial resolution of the first and second sets of atmospheric gas concentration data.

Abstract: Generating one or more high-resolution atmospheric gas concentration maps using geography-informed machine learning includes obtaining a remote sensing dataset constrained by at least one temporal window and at least one spatial window defining a first geographic area. The remote sensing dataset includes at least a first set of atmospheric gas concentration data for a plurality of atmospheric gases. A training dataset is generated based on the remote sensing dataset. A machine learning model is trained with the training dataset to predict a plurality of atmospheric gas concentration values for at least one atmospheric gas of the plurality of atmospheric gases in a given geographic area and with a spatial resolution that is greater than a spatial resolution of atmospheric gas concentration data provided as an input to the machine learning module.

Abstract: This document describes techniques, apparatuses, and systems for a grid-based road model with multiple layers. An example road-perception system generates a roadway grid representation that includes multiple cells. The road-perception system uses data from multiple information sources to generate a road model that includes multiple layers. Each layer represents a roadway attribute of each cell in the grid and includes one or more layer hypotheses. In this way, the described techniques and systems can provide an accurate and reliable road model and quantify uncertainty therein.

Abstract: A differentiable physics model of a building is used that defines thermodynamic relationships between zones of the building and a heating, ventilation, and air-conditioning (HVAC) system. A physics-constrained, data driven model learns behaviors of controlled components of the HVAC system. For each of a series of times during online operation of the HVAC system, past state values are recorded representing a performance of the HVAC system in the building and past inputs to the HVAC system to maintain the states. The past state values and the past inputs are input into the differentiable physics model and the data driven model to: jointly update first parameters of the differentiable physics model and second parameters of the data driven model, e.g., using moving horizon estimation; and determine a current input to the controlled components, e.g., using model predictive control.

Abstract: The present disclosure provides high-performance immunoreagents for use in a variety of immunologic assays and other related techniques. The immunoreagents comprise a primary antibody and a bridging antigen, wherein the bridging antigen is recognized by a detectable secondary antibody with high affinity. Also provided are compositions comprising panels of immunoreagents specific for multiple different target antigens and compositions comprising pairs of primary immunoreagents and their complementary detectable secondary antibodies. The paired primary immunoreagents and secondary antibodies are useful in a variety of immunologic assays, particularly in highly multiplexed assays, where the structure of the bridging antigen is varied in tandem with variation in the detectable secondary antibody, such that a multiplicity of immunoreagents are provided that are capable of simultaneously detecting a multiplicity of target antigens in a single assay.